CTCF antibody

Principal name

CTCF antibody

Alternative names for CTCF antibody

CCCTC-binding factor, CTCFL paralog, Transcriptional repressor CTCF, 11-zinc finger protein

SwissProt ID

P49711 (Human), Q08705 (Chick), Q61164 (Mouse), Q9R1D1 (Rat)

Gene ID

10664 (CTCF), 83726 (Ctcf)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of CTCF antibody

CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms\' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms\' tumors.

General readings

Defossez,P.A., (2005) J. Biol. Chem. 280 (52), 43017-43023

16 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CTCF antibody

Western blot analysis of Boris expression in lysate from Jurkat cells (Lane 1 & 2), 3T3 cells (Lane 3) and Rat kidney (Lane 4). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CTCF antibody

Western Blot of Rabbit Anti-CTCF antibody. Lane 1: H929 nuclear cell lysates. Load: 30 µg per lane. Primary antibody: CTCF antibody at 1.2 µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 3% BSA overnight at 4°C. Predicted/Observed size: 82.8 kDa or 150 kDa for CTCF. Other band(s): CTCF splice variants. Rabbit IgG Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CTCF antibody

WB Suggested Anti-CTCF Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Hu ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CTCF antibody

WB Suggested Anti-CTCF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CTCF antibody

WB using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

CTCF antibody

Western blot analysis using CTCF antibody Cat.-No AM06561SU-N against A31 (1), MCF-7 (2), Hela (3), HCT116 (4), Jurkat (5), NIH/3T3 (6), and Cos7 (7) cell lysate. Mouse IgG1 1D11 Ascites Hu, Mky E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CTCF antibody

CTCF polyclonal antibody (A01), Lot # 050727JC01 Western Blot analysis of CTCF expression in HL-60 ( Cat # L014V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CTCF antibody

Western blot analysis of Ctcf expression in lysate from Jurkat cells ( Lane 1 and 2 ) , NIH/3T3 cells ( Lane 3 ) and rat kidney ( Lane 4 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CTCF antibody

Transfected 293T; WB Suggested Anti-CTCF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; CTCF antibody - N-terminal region (ARP38820_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CTCF antibody

Human 293T; WB Suggested Anti-CTCF Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; CTCF antibody - N-terminal region (P100757_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CTCF antibody

Immunohistochemistry: CTCF Antibody [NB100-2161] - Detection of Human CTCF by Immunohistochemistry.  Samples:  FFPE section of human ovarian  carcinoma.  Antibody:  Affinity purified rabbit anti-CTCF (NB100-2161) used at a dilution of  1:250.  Detection: DAB Rabbit Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

CTCF antibody

Immunoprecipitation: CTCF Antibody [NB500-175] - Immunoprecipitation of Human CTCF.  Samples: Whole cell  lysate (1 mg for IP; 1/4 of IP loaded/lane) from MCF7 cells.  Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-175   used at 3 mcg/mg lysate for IP. Human  CTCF was also immunoprecipitated using affinity purified rabbit  anti-CTCF antibodies NB500-177 and  NB500-194 CTCF was blotted using  NB500-177 at 0.03 mcg/ml. Detection: Chemiluminescence with  exposure times of 10 seconds. Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

CTCF (C-term) antibody

Immunoprecipitation: CTCF Antibody [NB500-194] - Immunoprecipitation of Human CTCF.  Samples: Whole cell  lysate (1 mg for IP; 1/4 of IP loaded/lane) from MCF7 cells.  Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-194   used at 3 mcg/mg lysate for IP. Human  CTCF was also immunoprecipitated using affinity purified rabbit  anti-CTCF antibodies NB500-175 and  NB500-177 CTCF was blotted using  NB500-177 at 0.03 mcg/ml. Detection: Chemiluminescence with  exposure times of 10 seconds. Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CTCF

CTCF Human
2 µg / €300.00
  Abnova Taiwan Corp.

CTCF

CTCF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CTCF

CTCF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

16 Item(s)

per page
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