Cullin-9 antibody

Principal name

Cullin-9 antibody

Alternative names for Cullin-9 antibody

CUL-9, H7AP1, KIAA0708, PARC, p53-associated parkin-like cytoplasmic protein, UbcH7-associated protein 1

SwissProt ID

Q80TT8 (Mouse), Q8IWT3 (Human)

Gene ID

23113 (CUL9)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of Cullin-9 antibody

The PARC protein, also known as p53-associated parkin-like cytoplasmic protein and UbcH7 associated protein 1, is a large (>250 kD) ubiquitously expressed cytoplasmic protein that contains a cullin domain and signature RING-IBR (in-between ring finger domain)-RING domains. PARC is thought to serve as a cytoplasmic anchor in p53-associated complexes thought to be critical in controlling p53 subcellular localization and function. These large complexes that contain PARC, p53 and other associated proteins are approximately 1 MD in non-stressed cells. The PARC protein has been shown to interact with a number of proteins including p53, UBCH8, ubiquitin conjugating enzyme E2G2, and ubiquitin conjugating enzyme E2L3. 
Structure: Contains a cullin domain and signature motif RING-IBR (in between ring finger domain)-RING, approximately 270 kD.
Distribution: Ubiquitously expressed cytoplasmic protein, highest expression in testis and lowest expression in thymus.
Function: Cytoplasmic protein anchor in large p53-associated protein complexes (approximately 1 MD in non-stressed cells). Thought to be a critical regulator in controlling p53 subcellular localization and function.
Interaction: p53, UBCH8, ubiquitin conjugating enzyme E2G2, ubiquitin conjugating enzyme E2L3

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cullin-9 antibody

Western blot analysis of PARC in Daudi lysate with PARC antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cullin-9 antibody

Anti-CUL9 / Cullin 9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Cullin-9 antibody

HepG2 cell extract (left lane) or NIH 3T3 cell extract (right lane) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PARC antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. In all human cell lines tested, this antibody recognizes a non-specific 66 kD protein in addition to PARC. Mouse IgG1 PO69 Aff - Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cullin-9 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Colon. Rabbit Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Cullin-9 Control Peptide

Cullin-9
50 µg / €160.00
  Acris Antibodies GmbH

Cullin-9 antibody

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid).
Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to
form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S. Jentsch, see references above. Rabbit Serum Hu, Ms E, IP, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

Cullin-9 antibody

Cullin-9 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Cullin-9 antibody

PARC monoclonal antibody (M01), clone 3F7 Western Blot analysis of PARC expression in A-431 ( Cat # L015V1 ). Mouse IgG2a 3F7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cullin-9 antibody

Immunocytochemistry of CUL9 in Daudi cells with CUL9 polyclonal antibody ( Cat # PAB12904 ) at 1 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Cullin-9 (C-term) antibody

Human testis: Formalin-Fixed Paraffin-Embedded (FFPE). Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €460.00
  Neuromics Antibodies

Cullin-9 antibody

Western Blot and Immunoprecipitation: PARC/H7-AP1 Antibody [NB100-2300] - Detection of Human PARC/H7-AP1 on HeLa whole cell lysate using NB100-2300. Normal rabbit IgG was used as a control for immunoprecipitation (IP Control). Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Cullin-9 antibody

HepG2 cell extract (left lane) or NIH 3T3 cell extract (right lane) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PARC antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. In all human cell lines tested, this antibody recognizes a non-specific 66 kD protein in addition to PARC. Mouse IgG1 PO69 Aff - Purified Hu, Ms WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Cullin-9 antibody

Western Blot and  Immunoprecipitation: PARC Antibody [NB200-187] - Detection of Human PARC on HeLa whole cell lysate using NB200-187. PARC was also immunoprecipited using NB200-186. Immunoprecipitated PARC was blotted using NB200-187. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Cullin-9 antibody

Immunohistochemistry-Paraffin: PARC Antibody [NBP1-77222] - Parc in Daudi cells with Parc antibody at 1 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cullin-9

Cullin-9 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Cullin-9

Cullin-9 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CUL9 overexpression lysate

CUL9 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

17 Item(s)

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