Collagen type IV antibody

Principal name

Collagen type IV antibody

Alternative names for Collagen type IV antibody

COL4A1

SwissProt ID

P02462 (Human), P02463 (Mouse), Q7SIB2 (Bovin)

Gene ID

12826 (Col4a1), 1282 (COL4A1)

Available reactivities

Ms (Mouse), Bov (Bovine), Por (Porcine), Rt (Rat), Hu (Human), Can (Canine), Fe (Feline), Fi (Fish), Mam (Mammalia), Orang-Utan, Prim (Primate), Chk (Chicken)

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Dot blot (Dot), Flow Cytometry (F)

Background of Collagen type IV antibody

Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas. This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

  1. Quick-Filters:
  2. Cited (4)
  3. Overnight Delivery (2)

Items 1 to 20 of 65 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Collagen type IV antibody

Staining of FFPE Human skin (20x and 40x) with Collagen type IV Antibody Cat.-No AF5910 (Clone IV-4H12) at 2 µg/ml. Antibody positive in fibillar collagen and in epithelial cells of basement membrane. Mouse IgG1 IV-4H12 Purified Hu C, P, WB
0.1 mg / €190.00
  Acris Antibodies GmbH

Collagen type IV antibody

R1041 Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit Aff - Purified Bov, Hu, Mam C, E, IP, P, WB
0.1 mg / €290.00
  Acris Antibodies GmbH

Collagen type IV antibody

R1041B Biotin conjugated Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: Collagen IV antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen IV is extracellular. Staining: Collagen IV as precipitated red signal with hematoxylin purple nuclear counterstain. Rabbit Biotin Bov, Hu, Mam C, E, IP, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Collagen type IV antibody

R1041 Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit Aff - Purified Bov, Hu, Mam C, E, IP, P, WB
0.5 mg / €780.00
  Acris Antibodies GmbH

Collagen type IV antibody

Western blot analysis of extracts from HeLa cells, using Collagen IV antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type IV antibody

Predicted cell location: Cytoplasm;Basement membrane;Extracellular space;.Extracellular matrix. Positive control: Human lung and breast cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using COL4A1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type IV antibody

Predicted cell location: Cytoplasm;Basement membrane;. Extracellular space;Extracellular matrix. Positive control: Human ovarian and breast cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using COL4A1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type IV antibody

Western blot analysis on HeLa cell lysate using Collagen IV Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Collagen type IV antibody

Collagen type IV Mouse IgG1 2F11 Biotin Hu C, E
0.1 mg / €390.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgG1 2F11 FITC Hu C, E
0.1 mg / €430.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgG1 2F11 Purified Hu C, E
0.1 mg / €340.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgG1 2F11 PE Hu C, E
0.1 mg / €520.00
  Acris Antibodies GmbH

Collagen type IV antibody

Immunohistochemistry on Human mammary cancer Mouse IgG1 Col-30 Purified Hu C, P
0.1 mg / €340.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgM MC4-HA Purified Hu C, E
1 ml / €350.00
  Acris Antibodies GmbH

Collagen type IV antibody

Immunohistochemistry on paraffin section of human skin Mouse IgG1 1043 Purified Bov, Hu, Por C, F, ICC/IF, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Goat Purified Hu C, E, ICC/IF, WB
0.2 mg / €550.00
  Acris Antibodies GmbH

Collagen type IV antibody

Immunohistochemistry analyzes of Collagen IV antibody Cat-No AP06069PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Collagen type IV antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bov, Hu, Mam E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Collagen type IV antibody

Human Spleen (formalin-fixed, paraffin-embedded) stained with Collagen IV antibody AP22814AF-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Azide Free Hu C, E, ICC/IF, P
25 µg / €470.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgM NLI/53 Purified Hu C, E, P
1 ml / €350.00
  Acris Antibodies GmbH

Items 1 to 20 of 65 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn