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Coronin-1A antibody

Principal name

Coronin-1A antibody

Alternative names for Coronin-1A antibody

Coronin-like protein p57, Coronin-like protein, CORO1A, CORO1, Clipin-A, TACO, Tryptophan aspartate-containing coat protein

SwissProt ID

O89053 (Mouse), P31146 (Human), Q91ZN1 (Rat), Q92176 (Bovin)

Gene ID

11151 (CORO1A), 12721 (Coro1a), 155151

Ncbi ID

9606, NP_009005, NP_001180262, ABZ92302.1, NP_001180262.1, NP_009005.1, NP_034028.1, NM_130411, NM_007074

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rb (Rabbit), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, Fe (Feline), Bt (Bat), Eq (Equine), Hst (Hamster), Mink, Prim (Primate), Bear, GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit, Chicken, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of Coronin-1A antibody

Kit Component:: - KN303702G1, Coro1a gRNA vector 1 in pCas-Guide vector
- KN303702G2, Coro1a gRNA vector 2 in pCas-Guide vector
- KN303702D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Anand,P.K., et al., (2005) FEMS Microbiol. Lett. 250 (1), 137-144

Immunoperoxidase of monoclonal antibody to CORO1A on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]

H00011151-M01

CORO1A monoclonal antibody (M01), clone 4G10 Western Blot analysis of CORO1A expression in K-562 ( Cat # L009V1 ).

H00011151-M01

Detection limit for recombinant GST tagged CORO1A is approximately 0.1ng/ml as a capture antibody.

H00011151-M01

Human Jurkat; WB Suggested Anti-CORO1A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat.; CORO1A antibody - C-terminal region (ARP38900_T100) in Human Jurkat cells using Western Blot

ARP38900_T100

Human Jurkat; WB Suggested Anti-CORO1A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate. CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat.; CORO1A antibody - C-terminal region (ARP34285_T100) in Human Jurkat cells using Western Blot

ARP34285_T100

Human Daudi; WB Suggested Anti-CORO1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysate. CORO1A is supported by BioGPS gene expression data to be expressed in Daudi.; CORO1A antibody - middle region (ARP34286_P050) in Human Daudi cells using Western Blot

ARP34286_P050

Human Jurkat; WB Suggested Anti-CORO1A Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat.; CORO1A antibody - N-terminal region (ARP38899_T100) in Human Jurkat cells using Western Blot

ARP38899_T100

CORO1A polyclonal antibody ( Cat # PAB6460 ) staining ( 0.3 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PAB6460

Immunofluorescence of a mixed neuron/glial culture from newborn rat brain stained with CORO1A polyclonal antibody ( Cat # PAB12563 ) ( green ) at a dilution of 1 : 10000.

PAB12563

Western blot of CORO1A in HL-60 Cell extract stained with RPCA-Cor1a CORO1A polyclonal antibody ( Cat # PAB12563 ) at dilution of 1 : 10000.

PAB12563

Immunocytochemistry of a mixed neuron/glial culture from newborn rat brain stained with CORO1A polyclonal antibody ( Cat # PAB14169 ) ( green ) at a dilution of 1 : 10,000 and Chicken Vimentin polyclonal antibody (1 : 5,000 ).Blue is nuclear DNA counter stain.Glial cells and fibroblasts stain with Vimentin, while microglia alone stain strongly and specifically for CORO1A, which can therefore be used as a robust marker of this important cell type.

PAB14169

Western blot of HL-60 cell extracts stained with CORO1A polyclonal antibody ( Cat # PAB14169 ), at dilution of 1 : 10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~ 57kDa corresponds to CORO1A.Numbers represent positions of molecular weight standards in kDa.

PAB14169

Immunohistochemistry staining of human spleen (Formalin-fixed, Paraffin-embedded tissue after heat-induced antigen retrieval) using Coronin-1A antibody Cat.-No.AP22532PU-N (concentration 75 ug/ml).

AP22532PU-N

Immunohistochemistry staining of human small intestine (Formalin-fixed, Paraffin-embedded tissue after heat-induced antigen retrieval) using Coronin-1A antibody Cat.-No.AP22532PU-N (concentration 75 ug/ml).

