Cyclin H antibody

Principal name

Cyclin H antibody

Alternative names for Cyclin H antibody

Cyclin-H, MO15-associated protein, p37, p34, CCNH

SwissProt ID

P51946 (Human), P51947 (Xenla), Q3ZBL9 (Bovin), Q61458 (Mouse), Q9R1A0 (Rat)

Gene ID

902 (CCNH)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Por (Porcine), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of Cyclin H antibody

CCNH belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Jeang KT., et al. (1998) J Biomed Sci. 5(1): 24–27.Yankulov K., et al. (1998) Current Biology. 8(13): 447–449.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclin H antibody

Western blot analysis of extracts from K562 cells, using Cyclin H (Ab-315) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin H antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1B8 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Cyclin H antibody

Immunoperoxidase of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml] Mouse IgG2a 1B8 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cyclin H antibody

Western blot analysis of extracts of various cell lines, using CCNH antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin H antibody

WB Suggested Anti-CCNH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cyclin H antibody

WB Suggested Anti-CCNH Antibody Titration: 1ug/ml; Positive Control: Jurkat cell lysateCCNH is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin H antibody

Western blot detection of Cyclin H in Jurkat,K562 and A431 cell lysates using Cyclin H mouse mAb (1:1000 diluted).Predicted band size: 38KDa.Observed band size: 38KDa. Mouse IgG2b 4E11-G2-D7 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin H antibody

Western blot analysis on HeLa cell lysate using Cyclin H Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclin H antibody

The Recombinant Human Cyclin H (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: Recombinant Human Cyclin H
Lane 2.: HepG2 cell lysate
Lane 3.: Jurkat cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : Balb/3T3 cell lysate
Lane 6 : A431 cell lysate Mouse IgG2b AT3G6 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Cyclin H antibody

The Recombinant Human Cyclin H (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: Recombinant Human Cyclin H
Lane 2.: HepG2 cell lysate
Lane 3.: Jurkat cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : Balb/3T3 cell lysate
Lane 6 : A431 cell lysate Mouse IgG2b AT3G6 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

Cyclin H antibody

Western blot (WB) analysis of Cyclin H antibody (Cat.-No.: Ap20501PU-N) in extracts from K562 cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Cyclin H (C-term) antibody

CCNH Antibody (C-term) (Cat. #AP50815PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the CCNH antibody detected the CCNH protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Cyclin H (N-term) antibody

CCNH Antibody (N-term) (Cat. #AP50816PU-N) western blot analysis in mouse kidney tissue lysates (35µg/lane).This demonstrates the CCNH antibody detected the CCNH protein (arrow). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Cyclin H pThr315 antibody

Western blot analysis of extracts from JK cells (Lane 2), using Cyclin H (Phospho-Thr315) Antibody AP55720PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Cyclin H pThr315 antibody

Western blot analysis of extracts from JK cells (Lane 2), using Cyclin H (Phospho-Thr315) Antibody AP55720PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

Cyclin H antibody

Immunofluorescence of purified MaxPab antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Cyclin H pThr315 antibody

Western blot analysis of extracts from HepG2 cells, Jurkat cells, HuvEc cells and A549 cells, using Cyclin H (Phospho-Thr315) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Cyclin H pThr315 antibody

Western blot analysis of extracts from HepG2 cells, Jurkat cells, HuvEc cells and A549 cells, using Cyclin H (Phospho-Thr315) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Cyclin H antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Cyclin H (Ab-315) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

Cyclin H antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Cyclin H (Ab-315) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

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