Cyclin B1 antibody

Principal name

Cyclin B1 antibody

Alternative names for Cyclin B1 antibody

CCNB1, CCNB, Cyclin-B1, G2/mitotic-specific cyclin-B1

SwissProt ID

P13350 (Xenla), P14635 (Human), P24860 (Mouse), P30277 (Rat), Q1LZG6 (Bovin)

Gene ID

891 (CCNB1), 268697 (Ccnb1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster), Bov (Bovine), Can (Canine), Por (Porcine), Chimp (Chimpanzee), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Frozen Sections (C), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of Cyclin B1 antibody

Cyclins are evolutionarily conserved regulatory proteins that are essential for controlling the progression of the cell-cycle. Proliferating cells express both cyclin B1 and cyclin B2; expression is normally low in G0/G1, increases in S, and is maximal during the G2/M phase of cell division. Cyclin B1 can be found both on intracellular membranes and free in the cytoplasm, in contrast to cyclin B2, which is membrane-associated. Cyclin B1 binds to p34Cdc2, also known as Cdk1, a cyclin-dependent kinase, to form an inactive complex that accumulates in the cytoplasm. Dephosphorylation by Cdc25 results in activation of the complex and translocation to the nucleus. Cyclin B1 is rapidly degraded at the end of mitosis, and this degradation is essential for cells to exit from mitosis.
In mammals, Cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition. 
Structure: Belongs to the cyclin family. Cyclin AB subfamily.
Distribution: Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.
Interaction: CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4.
Bioactivity/Activities: Essential for the control of the cell cycle at the G2/M (mitosis) transition.

General readings

This product has been used in:Geley S et al. Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint. J Cell Biol 153:137-48 (2001). PubMed PMID: 11285280
Brandeis M et al. Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero. Proc Natl Acad Sci U S A 95:4344-9 (1998). PubMed PMID: 9539739
General / background references:Minemoto Y et al. Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53. Exp Cell Res 262:37-48 (2001). PubMed PMID: 11120603

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclin B1 antibody

Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using Cyclin B1 Antibody Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Cyclin B1 antibody

Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using Cyclin B1 Antibody Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Cyclin B1 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CCNB1 and CDC25A. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-CDC25A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Cyclin B1 antibody

Human Jurkat; WB Suggested Anti-CCNB1 Antibody Titration: 0.125ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. CCNB1 is supported by BioGPS gene expression data to be expressed in Jurkat.; CCNB1 antibody - C-terminal region (ARP30161_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Cyclin B1 antibody

Breast carcinoma stained with Anti-Cyclin B1 antibody. Rabbit Purified Hu, Ms, Rt IP, P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Cyclin B1 antibody

Breast carcinoma stained with Anti-Cyclin B1 antibody. Rabbit Purified Hu, Ms, Rt IP, P, WB
1 ml / €440.00
  Acris Antibodies GmbH

Cyclin B1 antibody

Breast carcinoma stained with Anti-Cyclin B1 antibody. Rabbit Purified Hu, Ms, Rt IP, P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

Cyclin B1 antibody

Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using Cyclin B1 (Ab-147) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Western blot analysis of extracts from HeLa cells, treated with serum (10%, 15min), using Cyclin B1 (Ab-126) antibody (#BOO68, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

WB analysis using anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG. Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 pSer126 antibody

WB using Anti-Cyclin B pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B(arrowheads) in various whole cell lysates.  Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). The primary antibody was used at 1:100. HRP conjugated Gt-a-Rabbit IgG [H&L] was used at 1:5000. Rabbit Aff - Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Western blot analysis of Jurkat, 231 and Hela cell using TA322150(CCNB1 Antibody) at dilution 1/300 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CCNB1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Mouse CCNB1 Antibody (Center S123/S125)(Cat. #TA325137) western blot analysis in mouse NIH/3T3 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the mouse CCNB1 antibody detected the mouse CCNB1 protein (arrow). Rabbit IgG RB42994 Purified Hst, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Western blot analysis of Cyclin B1 expression in UV treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Western blot analysis of Cyclin B1 expression in serum treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Western blot analysis of extracts of HepG2 cell lines, using CCNB1 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Western blot analysis of extracts from Hela (lane 1) and NIH/3T3 (lane 2) cells, using anti-Cyclin B1, V152. Mouse IgG1 V152 Purified Hst, Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin B1 antibody

Immunohistochemistry: AM00228AF-N Cyclin B1 antibody staining of  Paraffin Embedded Human Tonsil Section (germinal center). Mouse IgG1 V152 Azide Free Hst, Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

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