CSTF2 antibody

Principal name

CSTF2 antibody

Alternative names for CSTF2 antibody

CSTF 64 kDa subunit, CstF-64, CSTF64, CF-1 64 kDa subunit, Cleavage stimulation factor 64 kDa subunit

SwissProt ID

P33240 (Human), Q8BIQ5 (Mouse), Q8HXM1 (Bovin)

Gene ID

1478 (CSTF2), 108062 (Cstf2)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of CSTF2 antibody

Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (also designated CSTF2) maps to the X chromosome, whereast CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CSTF2 antibody

  Rabbit polyclonal anti-CSTF2 antibody  
Western blot analysis of extracts from 293 cells. HepG2 cells and Jurkat cells, using CSTF2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CSTF2 (betaCstF-64 variant 1/3) antibody

  Goat Anti-betaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse) Antibody  
TA326335 (2ug) used to pull down ßCstf-64 from Mouse Brain (lane 5) lysate (500ug protein) while it cannot pull down Cstf-64 from Mouse Liver (lane 6)  lysate (500ug) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded Goat Aff - Purified Ms E, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

CSTF2 antibody

  Rabbit Polyclonal Anti-CSTF2 Antibody  
WB Suggested Anti-CSTF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate; CSTF2 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CSTF2 antibody

Western blot (WB) analysis of CstF-64 antibody (Cat.-No.: AP06681PU-N) in extracts from 293 cells. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CSTF2 (N-term) antibody

Western blot analysis of anti-CSTF2 (N-term) (AP11756PU-N) in NCI-H460 cell line lysates (35 µg/lane). CSTF2 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CSTF2 antibody

Immunofluorescence analysis of HeLa cells, using CSTF2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

CSTF2 antibody

Immunofluorescence analysis of HeLa cells, using CSTF2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

CSTF2 - human gene knockout kit via CRISPR

CSTF2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CSTF2 - human gene knockout kit via CRISPR

CSTF2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CSTF2 - human gene knockout kit via CRISPR

CSTF2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CSTF2 - human gene knockout kit via CRISPR

CSTF2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cstf2 - mouse gene knockout kit via CRISPR

Cstf2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cstf2 - mouse gene knockout kit via CRISPR

Cstf2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cstf2 - mouse gene knockout kit via CRISPR

Cstf2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cstf2 - mouse gene knockout kit via CRISPR

Cstf2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CSTF2

  Recombinant protein of human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2)  
CSTF2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

CSTF2 overexpression lysate

CSTF2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CSTF2 (mGFP-tagged) - Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2), 200 uL, >10^7 TU/mL

Lenti ORF particles, CSTF2 (mGFP-tagged) - Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, CSTF2 (Myc-DDK tagged) - Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, CSTF2 (Myc-DDK tagged) - Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cstf2 (GFP-tagged) - Mouse cleavage stimulation factor, 3' pre-RNA subunit 2 (cDNA clone MGC:36412 IMAGE:5322335), 200 uL, >10^7 TU/mL

Lenti ORF particles, Cstf2 (GFP-tagged) - Mouse cleavage stimulation factor, 3' pre-RNA subunit 2 (cDNA clone MGC:36412 IMAGE:5322335), 200 uL, >10^7 TU/mL  
200 µl / €1,170.00
  OriGene Technologies, Inc.

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