CSK antibody

Principal name

CSK antibody

Alternative names for CSK antibody

Tyrosine-protein kinase CSK, C-SRC kinase, Protein-tyrosine kinase CYL

SwissProt ID

P32577 (Rat), P41239 (Chick), P41240 (Human), P41241 (Mouse), Q0VBZ0 (Bovin)

Gene ID

1445 (CSK), 315707 (Csk)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Mky (Monkey), Chk (Chicken), Ze (Zebrafish), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of CSK antibody

Kit Component:
- KN210758G1, CSK gRNA vector 1 in pCas-Guide vector
- KN210758G2, CSK gRNA vector 2 in pCas-Guide vector
- KN210758D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Sabe H, Okada M, Nakagawa H, Hanafusa H: Activation of c-Src in cells bearing v-Crk and its suppression by Csk. Mol Cell Biol. 1992 Oct;12(10):4706-13.
2. Chow LM, Fournel M, Davidson D, Veillette A: Negative regulation of T-cell receptor signalling by tyrosine protein kinase p50csk. Nature. 1993 Sep 9;365(6442):156-60.
3. Bergman M, Joukov V, Virtanen I, Alitalo K: Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. Mol Cell Biol. 1995 Feb;15(2):711-22.
4. Takayama Y, Tanaka S, Nagai K, Okada M: Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999 Jan 22;274(4):2291-7.
5. Ingley E: Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta. 2008 Jan;1784(1):56-65.
6. Liang F, Luo Y, Dong Y, Walls CD, Liang J, Jiang HY, Sanford JR, Wek RC, Zhang ZY: Translational Control of C-terminal Src Kinase (Csk) Expression by PRL3 Phosphatase. J
Biol Chem. 2008 Apr 18;283(16):10339-46.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CSK (C-term) antibody

  Goat Polyclonal Antibody against CSK  
TA302657 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CSK antibody

  Rabbit polyclonal anti-CSK antibody  
Western blot analysis of extracts from Jurkat cells, using CSK antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CSK antibody

  Rabbit Polyclonal Anti-CSK Antibody  
WB Suggested Anti-CSK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CSK antibody

  Mouse Monoclonal CSK Antibody  
CSK Mouse IgG2b 2B4-C6-A10 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CSK antibody

Immunofluorescence analysis of U251 cells using CSK antibody Cat.-No AM06531SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 5F3 Ascites Hu, Mky, Ms, Rt E, F, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CSK antibody

CSK Rabbit Aff - Purified Hu WB
0.1 mg / €260.00
  Acris Antibodies GmbH

CSK antibody

Immunohistochemistry analyzes of Csk antibody (AP20298PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CSK antibody

Western blot (WB) analysis of Csk antibody (Cat.-No.: AP20417PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CSK antibody

Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Mouse IgG1 CSK-04 Purified Hu, Ms ICC/IF, IP, WB
0.1 ml / €260.00
  Acris Antibodies GmbH

CSK antibody

CSK Goat Purified Hu, Ms WB
0.1 mg / €550.00
  Acris Antibodies GmbH

CSK pSer364 antibody

Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', using Csk (Phospho-Ser364) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

CSK pSer364 antibody

Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', using Csk (Phospho-Ser364) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CSK antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CSK (Ab-364) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

CSK antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CSK (Ab-364) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

CSK antibody

Immunofluorescence analysis of A549 cells, using CSK Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

CSK antibody

Immunofluorescence analysis of A549 cells, using CSK Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

CSK - human gene knockout kit via CRISPR

CSK - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CSK - human gene knockout kit via CRISPR

CSK - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CSK - human gene knockout kit via CRISPR

CSK - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CSK - human gene knockout kit via CRISPR

CSK - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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