CNTF antibody

Principal name

CNTF antibody

Alternative names for CNTF antibody

Ciliary Neurotrophic Factor

SwissProt ID

O02732 (Pig), P14188 (Rabit), P20294 (Rat), P26441 (Human), P51642 (Mouse), Q02011 (Chick)

Gene ID

25707 (Cntf), 1270 (CNTF)

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Can (Canine)

Available hosts

Goat, Rabbit, Mouse, Chicken

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Functional assay (FN), Immunoprecipitation (IP), Neutralisation (NEUT), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of CNTF antibody

CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture. CNTF is synthesized by glia both in the CNS and PNS and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain. CNTF effects are mediated by a tripartite receptor complex consisting of two sigl-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR). CNTF can support the survival of many different cell populations within the PNS and the CNS. in vitro, CNTF promotes proliferation and neurol specifications in hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons, CNTF induces sprouting of cholinergic motor neurons and delays neural degeneration in genetic models of motor neuron disease. In addition, it is involved in the development stage of astrocytes and oligodendocytes.

General readings

Linker R., et al. (2009) Crit Rev Immunol. 29(1):43-68.
Stahl N., et al. (1994) J Neurobiol. 25(11):1454-66.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CNTF antibody

Western blot analysis of fetal brain lysates with anti-CNTF antibody (1:1000). This antibody identified a band of ~24kDa. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CNTF antibody

(0.5ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CNTF antibody

Western blot analysis of CNTF using recombinant human CNTF. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CNTF antibody

CNTF Antibody (Center) (Cat. #TA325126) western blot analysis in U87-MG cell line lysates (35ug/lane).This demonstrates the CNTF antibody detected the CNTF protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CNTF antibody

To detect hCNTF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCNTF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CNTF antibody

To detect hCNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CNTF (TA328279) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCNTF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CNTF antibody

To detect Rat CNTF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CNTF antibody

To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat CNTF (TA328575) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CNTF antibody

Western blot analysis using Anti-CNTF on: 1. human CNTF, 20ng. 2. hCardiotrophin-1, 20ng. 3. hLIF, 20ng. 4. mIL-6, 20 ng. 5. human CNTF, 20ng. Lanes 1-4. Anti-CNTF antibody, (1:200). Lane 5. Anti-CNTF, preincubated with the control peptide antigen. The specificity of the antibody is demonstrated in that it specifically recognizes CNTF while it does not reacts with the related cytokines Cardiotrophin-1, LIF and IL-6. Rabbit Purified Hu, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CNTF antibody

CNTF Mouse IgG1 AS23 Purified Hu E, IP, WB
0.5 mg / €400.00
  Acris Antibodies GmbH

CNTF antibody

CNTF Rabbit IgG Biotin Hu E
0.2 mg / €360.00
  Acris Antibodies GmbH

CNTF antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Biotin Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

CNTF antibody

CNTF Rabbit Biotin Rt E, WB
25 µg / €230.00
  Acris Antibodies GmbH

CNTF antibody

CNTF Rabbit Biotin Rt E, WB
50 µg / €340.00
  Acris Antibodies GmbH

CNTF antibody

CNTF Rabbit Aff - Purified Rt C, E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

CNTF antibody

CNTF Rabbit Aff - Purified Rt C, E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CNTF antibody

CNTF Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

CNTF antibody

CNTF Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

CNTF antibody

CNTF Rabbit Aff - Purified Hu C, E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

CNTF antibody

CNTF Rabbit Aff - Purified Hu C, E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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