Cullin-3 antibody

Principal name

Cullin-3 antibody

Alternative names for Cullin-3 antibody

CUL-3, CUL3, KIAA0617

SwissProt ID

Q13618 (Human), Q9JLV5 (Mouse)

Gene ID

8452 (CUL3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of Cullin-3 antibody

Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cullin-3 antibody

Cullin 3 antibody detects Cullin 3 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human CUL3-transfected 293T cells. 7.5 % SDS-PAGE. Cullin 3 antibody (TA308790) dilution: 1:5000 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using Cullin 3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cullin-3 (N-term) antibody

Anti-CUL3 antibody was diluted 1:500 to detect CUL3 in human breast tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Rabbit Serum Hu, Ms E, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

Western blot analysis of Jurkat cell lysate using CUL3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

TA329091 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml; Positive Control: Fetal liver cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Hu, Ms E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Cullin-3 antibody

Cullin-3 Mouse IgG1 1A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cullin-3 antibody

Immunohistochemical staining with CUL3 polyclonal antibody ( Cat # PAB10037 ) was diluted 1 : 500 to detect CUL3 in human breast tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Hu, Ms
0.1 ml / €560.00
  Abnova Taiwan Corp.

Cullin-3 antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using Cullin 3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

Cullin-3 antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using Cullin 3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

Cullin-3 (C-term) antibody

Human Testis; Immunohistochemistry with Testis tissue at an antibody concentration of 10ug/ml using anti-CUL3 antibody (ARP61264_P050); CUL3 antibody - C-terminal region (ARP61264_P050) in Human Testis cells using Immunohistochemistry Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Cullin-3 antibody

Cullin-3 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

Cullin-3 antibody

Immunohistochemical analysis of Cullin 3 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Cullin-3 antibody

Immunohistochemical analysis of Cullin 3 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Cullin-3 antibody

Immunohistochemical analysis of Cullin 3 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Cullin-3 antibody

Western Blot: Cul3 Antibody [NB100-58787] - Detection of Human Cul3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-Cul3 antibody NB100-58787 used for IP at 3 mcg/mg lysate. Cul3 was also immunoprecipitated by other rabbit anti-Cul3 antibodies NB100-58788 and NB100-58789, which recognize downstream epitopes.  Detection:  Chemiluminescence with an exposure time of 10 seconds. Rabbit Aff - Purified Hu ICC/IF, IP, P
0.1 mg / €440.00
  Novus Biologicals Inc.

Cullin-3 antibody

Western Blot: Cul3 Antibody [NB100-58788] -  Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes). Rabbit Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cullin-3

Cullin-3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Cullin-3

Cullin-3 Human
2 µg / €300.00
  Abnova Taiwan Corp.

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