Coronin-1A antibody

Principal name

Coronin-1A antibody

Alternative names for Coronin-1A antibody

Coronin-like protein p57, Coronin-like protein, CORO1A, CORO1, Clipin-A, TACO, Tryptophan aspartate-containing coat protein

SwissProt ID

O89053 (Mouse), P31146 (Human), Q91ZN1 (Rat), Q92176 (Bovin)

Gene ID

11151 (CORO1A)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rb (Rabbit), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, Fe (Feline), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Coronin-1A antibody

Coronin was originally discovered in Dictyostelium, where it was found to be involved in the chemotactic response of these ameboid cells. The name derives from the fact that the protein is localized at the leading edge or crown of these highly motile cells. The name derives from Corona, which is latin for crown. Coronin homologues have been found in yeast, C. elegans, Drosophila and many other species, and a family them are known in mammals. All coronins belong to the WD40 or WD family of proteins, the prototype or which is the beta subunit of trimeric G-proteins. The beta subunit proteins all have a beautiful conserved wheel-like seven bladed beta-propeller structure, with each blade being formed by four beta strands generated by one of the WD sequence repeats. The G protein beta subunits are believed to function as general purpose binding adapters, mediating numerous regulatory binding interactions between G proteins, G protein coupled receptors and G protein effectors. Although sequence analysis suggested that coronin 1a only possess 5 of the these WD repeats, recent structural studies show that, like the G protein beta-subunits, there are seven beta-propellers, generating a compact 7 bladed propellor. Coronins appear to be particularly involved in binding to actin, actin associated proteins, tubulin and phospholipase C and have been implicated in the mechanisms of chemotaxis and phagocytosis. In mammals there are at least five major coronin proteins, named coronins 1 to 5 in one nomenclature. Another nomenclature divides these five proteins in coronins 1a and 1b, 2a, 2b and 2c (see the HUGO (Human Genone Organization) Gene Nomenclature Committee link for this family). The various coronin proteins have several other alternate names, since they were discovered independently by several different groups. The mammalian coronin family members are abundant components of eukaryotic cells, and each type has a restricted cell type specific expression pattern. Coronin 1A is the mammalian coronin most similar in protein sequence to the Dictyostelium protein and is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils. NB 110-58867 is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils. Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type. Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors. Since coronin 1a is a constitutive component of microglia, the coronin 1a antibody can be used to study both quiescent and activated microglia.

General readings

Ferrari G et al. A coat protein on phagosomes involved in the intracellular survival of mycobacteria. Cell 97:435-47 (1999). PubMed PMID: 10338208

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Coronin-1A antibody

Anti-CORO1A mouse monoclonal antibody (TA501429) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CORO1A(RC210753 ). Mouse IgG1 OTI1A5 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Coronin-1A (C-term) antibody

TA302455 staining (0.3µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Coronin-1A antibody

Western Blot: Coronin 1a Antibody - Western blot of HL60 Cell extract stained with RPCA-Cor1a, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~57kDa corresponds to Coronin 1a. Numbers represent positions of molecular weight standards in kDa. Rabbit Purified Hu, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Coronin-1A antibody

Western blot analysis of extracts from Jurkat cells, using CORO1A antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Coronin-1A antibody

WB Suggested Anti-CORO1A Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate; CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Coronin-1A antibody

WB Suggested Anti-CORO1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Daudi cell lysate; CORO1A is supported by BioGPS gene expression data to be expressed in Daudi Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

Coronin-1A antibody

WB Suggested Anti-CORO1A Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate; CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Coronin-1A antibody

WB Suggested Anti-CORO1A Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate; CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Coronin-1A antibody

Coronin-1A Rabbit IgG Liquid Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Coronin-1A (546-558) antibody

Immunohistochemistry staining of human spleen (Formalin-fixed, Paraffin-embedded tissue after heat-induced antigen retrieval) using Coronin-1A antibody Cat.-No.AP22532PU-N (concentration 75 ug/ml).
  Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mink, Ms, Prim, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Coronin-1A antibody

Dissociated cell culture from a neonatal Mouse brain showing Coronin 1a (green staining) of microglial cells. These cultures were counter-stained with Rabbit antibodies against NFM (neurofilament, red staining), a neuronal marker, as well as DAPI (blue staining), a marker of nuclei. Picture courtesy of Dr. Gerry Shaw, University of Florida. Chicken Ig Aff - Purified Hu, Ms ICC/IF, P
0.1 mg / €440.00
  Acris Antibodies GmbH

Coronin-1A antibody

Coronin-1A Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Coronin-1A antibody

Coronin-1A Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

Coronin-1A antibody

Immunoperoxidase of monoclonal antibody to CORO1A on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml] Mouse IgG2a 4G10 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Coronin-1A antibody

Coronin-1A Mouse IgG2a 1A8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Coronin-1A antibody

CORO1A polyclonal antibody ( Cat # PAB6460 ) staining ( 0.3 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Coronin-1A antibody

Immunofluorescence of a mixed neuron/glial culture from newborn rat brain stained with CORO1A polyclonal antibody ( Cat # PAB12563 ) ( green ) at a dilution of 1 : 10000. Rabbit IgG Hu, Rt ICC/IF, WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

Coronin-1A antibody

Immunocytochemistry of a mixed neuron/glial culture from newborn rat brain stained with CORO1A polyclonal antibody ( Cat # PAB14169 ) ( green ) at a dilution of 1 : 10,000 and Chicken Vimentin polyclonal antibody (1 : 5,000 ).
Blue is nuclear DNA counter stain.
Glial cells and fibroblasts stain with Vimentin, while microglia alone stain strongly and specifically for CORO1A, which can therefore be used as a robust marker of this important cell type. Rabbit Hu C, E, ICC/IF, WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

Coronin-1A antibody

Human Jurkat; WB Suggested Anti-CORO1A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate. CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat.; CORO1A antibody - C-terminal region (ARP34285_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

Coronin-1A antibody

Human Daudi; WB Suggested Anti-CORO1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysate. CORO1A is supported by BioGPS gene expression data to be expressed in Daudi.; CORO1A antibody - middle region (ARP34286_P050) in Human Daudi cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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