CTGF antibody

Principal name

CTGF antibody

Alternative names for CTGF antibody

CCN2, HCS24, IGFBP8, Connective tissue growth factor, IGF-binding protein 8,IBP8, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, CCN family member 2

SwissProt ID

O18739 (Bovin), O19113 (Pig), P29268 (Mouse), P29279 (Human), Q9R1E9 (Rat)

Gene ID

1490 (CTGF), 1490785 (gnd), 14219

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Chk (Chicken), Ms (Mouse), Por (Porcine), Sh (Sheep), Eq (Equine), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Functional assay (FN), Immunoprecipitation (IP)

Background of CTGF antibody

Connective Tissue Growth Factor belongs to the CCN family of proteins. The CCN family presently consists of six members in human also known as: Cyr61 (Cystein rich 61), CTGF (Connective Tissue Growth Factor), Nov (Nephroblastoma Overexpressed gene), WISP-1, 2 and 3 (Wnt-1 Induced Secreted Proteins). The CCN genes encode secreted proteins associated with the Extracellular Matrix (ECM) and cell membrane. CCN proteins are matricellular proteins which are involved in the regulation of various cellular functions including: proliferation, differentiation, survival, adhesion and migration. They are expressed in derivatives of the three embryonic sheets and are implicated in the development of kidney, nervous system, muscle, bone marrow, cartilage and bone. During adulthood, they are implicated in wound healing, bone fracture repair, and pathologies such as: fibrosis, vascular ailments and tumourigenesis. Full length secreted CCN proteins can show an antiproliferative activity, whereas truncated isoforms are likely to stimulate proliferation and behave as oncogenes. The full-length protein consists of four modules:
Module I shares partial identity with the N-terminal part of the Insulin-like Growth Factor Binding Proteins (IGFBPs).
Module II includes a stretch of 70amino acid residues - which shares sequence identity with the Von Willebrand Factor Type C repeat (VWC).
Module III contains sequences sharing identity with the Thrombospondin type 1 repeat (TSP1) (WSXCSXXCG), which is thought to be implicated in the binding of sulfated glycoconjugates and to be important for cell adhesion.
Module IV, also designated CT, is encoded by exon 5. It is the least conserved one of the four domains at the level of nucleotide sequence, but it appears to be critical for several of the biological functions attributed to the CCN proteins. Module IV resembles the CT domain of several extracellular protein including, Von Willebrand's factor and mucins. Sequence similarities to heparin-binding motifs are also found within this domain.

Proteolysis of the secreted full-length CCN proteins that has been reported in the case of CCN2 and CCN3 might result in the production of CCN-derived peptides with high affinity for ligands that full-length CNN proteins bind only poorly. Amino-truncated CCN2 isoforms were biologically active whereas no specific biological activity has been attributed to the truncated CCN3. Although the molecular processes underlying the production of these secreted isoforms is presently unknown, it is important to note that proteolysis occur at the same amino acid residues in both CCN2 and CCN3. An elevated expression of CCN2 has also been detected by Northern blotting in human invasive mammary ductal carcinomas, dermatofibromas, pyogenic granuloma, endothelial cells of angiolipomas and angioleiomyomas, and in pancreatic tumours. A study performed with chondrosarcomas representative of various histological grades established that CCN2 expression was closely correlated with increasing levels of malignancy.In agreement with CCN2 playing a role in brain tumour angiogenesis, immunocytochemistry studies indicated that both glioblastoma tumour cells and proliferating endothelial cells stained positive for CCN2. In astrocytomas, CCN2 expression was particularly elevated in high-grade tumours, with a marked effect of CCN2 on cell proliferation. Downregulation of CCN2 expression in these cells was associated with a growth arrest at the G1/S transition while over-expression of CCN2 induced a two-fold increase of the number of cells in the G1 phase. Gene profiling analysis allowed to identify a set of about 50 genes whose expression might account for the proliferative activity of CCN2 in these cells. CCN2 was seen in a higher proportion of mononuclear cells of patients with acute lymphoblastic leukemia.

General readings

Jun JI., et al. (2011) Nat Rev Drug Discov, 10 (12): 945–63

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CTGF antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CTGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806797) Mouse IgG1 OTI2C9 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CTGF antibody

Detection of CTGF in mouse kidney lysate. 5 sec. ECL exposure. Rabbit IgG Purified Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CTGF antibody

Predicted band size: 38kDa. Positive control: Hela cell lysate . Recommended dilution: 1/500-2000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CTGF antibody

To detect hCTGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CTGF antibody

To detect hCTGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGF (TA328283) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CTGF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTGF (RC210336, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTGF. Mouse IgG2a OTI5C10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CTGF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTGF (RC210336, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTGF. Mouse IgG2a OTI2G3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CTGF antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CTGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806802) Mouse IgG2b OTI6D1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CTGF antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTGF mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA806803)(1:150) Mouse IgG2a OTI5H7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CTGF antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CTGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806838) Mouse IgG2a OTI4B12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CTGF antibody

CTGF Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

CTGF antibody

CTGF Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

CTGF antibody

Sandwich ELISA using AP01150PU-N CTGF Antibody (Cat.-No AP01150PU) Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CTGF antibody

Sandwich ELISA using AP01150PU-N CTGF Antibody (Cat.-No AP01150PU) Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

CTGF (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CTGF antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

CTGF (C-term) antibody

CTGF Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CTGF antibody

BM4099 CTGF antibody staining of Frozen Human umbilical cord Section (Clone 2154-60) Mouse IgM 2154-60 Purified Hu, Rt C, P
0.5 mg / €570.00
  Acris Antibodies GmbH

CTGF antibody

CTGF monoclonal antibody (M01), clone 2C11. Western Blot analysis of CTGF expression in human kidney. Mouse IgG2b 2C11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CTGF antibody

CTGF Mouse IgG2b 4D9 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

CTGF antibody

Western blot analysis of CTGF in mouse kidney lysate using CTGF polyclonal antibody ( Cat # PAB12524 ). Rabbit Bov, Chk, Hu, Ms, Rt, Sh WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

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