COPS5 antibody

Principal name

COPS5 antibody

Alternative names for COPS5 antibody

Signalosome subunit 5, SGN5, CSN5, JAB1, COP9 signalosome complex subunit 5, Jun activation domain-binding protein 1

SwissProt ID

O35864 (Mouse), Q6GLM9, Q6P635, Q6PC30, Q92905 (Human)

Gene ID

10987 (COPS5), 26754 (Cops5), 393698 (cops5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Whole mount Immunohistochemistry (IHC-wm)

Background of COPS5 antibody

Jun activation domain-binding protein 1 (JAB1) also designated COP9 subunit 5 (COPS5) or SGN5 is a coactivator of AP1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP1 containing complexes, and enhances transactivation from AP1 dependent promoters. It also interacts with Jun D but not with Jun B or v-Jun. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both the nucleus and the cytoplasm. It interacts with the cytoplasmic domain of the alphaL/beta2 integrin LFA1. Following LFA1 engagement the nuclear pool of JAB1 increases and activation of an AP1 driven promoter is enhanced. Interaction of JAB1 with the nuclear progesterone receptor and the steroid receptor activator (SRC1) was reported.JAB1 is a stability and activity regulator of Hypoxia inducible factor 1 (HIF1), a transcription factor that controls activation of several genes responsive to the cellular oxygen tension. The macrophage migration inhibitory factor (MIF) associates with JAB1 in the cytosol near the plasma membrane. Endogenous MIF inhibits JAB1 induced AP1 transcriptional activity. JAB1 is a subunit of the COP9 regulatory complex. COP9 cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. A metalloprotease motif in JAB1 plays a role in this isopeptidase activity. Breakdown of the cyclin dependent kinase inhibitor p27Kip1 is promoted by JAB1. The latter expression in several cancers inversely correlates with p27Kip1 and may reflect tumor aggressiveness. A possible involvement of JAB1 in atherosclerosis was also reported. Involvement of JAB1 in degradation of the suppressors p53 and smad4 was described recently.

General readings

Boussiotis VA et al. p27kip1 functions as an anergy factor inhibiting interleukin 2 transcription and clonal expansion of alloreactive human and mouse helper T lymphocytes. Nat Med 6:290-7 (2000). PubMed PMID: 10700231

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

COPS5 antibody

Western blot analysis of extracts of various cell lines, using COPS5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

COPS5 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504344) Mouse IgG1 OTI2B12 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

COPS5 (N-term) antibody

Human breast ca. stained with Anti-JAB1, Cat# AP15612PU. Rabbit Purified Hu, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

COPS5 (N-term) antibody

Human breast ca. stained with Anti-JAB1, Cat# AP15612PU. Rabbit Purified Hu, Ms, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

COPS5 (N-term) antibody

Human breast ca. stained with Anti-JAB1, Cat# AP15612PU. Rabbit Purified Hu, Ms, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

COPS5 antibody

Western blot analysis of extracts from 293 cells and K562 cells, using COPS5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

COPS5 antibody

Predicted band size: 38 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/1550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

COPS5 antibody

WB Suggested Anti-COPS5 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

COPS5 (full length) antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) anti JAB1 antibody (AM06040PU-N) Mouse IgG2b 2A10.8 Purified Hu, Ms ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

COPS5 (full length) antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG2a 6C3.38 Purified Hu, Ms IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

COPS5 (full length) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 8H8.5 Purified Hu, Ms IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

COPS5 antibody

Immunohistochemistry (IHC) analyzes of JAB1 antibody (Cat.-No.: AP20452PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

COPS5 (N-term) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

COPS5 antibody

COPS5 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

COPS5 antibody

Immunohistochemical staining of COPS5 on formalin fixed, paraffin embedded human breast cancer with COPS5 polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

COPS5 antibody

Western blot analysis of extracts from 293/K562 cells, using COPS5 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

COPS5 antibody

Western blot analysis of extracts from 293/K562 cells, using COPS5 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

COPS5 antibody

Mouse B8, Human HEK293; . Sample Type: 1. Human blood cells, pure. 2. Human blood cells, partially purified. 3. and 4. Human total cell extract, extraction only. Primary Dilution: 1:500. Secondary Anitbody: HRP conjugated anti-rabbit. Image Submitted By: Dr. Elah Pick. University of Haifa at Oranim .; COPS5 antibody - N-terminal region (ARP31512_P050) in Mouse B8, Human HEK293 cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

COPS5 antibody

Human Heart; COPS5 antibody - N-terminal region (ARP32834_P050) in Human Heart cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

COPS5 antibody

Immunohistochemical analysis of JAB1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Por, Rt, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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