Cullin-3 antibody

Principal name

Cullin-3 antibody

Alternative names for Cullin-3 antibody

CUL-3, CUL3, KIAA0617

SwissProt ID

Q13618 (Human), Q9JLV5 (Mouse)

Gene ID

8452 (CUL3), 26554 (Cul3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of Cullin-3 antibody

Cullin-3(Cul 3), is a protein that in humans is encoded by the CUL3 gene. Kipreos et al. (1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Human CUL3 is an ortholog of nematode cul3. Hartz (2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37). Sumara et al. (2007) found that KLHL9, KLHL13, and CUL3 interacted directly in a 370-kD protein complex in HeLa cell lysates. Rondou et al. (2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4 (DRD4). KLHL12 interacted directly with CUL3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al. (2011) showed that KLHL7 interacted with CUL3 and ROC1 (RBX1).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cullin-3 antibody

  Rabbit polyclonal antibody to CUL-3 (cullin 3)  
Cullin 3 antibody detects Cullin 3 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human CUL3-transfected 293T cells. 7.5 % SDS-PAGE. Cullin 3 antibody (TA308790) dilution: 1:5000 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

  Rabbit polyclonal anti-Cullin 3 antibody  
Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using Cullin 3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cullin-3 (N-term) antibody

  Rabbit polyclonal anti-Cul3 antibody  
Anti-CUL3 antibody was diluted 1:500 to detect CUL3 in human breast tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Rabbit Serum Hu, Ms E, IP, P, WB
0.1 ml / €356.00
  OriGene Technologies, Inc.

Cullin-3 antibody

  Rabbit anti-CUL3 Polyclonal Antibody  
Western blot analysis of extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins), using ITGB4 (Phospho-Tyr1510) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

  Cullin 3 Goat Polyclonal Antibody  
TA329091 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

  Rabbit Polyclonal Anti-CUL3 Antibody  
WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml; Positive Control: Fetal liver cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

  Rabbit Polyclonal Anti-CUL3 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (CUL3 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cullin-3 antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Cullin-3 antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using Cullin 3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €230.00
  Assay Biotech

Cullin-3 antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using Cullin 3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

CUL3 - human gene knockout kit via CRISPR

CUL3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CUL3 - human gene knockout kit via CRISPR

CUL3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CUL3 - human gene knockout kit via CRISPR

CUL3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CUL3 - human gene knockout kit via CRISPR

CUL3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cul3 - mouse gene knockout kit via CRISPR

Cul3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cul3 - mouse gene knockout kit via CRISPR

Cul3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cul3 - mouse gene knockout kit via CRISPR

Cul3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cul3 - mouse gene knockout kit via CRISPR

Cul3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cullin-3

  Recombinant protein of human cullin 3 (CUL3)  
Cullin-3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cul3 (GFP-tagged) - Mouse cullin 3 (Cul3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Cul3 (GFP-tagged) - Mouse cullin 3 (Cul3), 200 uL, >10^7 TU/mL  
200 µl / €1,470.00
  OriGene Technologies, Inc.

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