This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Provost. P., et al. (2001) Biochem J 359(1): 255-263 Dai. H., et al. (2006) Biochim Biophys Acta 1764(11): 1688-1700 Esser. J., et al. (2010) Biochem J 425(1): 265-274
Immunohistochemical of paraffin-embedded human lung cancer using 10781-1-AP(COTL1 antibody) at dilution of 1:50 (under 40x lens)
Human Thymus; WB Suggested Anti-COTL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Thymus; COTL1 antibody - N-terminal region (ARP55374_P050) in Human Thymus cells using Western Blot
Western blot analysis of COTL1 (arrow) using rabbit polyclonal COTL1 Antibody (C-term) (GTX81005). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COTL1 gene.
Formalin-fixed and paraffin-embedded human spleen tissue reacted with COTL1 Antibody (C-term)(GTX81005), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
COTL1 Antibody (C-term) (GTX81005) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
The cell lysates of SW480(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COTL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.