CRABP2 antibody

Principal name

CRABP2 antibody

Alternative names for CRABP2 antibody

CRABP-II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid-binding protein II

SwissProt ID

P22935 (Mouse), P29373 (Human), P30370 (Chick), P51673 (Rat), Q5PXY7 (Bovin)

Gene ID

1382 (CRABP2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of CRABP2 antibody

The cellular retinoic acid-binding protein (CRABP)-I and a related isoform CRABP-II are nuclear receptors for retinoic acid (RA), an important regulator of cell growth and differentiation in fetal and adult tissues. These CRABP proteins mediate the downstream effects of RA in distinct ways. CRABP-I negatively regulates the activity of RA by enhancing the production of RAmetabolizing enzymes and increasing the rate at which RA is degraded. CRABP-II enhances the effects of RA by directly interacting with RA receptors (RAR) and, in turn, promoting the formation of RAR-RA complexes and stimulating RA-mediated gene transcription. Both CRABP-I and CRABP-II are expressed in the embryo, and CRABP-I is ubiquitously expressed in various adult tissues. The expression of CRABP-II is elevated in cells that synthesize relatively large amounts of RA, and it is also predominantly expressed in skin, uterus, ovary, and in the choroid plexus.

General readings

1. Yao PL  et al. Activation of peroxisome proliferator-activated receptor-ß/d (PPAR-ß/d) inhibits human breast cancer cell line tumorigenicity. Mol Cancer Ther 13:1008-17 (2014). Human .
2. Borland MG  et al. Stable over-expression of PPARß/d and PPAR? to examine receptor signaling in human HaCaT keratinocytes. Cell Signal 23:2039-50 (2011). WB ; Human . 

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CRABP2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRABP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800069) Mouse IgG1 OTI10D6 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CRABP2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CRABP2 mouse monoclonal antibody. (UM800069; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB176 Purified Hu P
0.1 ml / €379.00
  OriGene Technologies, Inc.

UltraMAB

CRABP2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CRABP2 mouse monoclonal antibody. (UM800069; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB176 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CRABP2 antibody

Western blot analysis of extracts from HT-29 cells, using CRABP2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRABP2 antibody

Predicted band size: 16 kDa . Positive control: MCF-7 and HT-29 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1,2: MCF-7 and HT-29 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRABP2 antibody

Host: Rabbit; Target Name: CRABP2; Sample Tissue: Hela Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRABP2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRABP2 (RC200221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRABP2. Mouse IgG1 OTI3C11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRABP2 antibody

Immunohistochemistry: AM09062PU-N CRABP2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Uterus at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a AT2E11 Purified Hu, Ms E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CRABP2 antibody

Immunohistochemistry: AM09062PU-N CRABP2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Uterus at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a AT2E11 Purified Hu, Ms E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

CRABP2 antibody

Western blot (WB) analysis of CRABP-II antibody (Cat.-No.: AP01466PU-N) in extracts from RAW264.7 cell Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CRABP2 (C-term) antibody

Western blot analysis of CRABP2 (arrow) using CRABP2 Antibody (C-term) Cat.-No AP51069PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CRABP2 gene. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRABP2 antibody

Immunofluorescence of purified MaxPab antibody to CRABP2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CRABP2 antibody

Western Blot analysis of CRABP2 expression in transfected 293T cell line (H00001382-T01) by CRABP2 MaxPab polyclonal antibody.

Lane 1: CRABP2 transfected lysate(15.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CRABP2 antibody

CRABP2 Mouse IgG2a 4F2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CRABP2 antibody

Western blot analysis of extracts from HT-29 cells, using CRABP2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CRABP2 antibody

Western blot analysis of extracts from HT-29 cells, using CRABP2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CRABP2 (Center) antibody

Human PANC1; WB Suggested Anti-CRABP2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: PANC1 cell lysate. CRABP2 is supported by BioGPS gene expression data to be expressed in PANC1.; CRABP2 antibody - middle region (ARP41982_P050) in Human PANC1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRABP2 antibody

Human 721_B; WB Suggested Anti-CRABP2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate; CRABP2 antibody - middle region (ARP41983_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CRABP2 antibody

Immunohistochemical analysis of CRABP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CRABP2 antibody

Immunohistochemical analysis of CRABP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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