Collagen type IV antibody

Principal name

Collagen type IV antibody

Alternative names for Collagen type IV antibody

COL4A1

SwissProt ID

P02462 (Human), P02463 (Mouse), Q7SIB2 (Bovin)

Gene ID

1282 (COL4A1), 12826 (Col4a1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Mam (Mammalia), Por (Porcine), Can (Canine), Fe (Feline), Fi (Fish), Rt (Rat), Orang-Utan, Chk (Chicken)

Available hosts

Mouse, Goat, Rabbit

Available applications

Paraffin Sections (P), Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), Dot blot (Dot), Flow Cytometry (F)

Selected product citations ‐ of R1041

1. Sasse P, Malan D, Fleischmann M, Roell W, Gustafsson E, Bostani T, et al. Perlecan is critical for heart stability. Cardiovasc Res. 2008 Dec 1;80(3):435-44. doi: 10.1093/cvr/cvn225. Epub 2008 Aug 10. PubMed PMID: 18694874.
2. Behrens DT, Villone D, Koch M, Brunner G, Sorokin L, Robenek H, et al. The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens. J Biol Chem. 2012 May 25;287(22):18700-9. doi: 10.1074/jbc.M111.336073. Epub 2012 Apr 9. PubMed PMID: 22493504.
3. Rodriguez-Teja M, Gronau JH, Breit C, Zhang YZ, Minamidate A, Caley MP, et al. AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival. J Pathol. 2015 Mar;235(4):581-92. doi: 10.1002/path.4485. Epub 2014 Dec 24. PubMed PMID: 25408555. 
4. Rodriguez-Teja, M;Breit, C;Clarke, M;Talar, K;Wang, K;Mohammad, MA;Pickwell, S;Etchandy, G;Stasiuk, GJ;Sturge, J;2016How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases. J Vis Exp  115. PubMed PMID: 27684203.

Background of Collagen type IV antibody

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Collagen type IV antibody

Staining of FFPE Human skin (20x and 40x) with Collagen type IV Antibody Cat.-No AF5910 (Clone IV-4H12) at 2 µg/ml. Antibody positive in fibillar collagen and in epithelial cells of basement membrane. Mouse IgG1 IV-4H12 Purified Hu C, P, WB
0.1 mg / €190.00
  Acris Antibodies GmbH

Collagen type IV antibody

R1041 Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit Aff - Purified Bov, Hu, Mam C, E, IP, P, WB
0.1 mg / €290.00
  Acris Antibodies GmbH

Collagen type IV antibody

R1041B Biotin conjugated Collagen IV antibody (Lot 25440, 1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: Collagen IV antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen IV is extracellular. Staining: Collagen IV as precipitated red signal with hematoxylin purple nuclear counterstain. Rabbit Biotin Bov, Hu, Mam C, E, IP, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Collagen type IV antibody

  Rabbit polyclonal Collagen IV antibody  
Western blot analysis of extracts from HeLa cells, using Collagen IV antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type IV antibody

  Anti-COL4A1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm;Basement membrane;Extracellular space;.Extracellular matrix. Positive control: Human lung and breast cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using COL4A1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type IV antibody

  Anti-COL4A1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm;Basement membrane;. Extracellular space;Extracellular matrix. Positive control: Human ovarian and breast cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using COL4A1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type IV antibody

  Rabbit Polyclonal Anti-Collagen IV Antibody  
Western blot analysis on HeLa cell lysate using Collagen IV Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Collagen type IV antibody

Collagen type IV Mouse IgG1 2F11 Biotin Hu C, E
0.1 mg / €390.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgG1 2F11 FITC Hu C, E
0.1 mg / €430.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgG1 2F11 Purified Hu C, E
0.1 mg / €340.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgG1 2F11 PE Hu C, E
0.1 mg / €520.00
  Acris Antibodies GmbH

Collagen type IV antibody

Immunohistochemistry on Human mammary cancer Mouse IgG1 Col-30 Purified Hu C, P
0.1 mg / €340.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgM MC4-HA Purified Hu C, E
1 ml / €350.00
  Acris Antibodies GmbH

Collagen type IV antibody

Immunohistochemistry on paraffin section of human skin Mouse IgG1 1043 Purified Bov, Hu, Por C, F, ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Goat Purified Hu C, E, ICC/IF, WB
0.2 mg / €550.00
  Acris Antibodies GmbH

Collagen type IV antibody

Immunohistochemistry analyzes of Collagen IV antibody Cat-No AP06069PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Collagen type IV antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bov, Hu E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgM NLI/53 Purified Hu C, E, P
1 ml / €350.00
  Acris Antibodies GmbH

Collagen type IV antibody

Swine Kidney, Paraffin Section stained with type IV Collagen Antibody (Clone CIV22) Cat.-No BM4067 Mouse IgG1 CIV22 Purified Bov, Hu, Por C, P
0.1 mg / €500.00
  Acris Antibodies GmbH

Collagen type IV antibody

Collagen type IV Mouse IgG2b 1042 Ascites Hu C, E, P, WB
1 ml / €360.00
  Acris Antibodies GmbH

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