COX17 antibody
Principal name
COX17 antibody
Alternative names for COX17 antibody
COX-17, Cytochrome c oxidase copper chaperone
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of COX17 antibody
Cytochrome c oxidase (COX), the termil component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be involved in the recruitment of copper to mitochondria for incorporation into the COX apoenzyme. This protein shares 92% amino acid sequence identity with mouse and rat Cox17 proteins. This gene is no longer considered to be a candidate gene for COX deficiency. A pseudogene COX17P has been found on chromosome 13.
Immunoperoxidase of monoclonal antibody to COX17 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]
COX17 monoclonal antibody (M01), clone 4G2. Western Blot analysis of COX17 expression in IMR-32.
Detection limit for recombinant GST tagged COX17 is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged COX17 is approximately 0.03ng/ml as a capture antibody.
Immunohistochemistry (IHC) analyzes of COX17 antibody (Cat.-No.: AP20541PU-N) in paraffin-embedded human liver carcinoma tissue.
Western blot (WB) analysis of COX17 antibody (Cat.-No.: AP20541PU-N) in extracts from HeLa cells.
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
COX17 monoclonal antibody clone 4G2. Western Blot analysis of COX17 expression in IMR-32.
Detection limit for recombinant GST tagged COX17 is approximately 0.03 ng/ml as a capture antibody.
Immunohistochemical analysis of COX17 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of COX17 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of COX17 expression in IMR32 (A); HepG2 (B) whole cell lysates.
Western blot analysis of extracts from HeLa cells, HepG2 cells, COLO cells and 293 cells, using COX17 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 3T3 cells (Lane 2), using COX17 Antibody The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using COX17 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis on HeLa cell lysate using COX17 Antibody
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM20978PU-N | COX17 antibody |
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Mouse | IgG2b | 4G2 | Hu | E, P, WB |
50 µg /
€420.00
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| Acris Antibodies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA315013 | COX17 antibody |
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| Rabbit polyclonal anti-COX17 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA347704 | COX17 antibody |
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| Rabbit Polyclonal Anti-COX17 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP20541PU-N | COX17 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€350.00
|
||
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| C12231-1 | COX17 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µg /
€230.00
|
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| Assay Biotech | |||||||
| +1 additional image |
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| C12231-2 | COX17 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€330.00
|
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| Assay Biotech | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN210756 | COX17 - human gene knockout kit via CRISPR |
||
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN210756BN | COX17 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN210756LP | COX17 - human gene knockout kit via CRISPR |
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|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN210756RB | COX17 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC210756L2V | Lenti ORF particles, COX17 (mGFP-tagged)-Human COX17 cytochrome c oxidase assembly homolog (S. cerevisiae) (COX17), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| RC210756L1V | Lenti ORF particles, COX17 (Myc-DDK-tagged)-Human COX17 cytochrome c oxidase assembly homolog (S. cerevisiae) (COX17), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR200043L2V | Lenti ORF particles, Cox17 (GFP-tagged) - Mouse cytochrome c oxidase, subunit XVII assembly protein homolog (yeast) (Cox17), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| MR200043L1V | Lenti ORF particles, Cox17 (Myc-DDK-tagged) - Mouse cytochrome c oxidase, subunit XVII assembly protein homolog (yeast) (Cox17), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
