Coatomer subunit beta (COPB) antibody

Principal name

Coatomer subunit beta (COPB) antibody

Alternative names for Coatomer subunit beta (COPB) antibody

COPB1, MSTP026, Beta-COP, Beta-coat protein, Coatomer protein complex subunit beta

SwissProt ID

A0JN39 (Bovin), P23514 (Rat), P53618 (Human), Q5ZIA5 (Chick), Q9JIF7 (Mouse)

Gene ID

1315 (COPB1), 114023 (Copb1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Av (Birds), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey), Hst (Hamster), Dros (Drosophila), Por (Porcine), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of Coatomer subunit beta (COPB) antibody

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP I) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

General readings

General / background references:Kreis TE et al. COPs regulating membrane traffic. Annu Rev Cell Dev Biol 11:677-706 (1995). PubMed PMID: 8689572
Griffiths G et al. Immunocytochemical localization of beta-COP to the ER-Golgi boundary and the TGN. J Cell Sci 108 (Pt 8):2839-56 (1995). PubMed PMID: 7593324
Whitney JA et al. Cytoplasmic coat proteins involved in endosome function. Cell 83:703-13 (1995). PubMed PMID: 8521487
Pepperkok R et al. Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo. Cell 74:71-82 (1993). PubMed PMID: 8334707
Oprins A et al. Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas. J Cell Biol 121:49-59 (1993). PubMed PMID: 8458872
Narula N et al. Identification of a 200-kD, brefeldin-sensitive protein on Golgi membranes. J Cell Biol 117:27-38 (1992). PubMed PMID: 1556155
Duden R et al. Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin. Cell 64:649-65 (1991). PubMed PMID: 1840503
Ktistakis NT et al. PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A. J Cell Biol 113:1009-23 (1991). PubMed PMID: 1710224
Donaldson JG et al. Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action. J Cell Biol 111:2295-306 (1990). PubMed PMID: 2277061
Allan VJ & Kreis TE A microtubule-binding protein associated with membranes of the Golgi apparatus. J Cell Biol 103:2229-39 (1986). PubMed PMID: 3536963

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Coatomer subunit beta (COPB) antibody

WB Suggested Anti-COPB1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Coatomer subunit beta (COPB) antibody

Detection limit for recombinant GST tagged COPB is approximately 0.3ng/ml as a capture antibody. Mouse IgG2b 3E10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Coatomer subunit beta (COPB) antibody

Human Liver; WB Suggested Anti-COPB1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Liver; COPB1 antibody - middle region (ARP52371_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Coatomer subunit beta (COPB) antibody

Western Blot: Beta COP Antibody [NB120-2915] - Figure 1 illustrates Western blot detection of Beta-COP from PC-12 cell lysates using NB120-2915. Rabbit IgG Aff - Purified Rt WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Coatomer subunit beta (COPB) antibody

Coatomer subunit beta (COPB) Mouse IgG1 maD Ascites Hu, Mky, Rt ICC/IF, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Coatomer subunit beta (COPB) antibody

Coatomer subunit beta (COPB) Mouse IgG1 M3A5 Ascites Av, Bov, Can, Chk, Hu, Mky, Rt ICC/IF, IP, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

Coatomer subunit beta (COPB) antibody

Western Blot: beta COP Antibody [NBP1-53091] - This Anti-COPB1 antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Bov, Can, Chk, Dros, Hu, Ms, Por, Rt, Xen, Ze WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Coatomer subunit beta (COPB) (transcript variant 1)

Coatomer subunit beta (COPB) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Coatomer subunit beta (COPB) (transcript variant 2)

Coatomer subunit beta (COPB) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Coatomer subunit beta (COPB)

Coatomer subunit beta (COPB) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Coatomer subunit beta (COPB)

Coatomer subunit beta (COPB) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

COPB1 overexpression lysate

COPB1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

COPB1 overexpression lysate

COPB1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

COPB1 overexpression lysate

COPB1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

14 Item(s)

per page
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