CTCFL / BORIS antibody

Principal name

CTCFL / BORIS antibody

Alternative names for CTCFL / BORIS antibody

CTCF-like protein, CTCF paralog, CCCTC-binding factor, CTCF-T, CT27, Transcriptional repressor CTCFL, Brother of the regulator of imprinted sites, Zinc finger protein CTCF-T, Cancer/testis antigen 27

SwissProt ID

Q8NI51 (Human)

Gene ID

140690 (CTCFL)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of CTCFL / BORIS antibody

Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.

General readings

Klenova EM et al. The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer. Semin Cancer Biol 12:399-414 (2002). PubMed PMID: 12191639
Loukinov DI et al. BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma. Proc Natl Acad Sci U S A 99:6806-11 (2002). PubMed PMID: 12011441

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CTCFL / BORIS (9-26) antibody

Western blot using Affinity Purified anti-BORIS antibody shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead).   Lane 1 contains lysate from human prostate tissue.  Lane 2 contains lysate from human spleen tissue.  A predominant band at ~75 kDa is observed.  Molecular weight estimation was made by comparison to prestained MW markers as indicated. Rabbit IgG Aff - Purified Chimp, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CTCFL / BORIS antibody

WB Suggested Anti-CTCFL Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysateCTCFL is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CTCFL / BORIS antibody

WB Suggested Anti-CTCFL Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysateCTCFL is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CTCFL / BORIS antibody

Western blot using CTCFL polyclonal antibody ( Cat # PAB9983 ) shows detection of a predominant band corresponding to BORIS in human tissue lysates ( arrowhead ).
Lane 1 contains lysate from human prostate tissue.
Lane 2 contains lysate from human spleen tissue.
Apredominant band at ~75 kDa is observed.
Molecular weight estimation was made by comparison to prestained MW markers as indicated. Chimp, Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

CTCFL / BORIS antibody

Human Jurkat; WB Suggested Anti-CTCFL Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. CTCFL is supported by BioGPS gene expression data to be expressed in Jurkat.; CTCFL antibody - N-terminal region (ARP36127_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CTCFL / BORIS antibody

Human 721_B; WB Suggested Anti-CTCFL Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. CTCFL is supported by BioGPS gene expression data to be expressed in 721_B.; CTCFL antibody - N-terminal region (ARP36128_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CTCFL / BORIS antibody

Western Blot: BORIS Antibody [NB100-2028] - detection of a predominant  band corresponding to BORIS in  human tissue lysates (arrowhead).  Lane 1 contains lysate from human  prostate tissue. Lane 2 contains lysate  from human spleen tissue. A  predominant band at ~75 kDa is  observed. Molecular weight estimation  was made by comparison to  prestained MW markers as indicated Rabbit Aff - Purified Hu E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

CTCFL / BORIS antibody

Western Blot: BORIS Antibody [NBP1-45409] - Western blot analysis of
Boris expression in lysate
from Jurkat cells (Lane 1 &
2), 3T3 cells (Lane 3) and
Rat kidney (Lane 4). Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €440.00
  Novus Biologicals Inc.

CTCFL / BORIS antibody

Immunohistochemistry-Paraffin: BORIS Antibody [NBP1-89947] - Immunohistochemical staining of human testis shows strong nuclear positivity in primary spermatocytes. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CTCFL / BORIS

CTCFL / BORIS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CTCFL / BORIS

CTCFL / BORIS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CTCFL overexpression lysate

CTCFL overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

12 Item(s)

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