Cullin-4B antibody
Principal name
Cullin-4B antibody
Alternative names for Cullin-4B antibody
CUL-4B, CUL4B, KIAA0695
SwissProt ID
Q13620 (Human)
Gene ID
8450 (CUL4B)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of Cullin-4B antibody
Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING (really interesting new gene) ubiquitin ligase (E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain (cullin homology domain) and a series of N-terminal repeats (cullin repeats). Ohtake et al. (2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor (AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B(AhR). Complex assembly and ubiquitin ligase activity of CUL4B(AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B(AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.
Immunofluorescence of purified MaxPab antibody to CUL4B on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CUL4B expression in transfected 293T cell line (H00008450-T01) by CUL4B MaxPab polyclonal antibody.Lane 1: CUL4B transfected lysate(100.43 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of CUL4B on formalin fixed, paraffin embedded Zebrafish body with CUL4B polyclonal antibody ( Cat # PAB12320 ).
Western Blot analysis of CUL4B expression from cell and tissue extracts with CUL4B polyclonal antibody ( Cat # PAB12320 ).Lane 1 : female zebrafish tissue lysate.Lane 2 : MM453 whole cell lysate.Lane 3 : rat liver tissue lysate.Lane 4 : rat lung tissue lysate.Lane 5 : MCF-7 whole cell lysate.Lane 6 : MM231 whole cell lysate.
Cullin 4B CUL4B Polyclonal Antibody
Cullin 4B CUL4B Polyclonal Antibody
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CUL4B Rabbit Polyclonal antibody. (TA590752)
Immunohistochemical staining of human esophagus shows nuclear positivity in squamous epithelial cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUL4B (RC220405, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUL4B.
ELISA: CUL4B Antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUL4B (RC206935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUL4B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUL4B (RC206935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUL4B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CUL4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CUL4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800444)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CUL4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800444)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CUL4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800444)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CUL4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800444)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUL4B (RC206935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUL4B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CUL4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of CUL4B Antibody (Center) Pab (Cat. #TA324698) pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. CUL4B Antibody (Center) (arrow) was detected using the purified Pab.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CUL4B Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using (CUL4B Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: HepG2 cells, Primary antibody: (CUL4B Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA590752 | Cullin-4B antibody |
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| Rabbit Polyclonal CUL4B Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€409.00
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| OriGene Technologies, Inc. | |||||||
 +16 additional images |
Highly validated antibody! |
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| TA800444 | Cullin-4B antibody |
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| CUL4B mouse monoclonal antibody, clone OTI1C4 (formerly 1C4) | |||||||
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Mouse | IgG2b | OTI1C4 | Purified | Can, Hu, Ms | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA324698 | Cullin-4B antibody |
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| Rabbit polyclonal CUL4B Antibody (Center) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.4 ml /
€410.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351114 | Cullin-4B antibody |
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| Rabbit Polyclonal Anti-CUL4B Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351115 | Cullin-4B antibody |
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| Rabbit Polyclonal Anti-CUL4B Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA800391 | Cullin-4B antibody |
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| CUL4B mouse monoclonal antibody, clone OTI7G10 (formerly 7G10) | |||||||
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Mouse | IgG1 | OTI7G10 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA800411 | Cullin-4B antibody |
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| CUL4B mouse monoclonal antibody, clone OTI7F8 (formerly 7F8) | |||||||
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Mouse | IgG1 | OTI7F8 | Purified | Can, Hu, Mky, Ms, Rt | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP23317PU-N | Cullin-4B antibody |
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Rabbit | IgG | Aff - Purified | Hu, Rt, Ze | P, WB |
0.1 mg /
€390.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN206935 | CUL4B - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206935BN | CUL4B - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206935LP | CUL4B - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206935RB | CUL4B - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306935 | Cullin-4B (transcript variant 1) |
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| Recombinant protein of human cullin 4B (CUL4B), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
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| OriGene Technologies, Inc. | |||||
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