Cullin-5 antibody

Principal name

Cullin-5 antibody

Alternative names for Cullin-5 antibody

CUL-5, CUL5, VACM1, VACM-1, Vasopressin-activated calcium-mobilizing receptor

SwissProt ID

Q29425 (Rabit), Q93034 (Human), Q9D5V5 (Mouse), Q9JJ31 (Rat)

Gene ID

8065 (CUL5), 75717 (Cul5), 64624

Available reactivities

Hu (Human), Can (Canine), Dros (Drosophila), Ms (Mouse), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of Cullin-5 antibody

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor. Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.

General readings

Burnatowska-Hledin,M.A., et al., (2004) Biochem. Biophys. Res. Commun. 319 (3), 817-825

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cullin-5 antibody

  Rabbit polyclonal antibody to Cullin5 (cullin 5)  
Cullin-5 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cullin-5 (N-term) antibody

  Rabbit polyclonal anti-Cul5 antibody  
Anti-CUL5 antibody was diluted 1:500 to detect CUL5 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Rabbit Serum Hu, Ms E, IP, P, WB
0.1 ml / €356.00
  OriGene Technologies, Inc.

Cullin-5 antibody

  Anti-CUL5 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CUL5 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cullin-5 antibody

  Rabbit anti-CUL5 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CUL5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cullin-5 antibody

  Rabbit Polyclonal Anti-CUL5 Antibody  
WB Suggested Anti-CUL5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cullin-5 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Cullin-5 (C-term) antibody

Western blot analysis of anti-CUL5 Antibody (C-term) (Cat.#AP14229PU-N) in Hela cell line lysates (35ug/lane). CUL5 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CUL5 - human gene knockout kit via CRISPR

CUL5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CUL5 - human gene knockout kit via CRISPR

CUL5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CUL5 - human gene knockout kit via CRISPR

CUL5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CUL5 - human gene knockout kit via CRISPR

CUL5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cullin-5

  Purified recombinant protein of Homo sapiens cullin 5 (CUL5)  
Cullin-5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

CUL5 overexpression lysate

CUL5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CUL5 (mGFP-tagged) - Human cullin 5 (CUL5), 200 uL, >10^7 TU/mL

Lenti ORF particles, CUL5 (mGFP-tagged) - Human cullin 5 (CUL5), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, CUL5 (Myc-DDK tagged) - Human cullin 5 (CUL5) , 200 uL, >10^7 TU/mL

Lenti ORF particles, CUL5 (Myc-DDK tagged) - Human cullin 5 (CUL5) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cul5 (GFP-tagged) - Mouse cullin 5 (Cul5), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Cul5 (GFP-tagged) - Mouse cullin 5 (Cul5), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,590.00
  OriGene Technologies, Inc.

Lenti ORF particles, Cul5 (GFP-tagged) - Mouse cullin 5 (Cul5), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Cul5 (GFP-tagged) - Mouse cullin 5 (Cul5), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,550.00
  OriGene Technologies, Inc.

Lenti ORF particles, Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,430.00
  OriGene Technologies, Inc.

Lenti ORF particles, Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,390.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cul5 (GFP-tagged ORF) - Rat cullin 5 (Cul5), 200 uL, >10^7 TU/mL

Lenti ORF particles, Cul5 (GFP-tagged ORF) - Rat cullin 5 (Cul5), 200 uL, >10^7 TU/mL  
200 µl / €1,490.00
  OriGene Technologies, Inc.

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