Collagen type VI antibody

Principal name

Collagen type VI antibody

Alternative names for Collagen type VI antibody

COL6A1

SwissProt ID

P12109 (Human), P20785 (Chick), Q04857 (Mouse)

Gene ID

1291 (COL6A1), 12833 (Col6a1)

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Rb (Rabbit), Mam (Mammalia), Can (Canine), Eq (Equine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Dot blot (Dot)

Background of Collagen type VI antibody

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. For immunization collagens from human and bovine placenta and cartilage extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

General readings

Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990 (Conjugation).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Collagen type VI antibody

Staining of FFPE Human testis parenchyma (20x and 40x) with Collagen Type VI Antibody Cat.-No AF6210 (Clone VI-26) at 2 µg/ml. Antibody positive in the cytoplasm of interstitial stromal fibroblasts. Mouse IgG1 VI-26 Purified Hu, Rb C, P, WB
0.1 mg / €190.00
  Acris Antibodies GmbH

Collagen type VI antibody

AP07844PU-N Collagen type VI antibody staining of Formalin-Fixed Paraffin-Embedded Human Lung tissue. Rabbit Aff - Purified Bov, Hu E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Collagen type VI antibody

  Rabbit Polyclonal Anti-COL6A1 Antibody  
WB Suggested Anti-COL6A1 Antibody Titration: 5% Milk; ELISA Titer: dilution: 1: 500; Positive Control: human LCL Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Collagen type VI antibody

Collagen type VI Goat Purified Hu C, E, ICC/IF, WB
0.2 mg / €600.00
  Acris Antibodies GmbH

Collagen type VI alpha 1 chain antibody

Western blot analysis of COL6A1 antibody (C-term) (AP17225PU-N) in Y79 cell line lysates (35 µg/lane). COL6A1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Collagen type VI alpha 1 chain antibody

Western blot analysis of COL6A1 antibody (N-term) (AP17226PU-N) in A2058 cell line lysates (35 µg/lane). COL6A1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Collagen type VI antibody

Human Lung (formalin-fixed, paraffin-embedded) stained with Collagen VI antibody AP22819PU-N at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu, Mam E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Collagen type VI antibody

Formalin-Fixed, Paraffin-Embedded Human Placenta tissue stained with AP22934AF-N at 10 µg/ml after heat-induced antigen retrieval. Goat Azide Free Hu C, Dot, E, ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

Collagen type VI antibody

Human Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms E, P
50 µg / €470.00
  Acris Antibodies GmbH

Collagen type VI antibody

Collagen type VI Rabbit Purified Bov, Chk, Hu, Ms, Por, Rt C, E, ICC/IF, P, R
0.5 ml / €600.00
  Acris Antibodies GmbH

Collagen type VI antibody

Collagen type VI Rabbit Purified Bov, Chk, Hu, Ms, Por, Rt C, E, ICC/IF, P, R
0.1 ml / €370.00
  Acris Antibodies GmbH

Collagen type VI antibody

Anti Collagen VI antibody (Cat.-No R1043 Lot 26009, 1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit IgG Purified Bov, Hu, Mam C, E, IP, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Collagen type VI antibody

Anti-Collagen VI Antibody (Cat.-No R1043 Lot 26009, 1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit IgG Biotin Bov, Hu, Mam C, E, IP, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Collagen type VI antibody

Anti Collagen VI antibody (Cat.-No R1043 Lot 26009, 1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA Rabbit IgG Purified Bov, Hu, Mam C, E, IP, P, WB
0.5 mg / €840.00
  Acris Antibodies GmbH

Collagen type VI antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Collagen VI alpha1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €230.00
  Assay Biotech

Collagen type VI antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Collagen VI alpha1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

COL6A1 - human gene knockout kit via CRISPR

COL6A1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

COL6A1 - human gene knockout kit via CRISPR

COL6A1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

COL6A1 - human gene knockout kit via CRISPR

COL6A1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

COL6A1 - human gene knockout kit via CRISPR

COL6A1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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