Coagulation factor VIII (F8) antibody

Principal name

Coagulation factor VIII (F8) antibody

Alternative names for Coagulation factor VIII (F8) antibody

Procoagulant component, Antihemophilic factor, F8C, AHF

SwissProt ID

O18806 (Canfa), P00451 (Human), P12263 (Pig), Q06194 (Mouse)

Gene ID

2157 (F8), 14069

Available reactivities

Hu (Human), Por (Porcine), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Eq (Equine), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Rabbit, Chicken, Sheep, Rat

Available applications

Enzyme Immunoassay (E), Functional assay (FN), Western blot / Immunoblot (WB), Frozen Sections (C), Radioimmunoassay (R), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Coagulation factor VIII (F8) antibody

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. It is an extracellular factor. Defects in F8 are the cause of hemophilia A (HEMA). HEMA is a common recessive X linked coagulation disorder. The frequency of hemophilia A is 1-2 in 10,000 male births in all ethnic groups. About 50% of patients have severe hemophilia A with F8C activity less than 1% of normal; they have frequent spontaneous bleeding into joints, muscles and internal organs. Moderately severe hemophilia A occurs in about 10% of patients; F8C activity is 2-5% of normal, and there is bleeding after minor trauma. Mild hemophilia A, which occurs in 30-40% of patients, is associated with F8C activity of 5-30% and bleeding occurs only after significant trauma or surgery. Of particular interest for the understanding of the function of F8C is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8C in their plasma (at least 30% of normal), but the protein is nonfunctional; i.e., the F8C activity is much less than the plasma protein level. CRM reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.

General readings

General / background references:Takase T et al. Production of factor VIII deficient plasma by immunodepletion using three monoclonal antibodies. Br J Haematol 66:497-502 (1987). PubMed PMID: 3117089
Rotblat F et al. Purification of human factor VIII:C and its characterization by Western blotting using monoclonal antibodies. Biochemistry 24:4294-300 (1985). PubMed PMID: 2413885

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Coagulation factor VIII (F8) antibody

Anti-F8 / Factor VIII antibody IHC of human skeletal muscle and capillaries. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Sheep IgG Purified Hu E, EMSA
0.5 mg / €345.00
  OriGene Technologies, Inc.

Coagulation factor VIII (F8) antibody

Western blot analysis on HuvEc cell lysate using Factor VIII Antibody Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Coagulation factor VIII (F8) antibody

WB Suggested Anti-F8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 721_B cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Rb, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

Coagulation factor VIII (F8) antibody

ELISA: Factor VIII Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Coagulation factor VIII (F8) antibody

Coagulation factor VIII (F8) Mouse IgG1 101 Purified Hu E, WB
0.2 mg / €440.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) (A2 domain) antibody

Coagulation factor VIII (F8) Mouse IgG1 012 Purified Hu E, WB
0.5 mg / €710.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) antibody

Coagulation factor VIII (F8) Mouse IgG1 RFFVIIIC/5 Purified Hu E, WB
0.5 mg / €630.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) antibody

Western blot analysis using F8 antibody Cat.-No AM06283SU-N against truncated Trx-F8 recombinant protein (Lane 1). Mouse IgG1 5E9B2 Ascites Hu WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) antibody

Human Uterus, Vessels (formalin-fixed, paraffin-embedded) stained with F8 antibody AM20504PU-N at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 F8 2.2.9 Purified Hu P
50 µg / €470.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) antibody

Western blot (WB) analysis of F8 antibody (Cat.-No.: AP06114PU-N) at 1/500 dilution Lane1:HepG2 whole cell lysate Lane2:Mouse liver tissue lysate Lane3:Rat liver tissue lysate Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) antibody

Coagulation factor VIII (F8) Mouse IgG2a 2A5 Purified Hu E
0.2 mg / €360.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) antibody

Coagulation factor VIII (F8) Mouse IgG2a 104 Ascites Hu E, FN, WB
1 ml / €570.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) antibody

Coagulation factor VIII (F8) Mouse IgG2a MH104 Purified Hu C, E, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) antibody

Recombinant Human Factor VIII detected with Mouse anti Human Factor VIII antibody SM2285P Mouse IgG1 RFFVIIIC/8 Purified Hu, Por E, R, WB
0.5 mg / €550.00
  Acris Antibodies GmbH

Coagulation factor VIII (F8) antibody

Coagulation factor VIII (F8) Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

Coagulation factor VIII (F8) antibody

Immunoprecipitation of F8 transfected lysate using anti-F8 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with F8 MaxPab mouse polyclonal antibody (B01) (H00002157-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Coagulation factor VIII (F8) antibody

Western Blot analysis of F8 expression in transfected 293T cell line (H00002157-T01) by F8 MaxPab polyclonal antibody.

Lane 1: F8 transfected lysate(24.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Coagulation factor VIII (F8) antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and F8. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-F8 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2b 1E8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Coagulation factor VIII (F8) antibody

Coagulation factor VIII (F8) Rat IgG Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Coagulation factor VIII (F8) antibody

Coagulation factor VIII (F8) Mouse IgG1 Hu E
0.1 mg / €390.00
  Abnova Taiwan Corp.

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