Cyclin B2 antibody

Principal name

Cyclin B2 antibody

Alternative names for Cyclin B2 antibody

Cyclin-B2, CCNB2

SwissProt ID

O77689 (Bovin), O95067 (Human), P13351 (Xenla), P29332 (Chick), P30276 (Mouse)

Gene ID

397743 (ccnb2), 9133 (CCNB2), 3913351 (Q2FF06), 12442

Available reactivities

Xen (Xenopus), Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Dot blot (Dot)

Background of Cyclin B2 antibody

Cyclin B2 belongs to a the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin B2 has been shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins.

General readings

This product has been used in:Moore JD et al. Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis. Mol Biol Cell 13:4388-400 (2002). PubMed PMID: 12475960
Bodart JF et al. Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis. Exp Cell Res 253:413-21 (1999). PubMed PMID: 10585264

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclin B2 antibody

Predicted cell location: Cell membrane . Positive control: Human esophagus cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CCNB2 antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B2 antibody

Predicted cell location: Cytoplasm . Positive control: Human esophagus cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CCNB2 antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin B2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB2 (RC211260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB2. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin B2 antibody

ELISA: Cyclin B2 Antibody [29000002] Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

Cyclin B2 antibody

Cyclin B2 Mouse IgG1 X121.10 Purified Xen IP, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

Cyclin B2 antibody

Cyclin B2 Mouse IgG1 X29.2 Purified Hu, Ms, Rt, Xen WB
0.1 mg / €320.00
  Novus Biologicals Inc.

Cyclin B2 antibody

Western Blot: cyclin B2 Antibody [NBP1-33031] - Western blot analysis of cyclin B2 on H1299 lysates using NBP1-33031. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Cyclin B2 antibody

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-89657] - Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cyclin B2

Cyclin B2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Cyclin B2 (1-398, His-tag)

Cyclin B2 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

Cyclin B2 (1-398, His-tag)

Cyclin B2 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

Cyclin B2

Cyclin B2 Human
50 µg / €450.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CCNB2 overexpression lysate

CCNB2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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