CSTF3 antibody

Principal name

CSTF3 antibody

Alternative names for CSTF3 antibody

CSTF 77 kDa subunit, CstF-77, CSTF77, CF-1 77 kDa subunit, Cleavage stimulation factor 77 kDa subunit

SwissProt ID

Q12996 (Human), Q99LI7 (Mouse)

Gene ID

1479 (CSTF3)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of CSTF3 antibody

Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. CstF-77 (cleavage stimulation factor, 77 kDa subunit), also known as CstF3, is one of the three subunits comprising CstF. It can exist as a homodimer and functions as the bridge, directly interacting with the other two CstF subunits, namely CstF-64 and CstF-50. CstF-77 is highly conserved among eukaryotes. It contains an Alpha-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly. In addition, CstF-77 is capable of interacting with CPSF1 and FCP1, other factors involved in polyadenylation.

General readings

Benoit,B., (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (16), 10593-10598

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CSTF3 antibody

Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml] Mouse IgG1 1D4 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CSTF3 antibody

AP32732PU-N CSTF3 Antibody staining of nuclear HeLa lysate at  0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CSTF3 antibody

WB Suggested Anti-CSTF3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate; CSTF3 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CSTF3 antibody

WB Suggested Anti-CSTF3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CSTF3 antibody

CSTF3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CSTF3 antibody

Human 721_B; WB Suggested Anti-CSTF3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. CSTF3 is supported by BioGPS gene expression data to be expressed in 721_B.; CSTF3 antibody - middle region (ARP40353_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CSTF3 antibody

Human Jurkat; WB Suggested Anti-CSTF3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; CSTF3 antibody - N-terminal region (ARP40354_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CSTF3 antibody

Western Blot and Immunoprecipitation: CSTF77 Antibody [NB100-57532] - Detection of Human CSTF77 on HeLa whole cell lysate using NB100-57532. CSTF77 was also immunoprecipitated by rabbit anti-CSTF77 antibodies NB100-57533 and NB10-57534. Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CSTF3 antibody

Western Blot and IP: CSTF77 Antibody [NB100-57534] - Detection of Human and Mouse CSTF77 on HeLa whole cell lysate using NB100-57534. CSTF77 was also immunoprecipitated by rabbit anti-CSTF77 antibodies NB100-57532 and NB100-57533. Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CSTF3 antibody

Immunohistochemistry-Paraffin: CSTF3 Antibody [NBP1-85428] -  staining of human testis shows strong nuclear positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CSTF3 (transcript variant 1)

CSTF3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CSTF3

CSTF3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CSTF3

CSTF3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CSTF3 overexpression lysate

CSTF3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

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