CYB5R3 antibody

Principal name

CYB5R3 antibody

Alternative names for CYB5R3 antibody

NADH-cytochrome b5 reductase 3, B5R, DIA1, Diaphorase-1

SwissProt ID

P00387 (Human), P07514 (Bovin), P20070 (Rat), P83686 (Pig), Q0X0E5 (Canfa), Q9DCN2 (Mouse)

Gene ID

1727 (CYB5R3), 109754 (Cyb5r3)

Available reactivities

Hst (Hamster), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of CYB5R3 antibody

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (s) is the soluble form of the enzyme, which has a shorter N-terminus when it is compared to the membrane-bound form. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Percy MJ., et al. (2006) Arch Biochem Biophys. 447(1):59-67.Roma GW., et al. (2006) Arch Biochem Biophys. 452(1):69-82.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CYB5R3 (C-term) antibody

TA305933 (0.1ug/ml) staining of Mouse Liver lysate  (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CYB5R3 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501294) Mouse IgG1 OTI1E8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CYB5R3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353) Mouse IgG2b OTI2A10 Purified Can, Hu, Mky F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CYB5R3 antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (TA501385) Mouse IgG1 OTI1E3 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CYB5R3 antibody

Immunohistochemistry (IHC) analyzes of CYB5R3 antibody (Cat.-No.: AP01486PU-N) in paraffin-embedded human placenta tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CYB5R3 antibody

CYB5R3 polyclonal antibody (A01), Lot # AUL1060606QCS1. Western Blot analysis of CYB5R3 expression in NIH/3T3. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CYB5R3 (C-term) antibody

AP23661PU-N Cyb5r3 antibody staining of Mouse Lung lysate at 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CYB5R3 (C-term) antibody

AP23662PU-N Cyb5r3 antibody staining of Human Umbilical Cord lysate at 0.01 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CYB5R3 antibody

WB Suggested Anti-CYB5R3 Antibody; Titration: 1.0 ug/ml; Positive Control: HT1080 Whole Cell Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CYB5R3 antibody

Immunohistochemistry (IHC) analyzes of Cytochrome b5 antibody (Cat.-No.: AP20483PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CYB5R3 antibody

CYB5R3 Rabbit IgG Azide Free Bov Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

CYB5R3 antibody

CYB5R3 Rabbit IgG Biotin Bov Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

CYB5R3 antibody

Immunofluorescence of purified MaxPab antibody to CYB5R3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

CYB5R3 antibody

Immunofluorescence of purified MaxPab antibody to CYB5R3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CYB5R3 antibody

CYB5R3 Mouse IgG2a 2A9 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

CYB5R3 antibody

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using CYB5R3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
50 µg / €205.00
  Assay Biotech

CYB5R3 antibody

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using CYB5R3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €310.00
  Assay Biotech

CYB5R3 (C-term) antibody

Human HT1080; WB Suggested Anti-CYB5R3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: HT1080 Whole Cell; CYB5R3 antibody - C-terminal region (ARP60822_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CYB5R3 antibody

Immunohistochemical analysis of CYB5R3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Can, Hu IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CYB5R3 antibody

Immunohistochemical analysis of CYB5R3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Can, Hu IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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