Complement C4B antibody

Principal name

Complement C4B antibody

Alternative names for Complement C4B antibody

Complement component 4, Basic complement C4, CO4, CPAMD3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

SwissProt ID

P01029 (Mouse), P0C0L5 (Human)

Gene ID

102643089 (LOC102643089), 100293534 (LOC100293534)

Available reactivities

Ms (Mouse), Hu (Human), Por (Porcine), Rt (Rat)

Available hosts

Rat, Rabbit, Mouse, Chicken

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Dot blot (Dot), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of Complement C4B antibody

In eukaryotic cells, DNA is associated with histones and other proteins to form chromatin. The cell division cycle constitutes a series of processes that have evolved to create two genetically identical daughter cells from a mother cell. One of these processes is the conversion of relatively amorphous, extended interphase chromatin into condensed, highly ordered mitotic chromosomes. Proper mitotic chromosome condensation is essential for the correct segregation of sister chromatids into two daughter cells. The basic unit of chromatin is the nucleosome core particle, which consists of 140 bp DNA wrapped around an octameric core containing two each of the four conserved core histones: H2A, H2B, H3 and H4. A fifth histone, the linker histone H1, interacts with DNA of variable length, linking adjacent nucleosome cores, and further compacting the chromatin. Chromatin changes are initiated during G2 phase of the cell cycle, in preparation for cell division. The most striking morphological change is chromatin condensation, which becomes apparent during prophase and is maximal during the subsequent stages of mitosis. Histone H1 and the N-terminal tail of H3 have key roles in the folding and inter-association of the chromatin fiber. Two currently known phosphorylation sites are present in the N-terminus of H3; serine-10 and serine-28.1,2 Mitogenic stimulation, oncogenic transformation, or induction of oncogenic ras expression are accompanied with increase in serine-10 phosphorylation of the H3 N-terminal domain. Indeed, it has been shown that phosphorylated H3 is associated with c-fos and c-myc genes in stimulated cells. Phosphorylation of H3, at both serine-10 and -28, coincides with the induction of mitotic chromosome condensation. H3 phosphorylation may contribute to proto-oncogene induction by modulating chromatin structure and releasing blocks in elongation. In contrast to H1 hyperphosphorylation, site-specific phosphorylation of core histone H3 at serine-10 and -28 appears to occur exclusively during mitosis in mammalian cells. H3 dephosphorylation occurs quite rapidly after mitosis and serine-10/28 remain unphosphorylated throughout the remainder of interphase. PP1 has been identified as the H3 phosphatase. Monoclonal antibodies reacting specifically with phosphorylated histone H3, are useful tools to study molecular mechanisms associated with the G2 to M transition and chromatin condensation, and for the analysis of protein kinase(s) and phosphatase(s) involved in H3 phosphorylation or dephosphorylation. They may also be used in multiparameter analysis to relate H3 phosphorylation in individual cells to the cell

General readings

Kremmer E et al. Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4. Hybridoma 9:309-17 (1990). PubMed PMID: 2210776

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Complement C4 (C4b+C4d) antibody

Complement C4 Rat IgG2a 16D2 Purified Ms C, E, ICC/IF, IP, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

Complement C4b antibody

Anti-C4B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. Chicken Ig Purified Hu E, F, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Complement C4B (C4d) antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with C4B antibody AP22740PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu P
0.25 ml / €470.00
  Acris Antibodies GmbH

Complement C4B antibody

Complement C4B Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Complement C4B antibody

Human HeLa; WB Suggested Anti-C4B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; C4B antibody - N-terminal region (ARP41841_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Complement C4B antibody

Complement C4B Rat IgG2a 16D2 Purified Ms C, ICC/IF, IP, P, WB
50 µg / €400.00
  Novus Biologicals Inc.

Complement C4B antibody

Western Blot: C4b Antibody [NBP1-31229] - Western blot analysis of C4b Antibody on: A:H1299 5% SDS PAGE diluted at 1:1000 Rabbit IgG Hu Dot, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Complement C4B antibody

Complement C4B Chicken Aff - Purified Hu E, F, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

Complement C4B antibody

Complement C4B Rabbit Aff - Purified Hu P
0.25 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Complement C4B

Complement C4B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Complement C4B

Complement C4B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Complement C4B

Complement C4B Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

Complement C4B

Complement C4B Mouse
10 µg / €370.00
  Abnova Taiwan Corp.

Complement C4B

Complement C4B Mouse
25 µg / €490.00
  Abnova Taiwan Corp.

14 Item(s)

per page
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