Cyclin B1 antibody

Principal name

Cyclin B1 antibody

Alternative names for Cyclin B1 antibody

CCNB1, CCNB, Cyclin-B1, G2/mitotic-specific cyclin-B1

SwissProt ID

P13350 (Xenla), P14635 (Human), P24860 (Mouse), P30277 (Rat), Q1LZG6 (Bovin)

Gene ID

891 (CCNB1), 268697 (Ccnb1), 25203

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster), Bov (Bovine), Can (Canine), Por (Porcine), Chimp (Chimpanzee), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep), Mky (Monkey), Mam (Mammalia)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP), EPair for Elisa (EPair), Flow Cytometry (F)

Background of Cyclin B1 antibody

Cyclins are evolutionarily conserved regulatory proteins that are essential for controlling the progression of the cell-cycle. Proliferating cells express both cyclin B1 and cyclin B2; expression is normally low in G0/G1, increases in S, and is maximal during the G2/M phase of cell division. Cyclin B1 can be found both on intracellular membranes and free in the cytoplasm, in contrast to cyclin B2, which is membrane-associated. Cyclin B1 binds to p34Cdc2, also known as Cdk1, a cyclin-dependent kinase, to form an inactive complex that accumulates in the cytoplasm. Dephosphorylation by Cdc25 results in activation of the complex and translocation to the nucleus. Cyclin B1 is rapidly degraded at the end of mitosis, and this degradation is essential for cells to exit from mitosis.
In mammals, Cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition. 
Structure: Belongs to the cyclin family. Cyclin AB subfamily.
Distribution: Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.
Interaction: CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4.
Bioactivity/Activities: Essential for the control of the cell cycle at the G2/M (mitosis) transition.

General readings

1. Álvarez-Fernández M  et al. Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals. Proc Natl Acad Sci U S A 110:17374-9 (2013). Mouse .
2. Zheng P & Dean J Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis. Proc Natl Acad Sci U S A 106:7473-8 (2009). ICC/IF ; Mouse .
3. Enomoto M  et al. Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during G2/M transition. J Biol Chem 284:34223-30 (2009). WB ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclin B1 antibody

Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using Cyclin B1 Antibody Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Cyclin B1 antibody

Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using Cyclin B1 Antibody Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

Cyclin B1 antibody

  Rabbit Polyclonal Cyclin B1 Antibody  
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Rabbit polyclonal Cyclin B1 (Ab-147) antibody  
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using Cyclin B1 (Ab-147) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Rabbit polyclonal Cyclin B1 (Ser147) antibody(Phospho-specific)  
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using Cyclin B1 (Phospho-Ser147) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Rabbit polyclonal Cyclin B1 (Ab-126) antibody  
Western blot analysis of extracts from HeLa cells, treated with serum (10%, 15min), using Cyclin B1 (Ab-126) antibody (#BOO68, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Rabbit polyclonal Cyclin B1 antibody  
WB analysis using anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG. Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €356.00
  OriGene Technologies, Inc.

Cyclin B1 pSer126 antibody

  Rabbit polyclonal Cyclin B1 phospho S126 antibody  
WB using Anti-Cyclin B pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B(arrowheads) in various whole cell lysates.  Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). The primary antibody was used at 1:100. HRP conjugated Gt-a-Rabbit IgG [H&L] was used at 1:5000. Rabbit Aff - Purified Hu E
0.1 mg / €356.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Anti-CCNB1 (phospho-Ser147) Rabbit Polyclonal Antibody  
Predicted band size: 60 kDa . Positive control: MDA cells untreated or treated with Anisomycin lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: MDA cells untreated with Anisomycin lysate Lane2: MDA cells treated with Anisomycin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Anti-CCNB1 Rabbit Polyclonal Antibody  
Western blot analysis of Jurkat, 231 and Hela cell using TA322150(CCNB1 Antibody) at dilution 1/300 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Anti-CCNB1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CCNB1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Rabbit polyclonal Mouse CCNB1 Antibody (Center S123/S125)  
Mouse CCNB1 Antibody (Center S123/S125)(Cat. #TA325137) western blot analysis in mouse NIH/3T3 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the mouse CCNB1 antibody detected the mouse CCNB1 protein (arrow). Rabbit IgG RB42994 Purified Hst, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Rabbit Polyclonal Cyclin B1 Antibody  
Western blot analysis of Cyclin B1 expression in UV treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Rabbit Polyclonal Cyclin B1 Antibody  
Western blot analysis of Cyclin B1 expression in serum treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Rabbit Polyclonal Cyclin B1 (Ser126) Antibody (Phospho-specific)  
Western blot analysis of Cyclin B1 phosphorylation expression in EGF treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Rabbit Polyclonal Cyclin B1 (Ser147) Antibody (Phospho-specific)  
Western blot analysis of Cyclin B1 phosphorylation expression in UV treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  CCNB1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of HepG2 cell lines, using CCNB1 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Mouse Monoclonal anti-Cyclin B1 Antibody  
Western blot analysis of extracts from Hela (lane 1) and NIH/3T3 (lane 2) cells, using anti-Cyclin B1, V152. Mouse IgG1 V152 Purified Hst, Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Mouse monoclonal Anti-CyclinB1 Clone V143.1  
Cyclin B1 Mouse IgG1 V143.1 Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

  Mouse monoclonal Anti-CyclinB1 Clone V152  
Cyclin B1 Mouse IgG1 V152 Purified Mam F, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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