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CTBP1 antibody

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Principal name

CTBP1 antibody

Alternative names for CTBP1 antibody

C-terminal-binding protein 1, CTBP

SwissProt ID

O88712 (Mouse), Q13363 (Human), Q9YHU0 (Xenla), Q9Z2F5 (Rat)

Gene ID

1487 (CTBP1), 13016 (Ctbp1), 29382

Ncbi ID

NP_001319, 9606, NP_001012632, NP_001012632.1, AAH53320, NM_001012614, NP_001185788.1, NM_013502, NM_019201

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Can (Canine), Eq (Equine), Por (Porcine), Bov (Bovine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of CTBP1 antibody

CTBP1, C-terminal-binding protein 1, is a protein that in humans is encoded by the CTBP1 gene. The CtBP1 protein binds to the C-terminus of adenovirus E1A proteins. This gene is mapped to 4p16.This phosphoprotein is a transcriptional repressor (corepressor) and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. CtBP1 and CtBP2 preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of δEF1, a cellular zinc finger-homeodomain protein, and thereby enhances δEF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

General readings

1. Lopez-Garcia J, Periyasamy M, Thomas RS, Christian M, Leao M, Jat P, Kindle KB, Heery DM, Parker MG, Buluwela L, Kamalati T, Ali S.: ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res. 2006; 34(21):6126-36.
2. Nadauld LD, Phelps R, Moore BC, Eisinger A, Sandoval IT, Chidester S, Peterson PW, Manos EJ, Sklow B, Burt RW, Jones D.A.: Adenomatous polyposis coli control of C-terminal
binding protein-1 stability regulates expression of intestinal retinol dehydrogenases. J Biol Chem. 2006 Dec 8;281(49):37828-35.
3. Valenta T, Lukas J, Korinek V.: HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res. 2003 May 1;31(9):2369-80.
4. Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V.: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun
7;25(11):2326-37.

ARP32468_P050 - CTBP1
Human Pancreas; CTBP1 antibody - C-terminal region (ARP32468_P050) in Human Pancreas cells using Immunohistochemistry
ARP32468_P050 - CTBP1
Human Jurkat; WB Suggested Anti-CTBP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. CTBP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; CTBP1 antibody - C-terminal region (ARP32468_P050) in Human Jurkat cells using Western Blot
H00001487-M01 - CTBP1
Immunoperoxidase of monoclonal antibody to CTBP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml]
H00001487-M01 - CTBP1
Immunofluorescence of monoclonal antibody to CTBP1 on HeLa cell. [antibody concentration 10 ug/ml]
H00001487-M01 - CTBP1
CTBP1 monoclonal antibody (M01), clone 2G7 Western Blot analysis of CTBP1 expression in HL-60 ( Cat # L014V1 ).
H00001487-M01 - CTBP1
Detection limit for recombinant GST tagged CTBP1 is approximately 1ng/ml as a capture antibody.
ARP32468_T100 - CTBP1
Human Jurkat; WB Suggested Anti-CTBP1 Antibody. . Titration: 1.25 ug/ml. . Positive Control: Jurkat Whole Cell. CTBP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; CTBP1 antibody - C-terminal region (ARP32468_T100) in Human Jurkat cells using Western Blot
AR09394PU-L - CTBP1
AR09394PU-L - CTBP1
Recombinant human CtBP1, 1-440 aa: 15% SDS-PAGE (3 µg)
A8325-1 - CTBP1
Immunohistochemistry analysis of paraffin-embedded human brain, using CtBP1 (Phospho-Ser422) Antibody. The picture on the right is treated with the synthesized peptide.
A8325-1 - CTBP1
Western blot analysis of extracts from Jurkat cells treated with TNF 20ng/ml 30' and Jurkat cells treated with UV 15', using CtBP1 (Phospho-Ser422) Antibody. The lane on the right is treated with the synthesized peptide.
B8325-1 - CTBP1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CtBP1 (Ab-422) Antibody. The picture on the right is treated with the synthesized peptide.
B8325-1 - CTBP1
Western blot analysis of extracts from Jurkat cells, using CtBP1 (Ab-422) Antibody. The lane on the right is treated with the synthesized peptide.
AM09387PU-N - CTBP1
Immunofluorescence: ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
AM09387PU-N - CTBP1
Western Blot: The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa cell lysate. Lane 2: NIH3T3 cell lysate. Lane 3: 293T cell lysate. Lane 4: AGS cell lysate. Lane 5: Raji cell lysate. Lane 6: A549 cell lysate. Lane 7: Jurkat cell lysate.
AP51115PU-N - CTBP1
CTBP1 Antibody (C-term) (Cat. #AP51115PU-N) western blot analysis in mouse Neuro-2a cell line lysates (35µg/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow).
AP32227PU-N - CTBP1
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP32227PU-N - CTBP1
Western blot analysis of extracts from Jurkat cells, using CtBP1 Antibody Cat.-No AP32227PU-N. The lane on the right is treated with the synthesized peptide.
AP55814PU-S - CTBP1
Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody AP55814PU-N (left)or the same antibody preincubated with blocking peptide (right).
AP55814PU-S - CTBP1
Western blot analysis of extracts from Jurkat cells treated with TNF using CtBP1 (Phospho-Ser422) Antibody AP55814PU-N.The lane on the right is treated with the antigen-specific peptide.
TP308594 - CTBP1
TP310529 - CTBP1
TA308690 - CTBP1
Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. TA308690 diluted at 1:1000.
TA308690 - CTBP1
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CtBP1(TA308690) antibody at 1:200 dilution.
TA313245 - CTBP1
Western blot analysis of extracts from Jurkat cells, using CtBP1 (Ab-422) antibody.The lane on the right is treated with the synthesized peptide.
TA313245 - CTBP1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CtBP1 (Ab-422) antibody.The picture on the right is treated with the synthesized peptide.
TA324588 - CTBP1
CTBP1 Antibody (C-term) (Cat. #TA324588) western blot analysis in Hela,HL-60,Jurkat,Raji cell line and mouse brain,rat brain tissue lysates (35ug/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow).
TA324588 - CTBP1
IF image of Hela cell stained with CTBP1 Antibody (C-term)(Cat#TA324588). Hela cells were incubated with CTBP1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .CTBP1 immunoreactivity is localized to nucleus significantly.
TA324588 - CTBP1
IF image of SK-BR-3 cell stained with CTBP1 Antibody (C-term)(Cat#TA324588). SK-BR-3 cells were incubated with CTBP1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .CTBP1 immunoreactivity is localized to nucleus significantly.
TA327149 - CTBP1
Western blot analysis of extracts of various cell lines, using CTBP1 antibody.
TA327149 - CTBP1
Immunohistochemistry of paraffin-embedded human stomach cancer using CTBP1 antibody at dilution of 1:200 (200x lens).
TA327149 - CTBP1
Immunofluorescence analysis of U2OS cell using CTBP1 antibody. Blue: DAPI for nuclear staining.
TA330555 - CTBP1
WB Suggested Anti-CTBP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateCTBP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
TA330555 - CTBP1
Human Pancreas
TA330555 - CTBP1
Human Pineal Tissue
TA330556 - CTBP1
WB Suggested Anti-CTBP1 Antibody; Titration: 1.25 ug/ml; Positive Control: Jurkat Whole CellCTBP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
TA330557 - CTBP1
WB Suggested Anti-CTBP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver
TA347791 - CTBP1
Western blot analysis on Jurkat cell lysate using CtBP1 Antibody
TA351112 - CTBP1
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (CTBP1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
TA351112 - CTBP1
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (CTBP1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA351112 - CTBP1
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CTBP1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA348444 - CTBP1
Western blot analysis on Jurkat cell lysate using Phospho-CtBP1(Ser422) Antibody
TA313246 - CTBP1
Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using CtBP1 (Phospho-Ser422) antibody.The lane on the right is treated with the synthesized peptide.
TA313246 - CTBP1
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody.The picture on the right is treated with the synthesized peptide.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CTBP1 antibody
  Rabbit polyclonal antibody to CtBP1 (C-terminal binding protein 1)  
Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. TA308690 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

