Cyclin E1 antibody

Principal name

Cyclin E1 antibody

Alternative names for Cyclin E1 antibody

Cyclin-E1, CCNE1, CCNE

SwissProt ID

P24864 (Human), P39949 (Rat), P49707 (Chick), P50756 (Xenla), Q61457 (Mouse)

Gene ID

898 (CCNE1), 12447 (Ccne1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), EPair for Elisa (EPair)

Background of Cyclin E1 antibody

Human cyclin E was originally identified by complementation of a triple cln deletion in Saccharomyces cerevisiae. Following the identification of human cyclin E protein, researchers found that breast cancers and some other solid tumors exhibited both quantitative and qualitative alterations in cyclin E protein production. In particular, the alterations in cyclin E expression in breast cancer correlated with increasing stage and grade of the tumor. These results suggested that cyclin E might be useful as a prognostic marker. More recently, Geng et al generated a mouse strain in which the coding sequences for cyclin D1 was deleted and replaced with those of human cyclin E. Replacement of cyclin D1 with cyclin E rescued all phenotypic manifestations seen in the cyclin D1 deficient mice and restored normal development to cyclin D1-dependent tissues. Based on the results of these studies, it appears that cyclin E is the major downstream target of cyclin D1. In addition to functioning downstream of cyclin D1, recent studies with Xenopus egg extracts demonstrated that cyclin E localizes to the centrosome. In these studies CDK2-cyclin E activity was shown to be required for centrosome duplication during S phase. Taken together, these data suggest a mechanism that coordinates centrosome reproduction with cycles of DNA synthesis and mitosis.

General readings

1. Murakami K. et al. J. Immunol., 161, 1694-1704 (1998).
Clone HE12 is used in this reference.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclin E1 antibody

  Rabbit polyclonal anti-Cyclin E1 antibody  
Western blot analysis of extracts from K562 cells, using Cyclin E1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Rabbit Monoclonal anti-Cyclin E1 Antibody  
Human Colon stained with anti-Cyclin E1 antibody. Rabbit SP146 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Rabbit Polyclonal Cyclin E Antibody  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CCNE1 Rabbit Polyclonal antibody. (TA590076) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Rabbit polyclonal Cyclin E1 (Thr395) antibody(Phospho-specific)  
Western blot analysis of extracts from HeLa cells, treated with Paclitasel (1uM, 60mins), using Cyclin E1 (phospho-Thr395) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Rabbit polyclonal Cyclin E1 (Ab-395) antibody  
Western blot analysis of extracts from HeLa cells, treated with Paclitasel (1uM, 60mins), using Cyclin E1 (Ab-395) antibody (#TA311854, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Anti-CCNE1 (phospho-Thr395) Rabbit Polyclonal Antibody  
Predicted band size: 47 kDa . Positive control: 293 cells treated with hydroxyurea lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: 293 cells untreated with hydroxyurea lysate Lane2: 293 cells treated with hydroxyurea lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Anti-CCNE1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm . Positive control: Human lung cancer tissue. Recommended dilution: 1/5-20  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CCNE1 antibody at dilution 1/5, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Rabbit Polyclonal Cyclin E1 Antibody  
Western blot analysis on HeLa cell lysate using Cyclin E1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Rabbit Polyclonal Cyclin E1 Antibody  
Western blot analysis of Cyclin E1 expression in Paclitaxel treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Rabbit Polyclonal Cyclin E1 Antibody  
Western Blot: Cyclin E Antibody [TA336648] - Western Blot analysis of Cyclin E1 on transfected 293T cells at 1:500 dilution. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

  Rabbit Polyclonal anti-Cyclin E Antibody  
Human Breast Carcinoma stained with anti-Cyclin E antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin E1 antibody

Cyclin E1 Mouse IgG2a 168 Purified Hu, Mky IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Cyclin E1 antibody

Immunohistochemical detection of Cyclin E on paraffin embedded section of a human lung cancer with AM20285AF-N Cyclin E antibody. Mouse IgG1 HE12 Azide Free Hu F, ICC/IF, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Cyclin E1 antibody

Immunoprecipitation of Cyclin E from K562 cells with AM20286AF-N (Lane 1) or Mouse IgG2b (Lane 2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM20286AF-N. K562 crude lysate was resolved in Lane 3. Mouse IgG2b HE172 Azide Free Hu ICC/IF, IP
0.1 mg / €320.00
  Acris Antibodies GmbH

Cyclin E1 antibody

Cyclin E1 Rabbit IgG Serum Hu, Ms, Rt E, IP, WB
50 µl / €300.00
  Acris Antibodies GmbH

Cyclin E1 antibody

Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using Cyclin E1 antibody (Cat.-No. AP06081PU-N). Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Cyclin E1 antibody

Immunohistochemical analysis in paraffin-embedded human colon carcinoma tissue using Cyclin E1 antibody (Cat.-No. AP06082PU-N). Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Cyclin E1 (C-term) antibody

Western blot analysis of Cyclin E1 (C-term) Pab in A2058 cell line lysates (35ug/lane). Cyclin E1 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Cyclin E1 (C-term) antibody

Human Testis (formalin-fixed, paraffin-embedded) stained with CCNE1 antibody AP22705PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms P
50 µg / €470.00
  Acris Antibodies GmbH

Cyclin E1 antibody

Human Lung, Respiratory Epithelium Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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