Coatomer subunit alpha (COPA) antibody

Principal name

Coatomer subunit alpha (COPA) antibody

Alternative names for Coatomer subunit alpha (COPA) antibody

Alpha-coat protein, HEP-COP

SwissProt ID

P53621 (Human), Q27954 (Bovin), Q8CIE6 (Mouse)

Gene ID

1314 (COPA)

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Chk (Chicken), Ms (Mouse), Ze (Zebrafish), African clawed frog, Por (Porcine), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit), Ye (Yeast)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of Coatomer subunit alpha (COPA) antibody

The coatomer protein complex, alpha subunit (COPA) is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. This interaction mediates biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors, small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

General readings

General / background references:Futatsumori M et al. Identification and characterization of novel isoforms of COP I subunits. J Biochem (Tokyo) 128:793-801 (2000). PubMed PMID: 11056392
Shimoni Y et al. Lst1p and Sec24p cooperate in sorting of the plasma membrane ATPase into COPII vesicles in Saccharomyces cerevisiae. J Cell Biol 151:973-84 (2000). PubMed PMID: 11086000
Barlowe C Traffic COPs of the early secretory pathway. Traffic 1:371-7 (2000). PubMed PMID: 11208122

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Coatomer subunit alpha (COPA) antibody

(0.5ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Coatomer subunit alpha (COPA) antibody

WB Suggested Anti-COPA Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysate COPA is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Coatomer subunit alpha (COPA) antibody

WB Suggested Anti-COPA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: 721_B cell lysateCOPA is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Coatomer subunit alpha (COPA) antibody

Coatomer subunit alpha (COPA) Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Coatomer subunit alpha (COPA) antibody

Human HeLa; WB Suggested Anti-COPA Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate. COPA is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; COPA antibody - N-terminal region (ARP51893_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Coatomer subunit alpha (COPA) antibody

Human 721_B; WB Suggested Anti-COPA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 721_B cell lysate. COPA is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; COPA antibody - middle region (ARP51894_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Coatomer subunit alpha (COPA) antibody

Figure 1 illustrates immunolocalization of COP alpha in HeLa cells using NB120-2913. Rabbit IgG Aff - Purified Hu, Rt ICC/IF, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Coatomer subunit alpha (COPA) antibody

Western Blot: alpha COP I Antibody [NBP1-59297] - This Anti-COPA antibody was used in Western Blot of Hela tissue lysate at a concentration of 0.5ug/ml. Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

Coatomer subunit alpha (COPA) antibody

Western Blot: alpha COP I Antibody [NBP1-59298] - This Anti-COPA antibody was used in Western Blot of 721_B tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

Coatomer subunit alpha (COPA) antibody

Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity with a granular pattern in exocrine cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Coatomer subunit alpha (COPA)

Coatomer subunit alpha (COPA) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Coatomer subunit alpha (COPA)

Coatomer subunit alpha (COPA) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

COPA overexpression lysate

COPA overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

13 Item(s)

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