Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of CREB-binding protein antibody
This gene is ubiquitously expressed and is involved in the transcriptiol coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB); this gene is now known to play critical roles in embryonic development; growth control; and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additiol protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain; cysteine-histidine-rich regions; and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Altertive splicing results in multiple transcript variants encoding different isoforms.
Proximity Ligation Analysis (PLA) of protein-protein interactions between FGFR1 and CREBBP HeLa cells were stained with anti-FGFR1 rabbit purified polyclonal 1:1200 and anti-CREBBP mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Human Breast; Anti-CREBBP antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.; CREBBP antibody - N-terminal region (ARP43609_P050) in Human Breast cells using Immunohistochemistry
Human Colon; Rabbit Anti-CREBBP Antibody. Catalog Number: arp43609. Paraffin Embedded Tissue: Human Colon. Antibody Concentration: 5 ug/ml; CREBBP antibody - N-terminal region (ARP43609_P050) in Human Colon cells using Immunohistochemistry
Human Prostate (formalin-fixed, paraffin-embedded) stained with CREBBP antibody AP22727PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Breast (formalin-fixed, paraffin-embedded) stained with CREBBP antibody AP22727PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemical analysis of CBP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CBP staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Predicted cell location: Nucleus . Positive control: Human cervical and esophagus cancer tissue. Recommended dilution: 1/5-20 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CREBBP antibody at dilution 1/5, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Nucleus . Positive control: Human cervical and esophagus cancer tissue. Recommended dilution: 1/5-20 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CREBBP antibody at dilution 1/5, on the right is treated with the synthetic peptide. (Original magnification: x200)