CSTF1 antibody

Principal name

CSTF1 antibody

Alternative names for CSTF1 antibody

CF-1 50 kDa subunit, CstF-50, CstFp50, CSTF 50 kDa subunit, Cleavage stimulation factor 50 kDa subunit

SwissProt ID

Q05048 (Human), Q5BJQ6 (Rat), Q99LC2 (Mouse)

Gene ID

1477 (CSTF1), 67337 (Cstf1), 311670

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CSTF1 antibody

Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits of 77, 64 and 50 kDa. Heterotrimeric CstF recognizes GU and U-rich sequences located downstream of the polyadenylation site on RNA. The 50 kDa CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44 amino acid-long sequence that is repeated seven times. CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro. CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3'-end cleavage factors to form a stable complex on the pre-mRNA.

General readings

Takagaki. Y. and Manley J.L. (1992) J Biol Chem 267: 23471-23474Takagaki. Y. and Manley J.L. (2000) Mol Cell Biol 20: 1515-1525Kleiman. F.E. and Manley. J.L. (1999) Science 285: 1576-1579

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CSTF1 antibody

  Rabbit Polyclonal cleavage stimulation factor Antibody  
Immunohistochemical staining of human gall bladder shows strong nuclear staining with an additional slightly weaker cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CSTF1 antibody

  Rabbit polyclonal anti-CSTF1 antibody  
Western blot analysis of extracts from 293 cells and HUVEC cells, using CSTF1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CSTF1 antibody

The cell lysates of MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT10E11 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CSTF1 antibody

The cell lysates of MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT10E11 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

CSTF1 antibody

Western blot (WB) analysis of CstF-50 antibody (Cat.-No.: AP06715PU-N) in extracts from A549 cells. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CSTF1 antibody

Western blot analysis of extracts from HuvEc/293 cells, using CSTF1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

CSTF1 antibody

Western blot analysis of extracts from HuvEc/293 cells, using CSTF1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Cstf1 - mouse gene knockout kit via CRISPR

Cstf1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cstf1 - mouse gene knockout kit via CRISPR

Cstf1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cstf1 - mouse gene knockout kit via CRISPR

Cstf1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cstf1 - mouse gene knockout kit via CRISPR

Cstf1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CSTF1 (transcript variant 2)

  Recombinant protein of human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa (CSTF1), transcript variant 2  
CSTF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CSTF1 (transcript variant 3)

  Recombinant protein of human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa (CSTF1), transcript variant 3  
CSTF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CSTF1 (1-431, His-tag)

CSTF1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

CSTF1 (1-431, His-tag)

CSTF1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

CSTF1 overexpression lysate

CSTF1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

CSTF1 overexpression lysate

CSTF1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

CSTF1 overexpression lysate

CSTF1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

CSTF1 overexpression lysate

CSTF1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CSTF1 (mGFP-tagged) - Human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa (CSTF1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, CSTF1 (mGFP-tagged) - Human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa (CSTF1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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