Collagen type III alpha 1 chain antibody

Principal name

Collagen type III alpha 1 chain antibody

Alternative names for Collagen type III alpha 1 chain antibody

COL3A1

SwissProt ID

P02461 (Human), P04258 (Bovin), P08121 (Mouse), P12105 (Chick), P13941 (Rat)

Gene ID

396340 (COL3A1), 1281, 12825 (Col3a1), 84032

Available reactivities

Chk (Chicken), Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Rb (Rabbit), Mam (Mammalia), Can (Canine), Por (Porcine), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), Paraffin Sections (P), Western blot / Immunoblot (WB), Dot blot (Dot), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of Collagen type III alpha 1 chain antibody

Antibodies to human collagen type III are raised in goats which are numerously immunized with extensively purified native collagen type III extracted from human skin into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-specific antibodies. Next, the antisera fraction is absorbed with immunobilized collagen types I, II, IV and V to remove cross-reactive antibodies to antigenic determinants common for various collagen types. The affinity purified antibody PS062 is obtained by binding to immobilized native human collagen type III (the antigen used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization. Specificity was ascertained by competition ELISA. Complete inhibition is found if the antibody was preincubated with collagen type III. Inhibition by other types of collagens was observed only at 20-50 times higher concentration. No inhibition was found with fibronectin, fibrinogen and laminin. Characteristic immunostaining pictures of frozen sections of human kidney, liver, skin and heart are produced to certify batch quality.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Collagen type III alpha 1 chain antibody

Immunohistochemical staining of human skin paraffin sections using Collagen type III antibody clone III-53. Mouse IgG1 III-53 Purified Hu, Rb C, P, WB
0.1 mg / €190.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Immunohistochemistry of tissue of right lobe of the liver section (Formalin-fixed, Paraffin-embedded). A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow) using Collagen type III alpha 1 chain antibody Cat.-No. R1040 at 1:500 for 4°C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody and DAB as chromogen. Nuclei were counterstained purple with hematoxylin. Rabbit IgG Aff - Purified Bov, Hu C, E, IP, P, WB
0.1 mg / €290.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Immunohistochemistry analysis of Collagen III antibody (Cat.-No AP06517PU-N) in paraffin-embedded human pancreas tissue. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Staining COL3A1 in Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Bov, Hu E, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov E, ICC/IF, P
0.5 ml / €580.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. TA308777 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type III alpha 1 chain antibody

Western blot analysis of extracts from A549 cells, treated with etoposide (25uM, 24hours), using Collagen III a1 (Cleaved-Gly1221) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type III alpha 1 chain antibody

Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using Collagen III antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type III alpha 1 chain antibody

Western blot analysis of Collagen III expression in HT1080 Whole cell lysate Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Collagen type III alpha 1 chain antibody

Lane 1: HT1080 whole cell lysate
Lane 2: colo320 whole cell lysate Mouse IgG1 Col-29 Purified Hu, Ms, Rt C, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Collagen type III alpha 1 chain Mouse IgM MC3-HA Purified Hu C, E, R, WB
1 ml / €370.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Collagen type III alpha 1 chain Goat Purified Hu C, E, WB
0.2 mg / €430.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Collagen type III alpha 1 chain Rabbit IgG Purified Rt C, E, ICC/IF
50 µg / €430.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Formalin-Fixed, Paraffin-Embedded tissue stained with Anti-Collagen III antibody Cat.-No AP09013BT-N after heat-induced antigen retrieval. Rabbit Biotin Bov, Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Collagen type III alpha 1 chain Rabbit Aff - Purified Rt C, Dot, E, ICC/IF
1 ml / €340.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Collagen type III alpha 1 chain Rabbit Aff - Purified Rt C, Dot, E, ICC/IF
1 ml / €340.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Collagen type III alpha 1 chain Mouse IgM NLI/42 Ig Fraction Hu C, E
1 ml / €350.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Collagen type III alpha 1 chain Mouse IgG1 8D1-8C7 Purified Hu C, E, ICC/IF, P, R
0.1 ml / €410.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Collagen type III alpha 1 chain Rabbit Azide Free Chk C, E, ICC/IF
0.5 ml / €930.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain antibody

Collagen type III alpha 1 chain Rabbit Purified Bov C, E, P, R
0.5 ml / €600.00
  Acris Antibodies GmbH

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