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Cortactin antibody

Principal name

Cortactin antibody

Alternative names for Cortactin antibody

CTTN, EMS1, Amplaxin, Oncogene EMS1

SwissProt ID

Q01406 (Chick), Q14247 (Human), Q60598 (Mouse)

Gene ID

2017 (CTTN), 13043 (Cttn)

Ncbi ID

10090, 9606, NP_005222, NP_005222.2, NP_612632, BC011434, NM_005231

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Cortactin antibody

CTTN is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. CTTN is localized in the cytoplasm and in areas of the cell-substratum contacts. It has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, CTTN is degraded in a caspase-dependent manner. The aberrant regulation of t CTTN contributes to tumor cell invasion and metastasis.This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

General readings

1. In J et al. Serine protease EspP from enterohemorrhagic Escherichia Coli is sufficient to induce shiga toxin macropinocytosis in intestinal epithelium. PLoS One 8:e69196 (2013).
2. Sheen-Chen SM et al. Cortactin in breast cancer: analysis with tissue microarray. Anticancer Res 31:293-7 (2011). IHC-P ; Human .
3. Miyaso H et al. The changes of cortactin p80/85 isoform profiles and tyrosine phosphorylation status during spermatogenesis in the mouse testis. J Androl 31:507-18 (2010).
4. Nieto-Pelegrin E & Martinez-Quiles N Distinct phosphorylation requirements regulate cortactin activation by TirEPEC and its binding to N-WASP. Cell Commun Signal 7:11 (2009). WB ; Mouse .

Western blot analysis of extracts from Hela cells, untreated or treated with UV (20min), using Cortactin antibody (Lane 1 and 2) and Cortactin (PTyr466) antibody (AP02517PU-S; AP02517PU-N, Lane 3 and 4).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Cortactin antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Cortactin antibody.

Western blot analysis of extracts from Hela cells, untreated or treated with UV (20min), using Cortactin antibody (Lane 1 and 2) and Cortactin (PTyr466) antibody (Lane 3 and 4).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cttn polyclonal antibody ( Cat # PAB5878 ) .

PAB5878

CTTN polyclonal antibody ( Cat # PAB6083 ) staining ( 0.5 µg/ml ) of A-431 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PAB6083

Proximity Ligation Analysis (PLA) of protein-protein interactions between SYK and CTTN. HeLa cells were stained with anti-SYK rabbit purified polyclonal 1:1200 and anti-CTTN mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00002017-M01

CTTN MaxPab polyclonal antibody. Western Blot analysis of CTTN expression in rat brain.

H00002017-B01

Western Blot analysis of CTTN expression in transfected 293T cell line (H00002017-T01) by CTTN MaxPab polyclonal antibody.Lane 1: CTTN transfected lysate(56.43 KDa).Lane 2: Non-transfected lysate.

H00002017-B01

Hum. Fetal Liver; Host:Rabbit. Target Name:CTTN. Sample Tissue:Human Fetal Liver. Antibody Dilution: 1.0ug/ml.; CTTN antibody - N-terminal region (ARP52067_P050) in Hum. Fetal Liver cells using Western Blot

ARP52067_P050

Hum. Fetal Lung; Host:Rabbit. Target Name:CTTN. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; CTTN antibody - N-terminal region (ARP52067_P050) in Hum. Fetal Lung cells using Western Blot

ARP52067_P050

Hum. Fetal Muscle; Host:Rabbit. Target Name:CTTN. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; CTTN antibody - N-terminal region (ARP52067_P050) in Hum. Fetal Muscle cells using Western Blot

ARP52067_P050

Human Brain; WB Suggested Anti-CTTN Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; CTTN antibody - N-terminal region (ARP52067_P050) in Human Brain cells using Western Blot

ARP52067_P050

Western Blot analysis of CTTN expression in transfected 293T cell line (H00002017-T01) by CTTN MaxPab polyclonal antibody.Lane 1: CTTN transfected lysate(57.50 KDa).Lane 2: Non-transfected lysate.

H00002017-D01P

Immunoblot analysis of cell lysates.Lane 1 : HeLa cell lysate. Lnae 2 : C6 cell lysate.Lane 3 : COS-1 cell lysate.Lane 4 : SK-N-SH cell lysate.

PAB12809

Immunohistochemistry analyzes of Cortactin antibody (Cat.-No.: AP20737PU-N) in paraffin-embedded human prostate carcinoma tissue.

AP20737PU-N

Western blot analyzes of Cortactin antibody (Cat.-No.: AP20737PU-N) in extracts from HeLa cells.

AP20737PU-N

Immunohistochemistry analysis of Cortactin Antibody (Cat.-No AP20738PU-N) in paraffin-embedded human breast carcinoma tissue.

AP20738PU-N

Western blot analysis of Cortactin Antibody (Cat.-No AP20738PU-N) in extracts from HeLa cells.

AP20738PU-N

Immunohistochemistry analysis of Cortactin antibody (Cat.-No AP20857PU-N) in paraffin-embedded human prostate carcinoma tissue.

AP20857PU-N

Western blot analysis of Cortactin antibody (Cat.-No AP20857PU-N) in extracts from 3T3 H2O2 cells

AP20857PU-N

Western blot analysis of extracts from HeLa cells treated with H2O2, using Cortactin (Phospho-Tyr421) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cortactin (Phospho-Tyr466) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells, using Cortactin (Phospho-Tyr466) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, treated with H2O2, using Cortactin (Ab-421) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Cortactin (Ab-466) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from LOVO cells, using Cortactin (Ab-466) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of human breast carcinoma (Formalin-fixed, Paraffin-embedded) using Cortactin (SRC8) antibody Cat.-No. AP54041PU-N (N-term), followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC; Clinical relevance has not been evaluated.

