Collagen type V antibody

Principal name

Collagen type V antibody

Alternative names for Collagen type V antibody

COL5A1

SwissProt ID

O88207 (Mouse), P20908 (Human), Q9JI03 (Rat)

Gene ID

85490 (Col5a1), 1289 (COL5A1)

Available reactivities

Rt (Rat), Chk (Chicken), Bov (Bovine), Ms (Mouse), Hu (Human), Rb (Rabbit), Mam (Mammalia), Can (Canine), Por (Porcine), Sh (Sheep), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Radioimmunoassay (R), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Dot blot (Dot), Immunoelectron Microscopy (EM)

Background of Collagen type V antibody

Antibodies to human collagen type V are raised in goats which are numerously immunized with extensively purified native collagen type V extracted from human placenta into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-specific antibodies. Next, the antisera fraction is absorbed with immunobilized collagen types I, II, III, and IV to remove cross-reactive antibodies to antigenic determinants common for various collagen types. The affinity purified antibody PS064 is obtained by binding to immobilized native human collagen type V (the antigen used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization. Specificity was ascertained by competition ELISA. Complete inhibition was found if the antibo

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Collagen type V antibody

Staining of FFPE Human lung parenchyma (10x and 40x) with Collagen type V Antibody Cat.-No AF6110 (Clone V-3C9) at 2 µg/ml. Antibody positive in epithelial cells of basement membrane  and in alveolar interstitium. Mouse IgM V-3C9 Purified Hu, Rb, Rt C, E, P, WB
0.1 mg / €190.00
  Acris Antibodies GmbH

Collagen type V antibody

Collagen type V Mouse IgM MC5-HA Purified Hu C, E, R
1 ml / €370.00
  Acris Antibodies GmbH

Collagen type V (collagen v alpha-1 chain) (alpha-1) antibody

Collagen V alpha-1 chain N-propeptide -VR domain (COL5A1) antibody (6A7) Cat.-No AM10160PU-N EM staining of human dermal fibroblasts cells (5 days). Mouse IgG1 6A7 Purified Hu E, EM, ICC/IF, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

Collagen type V antibody

Western blot analysis of extracts from HeLa cells, using Collagen V a1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type V antibody

Predicted band size: 184 kDa . Positive control: Lung tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Collagen type V antibody

WB Suggested Anti-COL5A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Collagen type V antibody

Western blot analysis on HeLa cell lysate using Collagen Valpha 1 Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Collagen type V antibody

Collagen type V Mouse IgG1 18G5 Purified Hu E, ICC/IF, IP, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

Collagen type V antibody

Collagen V alpha-1 chain N-propeptide -NC2 domain antibody (13F6) (Cat.-No AM10159PU-N) Anti-COL5A1 antibody ICC staining of human derma fibroblasts (24h). Mouse IgG1 13F6 Purified Hu E, ICC/IF, IP, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

Collagen type V antibody

Collagen type V Goat Purified Hu C, E, ICC/IF, WB
0.2 mg / €590.00
  Acris Antibodies GmbH

Collagen type V alpha 1 chain antibody

Human Kidney (formalin-fixed, paraffin-embedded) stained with COL5A1 antibody AP09015BT-N at 1.25 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Biotin Bov, Hu E, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Collagen type V antibody

Immunohistochemistry (IHC) analyzes of COL5A1 antibody (Cat.-No.: AP20478PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Collagen type V (N-term) antibody

COL5A1 antibody (N-term) (Cat. #AP51017PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL5A1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Collagen type V antibody

Collagen type V Mouse IgM NLI/64 Ig Fraction Hu C, E
1 ml / €350.00
  Acris Antibodies GmbH

Collagen type V antibody

Collagen type V Rabbit Purified Rt E, ICC/IF
0.5 ml / €930.00
  Acris Antibodies GmbH

Collagen type V antibody

Collagen type V Rabbit Purified Chk C, E, ICC/IF, P, R
0.5 ml / €680.00
  Acris Antibodies GmbH

Collagen type V antibody

Collagen type V Rabbit Purified Bov, Ms, Rt C, E, ICC/IF, P, R
0.5 ml / €680.00
  Acris Antibodies GmbH

Collagen type V antibody

Collagen type V Rabbit Purified Hu C, E, ICC/IF, P, R
0.5 ml / €600.00
  Acris Antibodies GmbH

Collagen type V antibody

Anti Collagen type V Antibody (Cat.-No R1042 Lot 22063, 1:200, 45 min RT) showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.  Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit IgG Purified Bov, Hu, Mam C, E, IP, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Collagen type V antibody

Anti-Collagen type V Antibody (Cat.-No R1042 Lot 22063, 1/200, 45 min RT) showed strong staining in FFPE sections of Human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit IgG Biotin Bov, Hu, Mam C, E, IP, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

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