CSTF1 antibody

Principal name

CSTF1 antibody

Alternative names for CSTF1 antibody

CF-1 50 kDa subunit, CstF-50, CstFp50, CSTF 50 kDa subunit, Cleavage stimulation factor 50 kDa subunit

SwissProt ID

Q05048 (Human), Q5BJQ6 (Rat), Q99LC2 (Mouse)

Gene ID

1477 (CSTF1), 3114771 (accA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CSTF1 antibody

Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits of 77, 64 and 50 kDa. Heterotrimeric CstF recognizes GU and U-rich sequences located downstream of the polyadenylation site on RNA. The 50 kDa CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44 amino acid-long sequence that is repeated seven times. CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro. CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3'-end cleavage factors to form a stable complex on the pre-mRNA.

General readings

Takagaki. Y. and Manley J.L. (1992) J Biol Chem 267: 23471-23474Takagaki. Y. and Manley J.L. (2000) Mol Cell Biol 20: 1515-1525Kleiman. F.E. and Manley. J.L. (1999) Science 285: 1576-1579

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CSTF1 antibody

Immunohistochemical staining of human gall bladder shows strong nuclear staining with an additional slightly weaker cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CSTF1 antibody

Western blot analysis of extracts from 293 cells and HUVEC cells, using CSTF1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CSTF1 antibody

The cell lysates of MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT10E11 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CSTF1 antibody

The cell lysates of MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT10E11 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

CSTF1 antibody

Western blot (WB) analysis of CstF-50 antibody (Cat.-No.: AP06715PU-N) in extracts from A549 cells. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CSTF1 antibody

CSTF1 Mouse IgG2a 1C6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CSTF1 antibody

Western blot analysis of extracts from HuvEc/293 cells, using CSTF1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CSTF1 antibody

Western blot analysis of extracts from HuvEc/293 cells, using CSTF1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CSTF1 antibody

Immunohistochemical analysis of CstF-50 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CSTF1 antibody

Immunohistochemical analysis of CstF-50 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CSTF1 antibody

Immunohistochemical analysis of CstF-50 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CSTF1 antibody

Western Blot: cleavage stimulation factor Antibody [27150002] - Samples:  Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11  Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil      Target weight [kDa]:  48       Validation score:  2   Validation description:  Supportive - Band of predicted size in kDa (+/-20%) with additional bands present. Rabbit Hu E, P, WB
50 µg / €440.00
  Novus Biologicals Inc.

CSTF1 antibody

Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - FFPE section of human ovarian carcinoma.  NB100-60443 used at a dilution of 1:250.  DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

CSTF1 antibody

Western Blot: CSTF50 Antibody [NB100-60444] - Detection of Human CSTF50 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CSTF50 antibody NB100-60444 used for IP at 3 ug/ml. For blotting immunoprecipitated CSTF50, affinity purified rabbit anti-CSTF50 antibody NB100-60443 was used at 1 ug/ml, and the ReliaBLOT Reagents and Procedures (WB120) were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CSTF1 (transcript variant 2)

CSTF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CSTF1 (transcript variant 3)

CSTF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CSTF1 (1-431, His-tag)

CSTF1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

CSTF1 (1-431, His-tag)

CSTF1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

CSTF1

CSTF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CSTF1

CSTF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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