CREB1 antibody

Principal name

CREB1 antibody

Alternative names for CREB1 antibody

CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1

SwissProt ID

P15337 (Rat), P16220 (Human), P27925 (Bovin), Q01147 (Mouse)

Gene ID

1385 (CREB1), 81646 (Creb1)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Mky (Monkey), Sh (Sheep), Xen (Xenopus), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), Paraffin Sections (P), Electrophoretic Mobility Shift Assay (EMSA), ELISA (detection) (E(detection)), PLA/Proximity ligation assay (PLA), Dot blot (Dot), Frozen Sections (C)

Background of CREB1 antibody

CREB1 is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CREB1 with an internal ID of P00938

General readings

1. Johannessen M, Delghandi MP, Moens U (2004) What turns CREB on? Cellular Signalling 16:1211-1227.
2. Karin M, Hunter T (1995) Transcriptional control by protein phosphorylation: Signal transmission from the cell surface
to the nucleus. Curr Biol 5:747-757.
3. Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia ZG, Greenberg ME (2002) CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 34:221-233.
4. Montminy M (1997) Transcriptional regulation by cyclic AMP. Annu Rev Biochem 66:807-822.
5. Shaywitz AJ, Greenberg ME (1999) CREB: A stimulus-induced transcription factor activated by a diverse array of
extracellular signals. Annu Rev Biochem 68:821-861.
6. Silva AJ, Kogan JH, Frankland PW, Kida S (1998) CREB and memory. Annu Rev Neurosci 21:127-148.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CREB + ATF1 pSer133/Ser63 (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of control (1), EGF stimulated (2) or pervanadate treated (3) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab CREB/ATF1-10E9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 10E9 Purified Can, Hu, Ms E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CREB1 antibody

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining. Mouse IgG1 5G3 Ascites Hu, Mky, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CREB1 antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3F1-1B2 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CREB1 antibody

Immunoperoxidase of Monoclonal Antibody to CREB1 on formalin-fixed paraffin-embedded human kidney at 1 µg/ml. Mouse IgG2a 5F2 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CREB1 antibody

Immunoperoxidase of monoclonal antibody to CREB1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1 ug/ml] Mouse IgG2a 5F2 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CREB1 antibody

Western blot analysis of extracts from Jurkat cells, using CREB (Ab-100) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CREB1 antibody

Western blot analysis of CREB in 3T3 cell lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CREB1 (96-109) antibody

TA320216 (0.1ug/ml) staining of Jurkat (A) and NIH3T3 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CREB1 antibody

Predicted cell location: Nucleus. Positive control: Human cervical and breast cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using CREB1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CREB1 antibody

Predicted cell location: Nucleus. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using CREB1 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CREB1 (Center) antibody

CREB1 Antibody (Center) (Cat. #TA324428) western blot analysis in HT29 cell line lysates (35ug/lane).This demonstrates the CREB1 antibody detected the CREB1 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms F, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CREB1 antibody

Western blot analysis of CREB expression in PMA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CREB1 antibody

Western blot analysis of CREB expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CREB1 antibody

Western blot analysis on A549 and HuvEc cell lysate using CREB Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CREB1 antibody

Western blot analysis of extracts of various cell lines, using CREB1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CREB1 antibody

Western blot analysis of extracts from Hela cells untreated or treated with UV, using Phospho-CREB-Ser133 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

CREB1 antibody

Western Blot: CREB Antibody (82B514.2) [TA336386] - Analysis of CREB in HeLa cell lysate using this antibody at 2 ug/ml. Mouse IgG1 82B514.2 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CREB1 (N-term) antibody

Anti – CREB (DB 168)Western blot analysis of CREB. Lane A – 400ng of human recombinant CREB (1-342a.a., N-terminal GST tag; Novus Biologicals, cat. #: H00001385-P01). In 100ug of brain crude protein extract, Lane B – mouse; Lane C – rat. Rabbit IgG Q19-D Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 ml / €550.00
  DB Biotech, spol. s r.o.

Sample sizes available

CREB1 (N-term) antibody

Anti – CREB (DB 168)Western blot analysis of CREB. Lane A – 400ng of human recombinant CREB (1-342a.a., N-terminal GST tag; Novus Biologicals, cat. #: H00001385-P01). In 100ug of brain crude protein extract, Lane B – mouse; Lane C – rat. Rabbit IgG Q19-D Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
50 µl / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

CREB1 antibody

Profile of human IMR32 cell line analyzed by the BD FACSCanto II.Cells were fixed, permeabilized and stained with anti human CREB FITC (blue; used 10 μl per test) or with an isotype control (black). Rabbit IgG A17-A FITC Hu F
100 Tests / €570.00
  DB Biotech, spol. s r.o.

Sample sizes available

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