Cyclin D2 antibody

Principal name

Cyclin D2 antibody

Alternative names for Cyclin D2 antibody

Cyclin-D2, CCND2

SwissProt ID

P30279 (Human), P30280 (Mouse), P49706 (Chick), P53782 (Xenla), Q04827 (Rat), Q0P5D3 (Bovin), Q8WNW2 (Pig)

Gene ID

894 (CCND2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C), Functional assay (FN), Paraffin Sections (P)

Background of Cyclin D2 antibody

Cyclin D2, also known as CCND2, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1, Cyclin D2 and Cyclin D3 are the members of the cyclin family. Cyclin D2 mapped to 12p131, since the CCND1 gene is on 11q13, this may be another bit of evidence of the homology of chromosomes 11 and 12. Choi D et al proved the expression of pseudogene cyclin D2 mRNA in the human ovary increases with age, which may be a novel marker for decreased ovarian function associated with the aging process.2 And knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Inaba et al. (1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. Cyclin D2(CCND2) gene ia assigned to 12p13.

General readings

Clone DCS-5 is used in these references:
1. Kohoutek, J., et al., Biol. Reprod. 70, 139-145 (2004)
2. Oirschot, B. A., et al., J. Biol. Chem. 276, 33854-33860 (2001)
3. Tanguay, D. A., et al., J. Immunol. 166, 4273-4277 (2001)
4. Deininger, M. W. N., et al., Cancer Res. 61, 8005-8013 (2001)
5. Boonen, G. J. J., et al., J. Biol. Chem. 274, 34676-34682 (1999)
6. Tanguay, D., et al., J. Immunol. 163, 3160-3168 (1999)
7. Tanguay, D. A., et al., J. Exp. Med. 189, 1685-1690 (1999)
8. Bartkova, J., et al., Cancer Res. 56, 5475-5483 (1996)
9. Pokrovskaja, K., et al., Cell Growth Differ. 7, 1723-1732 (1996)
10. Lukas, J., et al., Oncogene 10, 2125-2134 (1995)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclin D2 antibody

Cyclin D2 Mouse IgG2a DCS-3.1 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

Cyclin D2 antibody

Western blot analysis of extracts from 293 cells, using Cyclin D2 (Ab-280) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

Predicted cell location: Nucleus . Positive control: Human esophagus cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CCND2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

Predicted band size: 33 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

Western blot detection of Cyclin D2 in CHO-K1 cell lysate(A)and CHO-K1 transfected by Cyclin D2-fragment EGFP fusion protein(B)cell lysate using Cyclin D2 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa. Mouse IgG2b 6E11-G6-F5 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

Western blot analysis of Cyclin D2 expression in 293 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI5A6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2.(1:2000) Mouse IgG1 OTI5H6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI1E4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG1 OTI7G9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI1C8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI2D6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI10E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

Figure 1. Western blot analysis of Cyclin D2 expression in NIH/3T3 cells using Cyclin D2 antibody (AM20022AF-N). Mouse IgG2a DCS-3 Azide Free Hu, Ms IP, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Cyclin D2 antibody

Figure 1. Western blot analysis of Cyclin D2 expression in NIH/3T3 cells using Cyclin D2 antibody (AM20023AF-N). Mouse IgG2b DCS-5 Azide Free Hu, Ms IP, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Cyclin D2 antibody

Cyclin D2 Mouse IgG2a CY-D2 Purified Hu, Ms ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Cyclin D2 (C-term) antibody

Cyclin D2 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Cyclin D2 antibody

Cyclin D2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Cyclin D2 antibody

CCND2 MaxPab polyclonal antibody. Western Blot analysis of CCND2 expression in human placenta. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Cyclin D2 antibody

Western Blot analysis of CCND2 expression in transfected 293T cell line (H00000894-T02) by CCND2 MaxPab polyclonal antibody.

Lane 1: CCND2 transfected lysate(33.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

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