CpG-binding protein antibody

Principal name

CpG-binding protein antibody

Alternative names for CpG-binding protein antibody

CXXC1, CFP1, CGBP, PCCX1, PHF18, PHD finger and CXXC domain-containing protein 1, CXXC-type zinc finger protein 1

SwissProt ID

Q5EA28 (Bovin), Q9CWW7 (Mouse), Q9P0U4 (Human)

Gene ID

30827 (CXXC1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of CpG-binding protein antibody

Human CpG binding protein (hCGBP) contains three cysteine-rich domains, two of which
exhibit homology to the plant homeodomain finger domain and the third cysteine-rich
domain conforms to the CXXC motif identified in DNA methyltransferase, human trithorax,
and methyl-CpG binding domain protein 1. A fragment of hCGBP that contains the CXXC
domain binds to an oligonucleotide probe containing a single CpG site, and this complex is
disrupted by distinct oligonucleotide competitors that also contain a CpG motif(s). CBGP
plays an important part in embryonic viability and peri-implantation development. Native
hCGBP is detected as an 88-kDa protein by Western analysis and is ubiquitously
expressed. CGBP trans-activates promoters that contain CpG motifs but not promoters in
which the CpG is ablated suggesting that hCGBP is a transcriptional activator that
recognizes unmethylated CpG dinucleotides.

General readings

Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)
Tate, C.M., et al. Mol. Cell. Biol. 29(14):3817-3831(2009)
Butler, J.S., et al. DNA Cell Biol. 27(10):533-543(2008)
Ansari, K.I., et al. Biochim. Biophys. Acta 1779(1):66-73(2008)
Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CpG-binding protein antibody

WB Suggested Anti-CXXC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CpG-binding protein antibody

WB Suggested Anti-Cxxc1 Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Testis Rabbit IgG Purified Bov, Can, Eq, Gt, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CpG-binding protein antibody

Western blot analysis of extracts of various cell lines, using CXXC1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CpG-binding protein antibody

WB Suggested Anti-CXXC1 Antibody Titration: 0.625 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CpG-binding protein (Center) antibody

Wwestern blot analysis in mouse liver tissue lysates (35ug/lane) using CpG-binding protein/CXXC1 antibody (Cat.-No. AP51152PU-N).This demonstrates the CXXC1 antibody detected the CXXC1 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CpG-binding protein antibody

Western blot analysis of CXXXC1. Using CXXC1 polyclonal antibody ( Cat # PAB0206 ) at 1 : 1,000 dilution against 15 ug/lane of human spleen lysate. Immunoreactivity was determined using SuperSignal system ( Pierce ) and by exposing to the film for 15 minutes. Rabbit Hu WB
0.2 ml / €400.00
  Abnova Taiwan Corp.

CpG-binding protein antibody

Human THP-1; WB Suggested Anti-CXXC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; CXXC1 antibody - middle region (ARP32621_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CpG-binding protein antibody

Transfected 293T; WB Suggested Anti-CXXC1 Antibody Titration: 0.625ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; CXXC1 antibody - C-terminal region (ARP39110_T100) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

CpG-binding protein antibody

Western Blot: CXXC1 Antibody [NBP1-71916]- Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells.  Affinity purified rabbit anti-CFP1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  CFP1 was also immunoprecipitated by rabbit anti-CFP1 antibody NBP1-71917, which recognizes a downstream epitope. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CpG-binding protein antibody

Western Blot: CXXC1 Antibody [NBP1-71917]-Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.   Affinity purified rabbit anti-CFP1 antibody used for IP at 6 mcg/mg lysate.  CFP1 was also immunoprecipitated by rabbit anti-CFP1 antibody NBP1-71916, which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

CpG-binding protein antibody

Immunohistochemistry-Paraffin: CXXC1 Antibody [NBP1-91816] Immunohistochemical staining of human stomach shows nuclear and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

CXXC1 overexpression lysate

CXXC1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

12 Item(s)

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