Cyclin D2 antibody

Principal name

Cyclin D2 antibody

Alternative names for Cyclin D2 antibody

Cyclin-D2, CCND2

SwissProt ID

P30279 (Human), P30280 (Mouse), P49706 (Chick), P53782 (Xenla), Q04827 (Rat), Q0P5D3 (Bovin), Q8WNW2 (Pig)

Gene ID

894 (CCND2), 12444 (Ccnd2), 64033

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of Cyclin D2 antibody

Cyclin D2, also known as CCND2, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1, Cyclin D2 and Cyclin D3 are the members of the cyclin family. Cyclin D2 mapped to 12p131, since the CCND1 gene is on 11q13, this may be another bit of evidence of the homology of chromosomes 11 and 12. Choi D et al proved the expression of pseudogene cyclin D2 mRNA in the human ovary increases with age, which may be a novel marker for decreased ovarian function associated with the aging process.2 And knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Inaba et al. (1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. Cyclin D2(CCND2) gene ia assigned to 12p13.

General readings

Clone DCS-5 is used in these references:
1. Kohoutek, J., et al., Biol. Reprod. 70, 139-145 (2004)
2. Oirschot, B. A., et al., J. Biol. Chem. 276, 33854-33860 (2001)
3. Tanguay, D. A., et al., J. Immunol. 166, 4273-4277 (2001)
4. Deininger, M. W. N., et al., Cancer Res. 61, 8005-8013 (2001)
5. Boonen, G. J. J., et al., J. Biol. Chem. 274, 34676-34682 (1999)
6. Tanguay, D., et al., J. Immunol. 163, 3160-3168 (1999)
7. Tanguay, D. A., et al., J. Exp. Med. 189, 1685-1690 (1999)
8. Bartkova, J., et al., Cancer Res. 56, 5475-5483 (1996)
9. Pokrovskaja, K., et al., Cell Growth Differ. 7, 1723-1732 (1996)
10. Lukas, J., et al., Oncogene 10, 2125-2134 (1995)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclin D2 antibody

Cyclin D2 Mouse IgG2a DCS-3.1 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Cyclin D2 antibody

  Rabbit polyclonal Cyclin D2 (Ab-280) antibody  
Western blot analysis of extracts from 293 cells, using Cyclin D2 (Ab-280) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

  Anti-CCND2 Rabbit Polyclonal Antibody  
Predicted cell location: Nucleus . Positive control: Human esophagus cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CCND2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

  Anti-CCND2 Rabbit Polyclonal Antibody  
Predicted band size: 33 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

  Mouse Monoclonal Cyclin D2 Antibody  
Western blot detection of Cyclin D2 in CHO-K1 cell lysate(A)and CHO-K1 transfected by Cyclin D2-fragment EGFP fusion protein(B)cell lysate using Cyclin D2 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa. Mouse IgG2b 6E11-G6-F5 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

  Rabbit Polyclonal Anti-Cyclin D2 Antibody  
Western blot analysis of Cyclin D2 expression in 293 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

  Rabbit Polyclonal Anti-Cyclin D2 (Phospho-Thr280) Antibody  
Western blot analysis of Cyclin D2 (Phospho-Thr280) expression in 293 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin D2 antibody

  CCND2 mouse monoclonal antibody, clone OTI5A6 (formerly 5A6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI5A6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

  CCND2 mouse monoclonal antibody, clone OTI5H6 (formerly 5H6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2.(1:2000) Mouse IgG1 OTI5H6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

  CCND2 mouse monoclonal antibody, clone OTI1E4 (formerly 1E4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI1E4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

  CCND2 mouse monoclonal antibody, clone OTI7G9 (formerly 7G9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG1 OTI7G9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

  CCND2 mouse monoclonal antibody, clone OTI1C8 (formerly 1C8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI1C8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

  CCND2 mouse monoclonal antibody, clone OTI2D6 (formerly 2D6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI2D6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

  CCND2 mouse monoclonal antibody, clone OTI10E11 (formerly 10E11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND2 (RC210316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND2. Mouse IgG2b OTI10E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclin D2 antibody

Figure 1. Western blot analysis of Cyclin D2 expression in NIH/3T3 cells using Cyclin D2 antibody (AM20022AF-N). Mouse IgG2a DCS-3 Azide Free Hu, Ms IP, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

Cyclin D2 antibody

Figure 1. Western blot analysis of Cyclin D2 expression in NIH/3T3 cells using Cyclin D2 antibody (AM20023AF-N). Mouse IgG2b DCS-5 Azide Free Hu, Ms IP, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

Cyclin D2 antibody

Cyclin D2 Mouse IgG2a CY-D2 Purified Hu, Ms ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Cyclin D2 (C-term) antibody

Cyclin D2 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Cyclin D2 pThr280 antibody

Western blot analysis of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Cyclin D2 (Phospho-Thr280) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

Cyclin D2 pThr280 antibody

Western blot analysis of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Cyclin D2 (Phospho-Thr280) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

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