CSTF2 antibody

Principal name

CSTF2 antibody

Alternative names for CSTF2 antibody

CSTF 64 kDa subunit, CstF-64, CSTF64, CF-1 64 kDa subunit, Cleavage stimulation factor 64 kDa subunit

SwissProt ID

P33240 (Human), Q8BIQ5 (Mouse), Q8HXM1 (Bovin)

Gene ID

1478 (CSTF2), 108062 (Cstf2)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of CSTF2 antibody

Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (also designated CSTF2) maps to the X chromosome, whereast CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CSTF2 antibody

Western blot analysis of extracts from 293 cells. HepG2 cells and Jurkat cells, using CSTF2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CSTF2 (betaCstF-64 variant 1/3) antibody

TA326335 (2ug) used to pull down ßCstf-64 from Mouse Brain (lane 5) lysate (500ug protein) while it cannot pull down Cstf-64 from Mouse Liver (lane 6)  lysate (500ug) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded Goat Aff - Purified Ms E, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

CSTF2 antibody

WB Suggested Anti-CSTF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate; CSTF2 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CSTF2 antibody

Western blot (WB) analysis of CstF-64 antibody (Cat.-No.: AP06681PU-N) in extracts from 293 cells. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CSTF2 (N-term) antibody

Western blot analysis of anti-CSTF2 (N-term) (AP11756PU-N) in NCI-H460 cell line lysates (35 µg/lane). CSTF2 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CSTF2 antibody

CSTF2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CSTF2 antibody

Western Blot analysis of CSTF2 expression in transfected 293T cell line (H00001478-T01) by CSTF2 MaxPab polyclonal antibody.

Lane 1: CSTF2 transfected lysate(63.47 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

CSTF2 antibody

CSTF2 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CSTF2 antibody

CSTF2 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

CSTF2 antibody

Western Blot analysis of CSTF2 expression in transfected 293T cell line (H00001478-T02) by CSTF2 MaxPab polyclonal antibody.

Lane 1: CSTF2 transfected lysate(61.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CSTF2 antibody

Immunoperoxidase of monoclonal antibody to CSTF2 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3ug/ml] Mouse IgG1 3D1-3A6 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CSTF2 antibody

Detection limit for recombinant GST tagged CSTF2 is approximately 1ng/ml as a capture antibody. Mouse IgG1 4E3-3C9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CSTF2 antibody

Western blot analysis of CSTF2 polyclonal antibody ( Cat # PAB3760 ) in Jurkat cell line lysate ( 35 µg/lane ) . CSTF2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CSTF2 antibody

Immunofluorescence analysis of HeLa cells, using CSTF2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

CSTF2 antibody

Immunofluorescence analysis of HeLa cells, using CSTF2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

CSTF2 antibody

Human 293T; WB Suggested Anti-CSTF2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. CSTF2 is supported by BioGPS gene expression data to be expressed in HEK293T.; CSTF2 antibody - N-terminal region (ARP40351_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CSTF2 antibody

Immunohistochemical analysis of CstF-64 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CSTF2 antibody

Immunohistochemical analysis of CstF-64 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CSTF2 antibody

Immunohistochemical analysis of CstF-64 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CSTF2 antibody

Western Blot and Immunoprecipitation: CSTF64 Antibody [NB100-57530] - Detection of Human CSTF64 on HeLa whole cell lysate using NB100-57530. CSTF64 was also immunoprecipitated by rabbit anti-CSTF64 antibody NB100-57531. Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

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