Cullin 1 antibody

Principal name

Cullin 1 antibody

Alternative names for Cullin 1 antibody

Cullin-1, CUL-1, CUL1

SwissProt ID

O13790 (Schpo), Q13616 (Human), Q17389 (Caeel), Q24311 (Drome), Q94AH6 (Arath), Q9WTX6 (Mouse)

Gene ID

8454 (CUL1), 26965 (Cul1)

Available reactivities

Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of Cullin 1 antibody

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1L, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110 (By similarity).

General readings

Specific Reference(s):
1. Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449.
2. Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839.
3. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541.
4. Mathias, N., Johnson, S.L., Winey, M., Adams, A.E., Goetsch, L., Pringle, J.R., Byers, B., Goebl, M.G. (1996) Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins. Mol Cell Biol 16:12, 6634-6643.
General Reference(s):
1. Hori,T., Osaka,F., Chiba,T., Miyamoto,C., Okabayashi,K., Shimbara,N., Kato,S. and Tanaka,K. (1999) Covalent modification of all members of human cullin family proteins by NEDD8. Oncogene 18 (48), 6829-6834.
2. Read,M.A., Brownell,J.E., Gladysheva,T.B., Hottelet,M., Parent,L.A., Coggins,M.B., Pierce,J.W., Podust,V.N., Luo,R.-S., Chau,V. and Palombella,V.J. (2000) Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha. Mol. Cell. Biol. 20 (7), 2326-2333.
3. Tan,P., Fuchs,S.Y., Chen,A., Wu,K., Gomez,C., Ronai,Z. and Pan,Z.Q. (1999) Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha. Mol. Cell 3 (4), 527-533 (1999).
4. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties. Trends Cell Biol. 10(8):335-42.

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Catalog No. Species Donor Vector  

CUL1 - human gene knockout kit via CRISPR

CUL1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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