AP54041PU-N

Western blot analysis of Cortactin (arrow) in HL-60 cell line lysates (35ug/lane) using Cortactin (SRC8) antibody Cat.-No. AP54041PU-N (N-term).

AP54041PU-N

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and tonsil tissues (right) using CTTN antibody Cat.-No AM06601SU-N with DAB staining.

AM06601SU-N

Western blot analysis using CTTN antibody Cat.-No AM06601SU-N against Hela (Lane 1), A431 (Lane 2), MCF-7 (Lane 3), SR-BR-3 (Lane 4), HepG2 (Lane 5) and NIH/3T3 (Lane 6) cell lysate.

AM06601SU-N

AM06601SU-N

Immunohistochemical analysis of Cortactin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Cortactin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of Cortactin expression in MCF7 (A); C6 (B); A431 (C); HeLa (D) whole cell lysates.

Western blot analysis of Cortactin expression in MCF7 (A); HeLa (B); NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of Cortactin (pY421) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of Cortactin (pY421) expression in HeLa UV-treated (A); NIH3T3 H2O2-treated (B) whole cell lysates.

Immunohistochemical analysis of Cortactin (pY466) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Cortactin (pY466) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of Cortactin (pY466) expression in HeLa UV-treated (A); NIH3T3 H2O2-treated (B) whole cell lysates.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CTTN Rabbit Polyclonal antibody. (TA590298)

TA590298

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTTN (RC201111, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTTN.

TA590298

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTTN polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

TA590298

ELISA: Cortactin Antibody

TA590298

TP301111

TP710315

TA302747 staining (0.5µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TA302747

Predicted band size: 85 kDa . Positive control: 3T3 and C2C12 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: 3T3 and C2C12 cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA324050

Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using CTTN (Ab-466) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

TA324050

Western blot analysis of Cortactin expression in H2O2 treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325399

Western blot analysis of Cortactin expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325400

Western blot analysis of extracts of various cell lines, using CTTN antibody.

TA332919

Immunofluorescence analysis of MCF-7 cell using CTTN antibody.

TA332919

Predicted band size: 85 kDa . Positive control: Jurkat cells treated with H2O2 lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Jurkat cells untreated with H2O2 lysate Lane2: Jurkat cells treated with H2O2 lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA324049

Western blot analysis of extracts from 293 cells using Cortactin (phospho-Tyr466) antibody.The lane on the right is treated with the synthesized peptide.

TA312466

Western blot analysis of extracts from COS7 cells (Lane 2) and JK cells (Lane 3), using Cortactin (Phospho-Tyr466) Antibody. The lane on the left is treated with synthesized peptide.

TA312466

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Cortactin (phospho-Tyr466) antibody.The picture on the right is treated with the synthesized peptide.

TA312466

Western blot analysis of extracts from HeLa cells untreated or treated with UV (20min), using Cortactin antibody (Lanes 1 and 2) and Cortactin (phospho-Tyr466) antibody (Lanes 3 and 4).

TA310011

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA302747	Cortactin (N-term) antibody
	Goat Polyclonal Antibody against CTTN
	Goat			Aff - Purified	Bov, Can, Hu, Ms, Rt	WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA310011	Cortactin antibody
	Rabbit anti-CTTN (Cortactin, Phospho-Tyr466) polyclonal antibody(Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 mg / €353.00
							OriGene Technologies, Inc.
TA312466	Cortactin antibody
	Rabbit polyclonal Cortactin (Tyr466) antibody(Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA324049	Cortactin antibody
	Anti-CTTN (Phospho-Tyr466) Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA324050	Cortactin antibody
	Anti-CTTN Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA325399	Cortactin antibody
	Rabbit Polyclonal Cortactin Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA325400	Cortactin antibody
	Rabbit Polyclonal Cortactin Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA332919	Cortactin antibody
	Rabbit anti-CTTN Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+1 additional image
AM06601SU-N	Cortactin antibody
	Mouse	IgG1	4C6	Ascites	Hu, Ms	E, P, WB	0.1 ml / €430.00
							Acris Antibodies GmbH
+2 additional images
AP02517PU-N	Cortactin pTyr466 antibody
	Rabbit			Aff - Purified	Ms	WB	0.1 mg / €320.00
							Acris Antibodies GmbH
AP02517PU-S	Cortactin pTyr466 antibody
	Rabbit			Aff - Purified	Ms	WB	50 µg / €240.00
							Acris Antibodies GmbH
AP02764PU-N	Cortactin antibody
	Rabbit			Aff - Purified	Ms	P	0.1 mg / €320.00
							Acris Antibodies GmbH
AP02764PU-S	Cortactin antibody
	Rabbit			Aff - Purified	Ms	P	50 µg / €240.00
							Acris Antibodies GmbH
AP02765PU-N	Cortactin antibody
	Rabbit			Aff - Purified	Ms	P, WB	0.1 mg / €320.00
							Acris Antibodies GmbH
+1 additional image
AP02765PU-S	Cortactin antibody
	Rabbit			Aff - Purified	Ms	P, WB	50 µg / €240.00
							Acris Antibodies GmbH
+1 additional image
AP20737PU-N	Cortactin antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
AP20738PU-N	Cortactin antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
AP20857PU-N	Cortactin pTyr421 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
AP54041PU-N	Cortactin (N-term) antibody
	Rabbit	Ig		Aff - Purified	Hu	P, WB	0.4 ml / €370.00
							Acris Antibodies GmbH
+1 additional image
A7049-1	Cortactin pTyr421 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	E, WB	50 µg / €230.00
							Assay Biotech