AP22532PU-N

Western blot (using chemiluminescence) of Jurkat lysate (RIPA buffer, 35 µg total protein per lane, primary incubated for 1 hour) using Coronin-1A antibody Cat.-No. AP22532PU-N (C-term).

AP22532PU-N

Dissociated cell culture from a neonatal Mouse brain showing Coronin 1a (green staining) of microglial cells. These cultures were counter-stained with Rabbit antibodies against NFM (neurofilament, red staining), a neuronal marker, as well as DAPI (blue staining), a marker of nuclei. Picture courtesy of Dr. Gerry Shaw, University of Florida.

AP31804PU-N

Anti-CORO1A mouse monoclonal antibody (TA501429) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CORO1A(RC210753 ).

TA501429

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CORO1A (RC210753, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CORO1A.

TA501429

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CORO1A monoclonal antibody.

TA501429

Flow cytometric Analysis of Hela cells, using anti-CORO1A antibody(TA501429),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

TA501429

Flow cytometric Analysis of Jurkat cells, using anti-CORO1A antibody(TA501429),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

TA501429

HEK293T cells transfected with either RC210753 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CORO1A antibody(TA501429), and then analyzed by flow cytometry.

TA501429

TP310753

TA302455 staining (0.3µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TA302455

Western Blot: Coronin 1a Antibody - Western blot of HL60 Cell extract stained with RPCA-Cor1a, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~57kDa corresponds to Coronin 1a. Numbers represent positions of molecular weight standards in kDa.

TA309615

Immunocytochemistry: Coronin 1a Antibody - Immunocytochemistry of a mixed neuron/glial culture from newborn rat brain stained with anti-Coronin 1a (green) at a dilution of 1:10,000 and anti-Vimentin (1:5,000). Blue is nuclear DNA counter stain. Glial cells and fibroblasts stain with vimentin, while microglia alone stain strongly and specifically for Coronin 1a, which can therefore be used as a robust marker of this important cell type.

TA309615

Western blot analysis of extracts from Jurkat cells, using CORO1A antibody.The lane on the right is treated with the synthesized peptide.

TA326236

WB Suggested Anti-CORO1A Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate; CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat

TA335667

WB Suggested Anti-CORO1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Daudi cell lysate; CORO1A is supported by BioGPS gene expression data to be expressed in Daudi

TA335668

WB Suggested Anti-CORO1A Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate; CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat

TA343655

WB Suggested Anti-CORO1A Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate; CORO1A is supported by BioGPS gene expression data to be expressed in Jurkat

TA343656

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA501429	Coronin-1A antibody
	CORO1A (Coronin 1a) mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)
	Mouse	IgG1	OTI1A5	Purified	Hu	F, ICC/IF, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+5 additional images	Highly validated antibody!
TA309615	Coronin-1A antibody
	Rabbit Polyclonal Coronin-1a Antibody
	Rabbit			Purified	Hu, Rt	ICC/IF, WB	0.1 ml / €416.00
							OriGene Technologies, Inc.
+1 additional image
TA326236	Coronin-1A antibody
	Rabbit polyclonal CORO1A antibody
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA335667	Coronin-1A antibody
	Rabbit Polyclonal Anti-CORO1A Antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Rb, Rt	WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA335668	Coronin-1A antibody
	Rabbit Polyclonal Anti-CORO1A Antibody
	Rabbit	IgG		Purified	Can, Eq, GP, Hu, Ms, Por, Rb	WB	50 µg / €360.00
							OriGene Technologies, Inc.
TA343655	Coronin-1A antibody
	Rabbit Polyclonal Anti-CORO1A Antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze	WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA343656	Coronin-1A antibody
	Rabbit Polyclonal Anti-CORO1A Antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, Hu, Ms, Por, Rb, Rt	WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
CH23017-100	Coronin-1A antibody
	Chicken			Purified	Hu, Ms	C, ICC/IF	0.1 ml / €180.00
							Neuromics Antibodies

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