CSK antibody

Principal name

CSK antibody

Alternative names for CSK antibody

Tyrosine-protein kinase CSK, C-SRC kinase, Protein-tyrosine kinase CYL

SwissProt ID

P32577 (Rat), P41239 (Chick), P41240 (Human), P41241 (Mouse), Q0VBZ0 (Bovin)

Gene ID

1445 (CSK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Chk (Chicken), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of CSK antibody

All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine which is highly phosphorylated in the inactive form of the enzyme and phosphorylated to a much lesser extent when the enzyme is active. In the case of Src p60, Y527 is this tyrosine; however, a mutant form of c-Src in which Y527 is replaced by phenylalanine is transforming and displays 5- to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src-related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as carboxy terminal regulatory sequences. Csk phosphorylates Src on Y527 and also downregulates Lyn, Fyn and Lck by tyrosine phosphorylation of carboxy terminal regulatory sites.

General readings

1. Sabe H, Okada M, Nakagawa H, Hanafusa H: Activation of c-Src in cells bearing v-Crk and its suppression by Csk. Mol Cell Biol. 1992 Oct;12(10):4706-13.
2. Chow LM, Fournel M, Davidson D, Veillette A: Negative regulation of T-cell receptor signalling by tyrosine protein kinase p50csk. Nature. 1993 Sep 9;365(6442):156-60.
3. Bergman M, Joukov V, Virtanen I, Alitalo K: Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. Mol Cell Biol. 1995 Feb;15(2):711-22.
4. Takayama Y, Tanaka S, Nagai K, Okada M: Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999 Jan 22;274(4):2291-7.
5. Ingley E: Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta. 2008 Jan;1784(1):56-65.
6. Liang F, Luo Y, Dong Y, Walls CD, Liang J, Jiang HY, Sanford JR, Wek RC, Zhang ZY: Translational Control of C-terminal Src Kinase (Csk) Expression by PRL3 Phosphatase. J
Biol Chem. 2008 Apr 18;283(16):10339-46.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CSK antibody

Immunoperoxidase of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml] Mouse IgG1 3A3 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CSK (C-term) antibody

TA302657 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

CSK (C-term) antibody

TA302657 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CSK antibody

Western blot analysis of extracts from Jurkat cells, using CSK antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CSK antibody

WB Suggested Anti-CSK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CSK antibody

CSK Mouse IgG2b 2B4-C6-A10 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CSK antibody

Immunofluorescence analysis of U251 cells using CSK antibody Cat.-No AM06531SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 5F3 Ascites Hu, Mky, Ms, Rt E, F, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CSK antibody

CSK Rabbit Aff - Purified Hu WB
0.1 mg / €260.00
  Acris Antibodies GmbH

CSK antibody

Immunohistochemistry analyzes of Csk antibody (AP20298PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CSK antibody

Western blot (WB) analysis of Csk antibody (Cat.-No.: AP20417PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CSK antibody

Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Mouse IgG1 CSK-04 Purified Hu, Ms ICC/IF, IP, WB
0.1 ml / €260.00
  Acris Antibodies GmbH

CSK antibody

CSK Goat Purified Hu, Ms WB
0.1 mg / €550.00
  Acris Antibodies GmbH

CSK antibody

CSK Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CSK antibody

Western Blot analysis of CSK expression in transfected 293T cell line (H00001445-T03) by CSK MaxPab polyclonal antibody.

Lane 1: CSK transfected lysate(50.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CSK antibody

Immunofluorescence staining of CSK in human HeLa cell line using CSK monoclonal antibody, clone CSK - 04 ( Cat # MAB0950 ; green ) . Actin cytoskeleton was decorated by phalloidin ( red ) and cell nuclei stained with DAPI ( blue ) . Mouse IgG1 CSK-04 Hu, Ms ICC/IF, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

CSK antibody

CSK Mouse IgG2b PAG-C1 Bov, Hu, Ms, Rt IP
0.1 mg / €820.00
  Abnova Taiwan Corp.

CSK antibody

CSK polyclonal antibody ( Cat # PAB6039 ) staining ( 0.1 µg/ml ) of paraffin embedded human tonsil.  Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A ) Strong staining of germinal centres. B ) High magnification view of germinal centre cells. Goat Hu E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CSK pSer364 antibody

Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', using Csk (Phospho-Ser364) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CSK pSer364 antibody

Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', using Csk (Phospho-Ser364) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CSK antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CSK (Ab-364) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

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