CTBP1 antibody
  Rabbit polyclonal CtBP1 (Ab-422) antibody  
Western blot analysis of extracts from Jurkat cells, using CtBP1 (Ab-422) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

CTBP1 antibody
  Rabbit polyclonal CtBP1 (Ser422) antibody(Phospho-specific)  
Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using CtBP1 (Phospho-Ser422) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

CTBP1 (C-term) antibody
  Rabbit polyclonal CTBP1 Antibody (C-term)  
CTBP1 Antibody (C-term) (Cat. #TA324588) western blot analysis in Hela,HL-60,Jurkat,Raji cell line and mouse brain,rat brain tissue lysates (35ug/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.
+2 additional images

CTBP1 antibody
  Rabbit anti-CTBP1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CTBP1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.
+2 additional images

CTBP1 antibody
  Rabbit Polyclonal Anti-CTBP1 Antibody  
WB Suggested Anti-CTBP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateCTBP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.
+2 additional images

CTBP1 antibody
  Rabbit Polyclonal Anti-CTBP1 Antibody  
WB Suggested Anti-CTBP1 Antibody; Titration: 1.25 ug/ml; Positive Control: Jurkat Whole CellCTBP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CTBP1 antibody
  Rabbit Polyclonal Anti-CTBP1 Antibody  
WB Suggested Anti-CTBP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CTBP1 antibody
  Rabbit Polyclonal Anti-CtBP1 Antibody  
Western blot analysis on Jurkat cell lysate using CtBP1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CTBP1 antibody
  Rabbit Polyclonal Anti-Phospho-CtBP1(Ser422) Antibody  
Western blot analysis on Jurkat cell lysate using Phospho-CtBP1(Ser422) Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CTBP1 antibody
  Rabbit Polyclonal Anti-CTBP1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (CTBP1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+2 additional images

CTBP1 antibody
  Rabbit Polyclonal Anti-CTBP1 Antibody  
CTBP1 Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CTBP1 antibody
Immunofluorescence: ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Mouse IgG2b AT4D6 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH
+1 additional image

CTBP1 antibody
Immunofluorescence: ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Mouse IgG2b AT4D6 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH
+1 additional image

CTBP1 antibody
CTBP1 Rabbit Aff - Purified Hu, Ms ICC/IF, IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

CTBP1 antibody
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH
+1 additional image

CTBP1 (C-term) antibody
CTBP1 Antibody (C-term) (Cat. #AP51115PU-N) western blot analysis in mouse Neuro-2a cell line lysates (35µg/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CTBP1 pSer422 antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody AP55814PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €340.00
  Acris Antibodies GmbH
+1 additional image

CTBP1 pSer422 antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody AP55814PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €270.00
  Acris Antibodies GmbH
+1 additional image

CTBP1 pSer422 antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using CtBP1 (Phospho-Ser422) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech
+1 additional image

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