Cyclin T2 antibody

Principal name

Cyclin T2 antibody

Alternative names for Cyclin T2 antibody

Cyclin-T2, CycT2, CCNT2

SwissProt ID

O60583 (Human)

Gene ID

905 (CCNT2)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Cyclin T2 antibody

Cyclin T2 is a ubiquitously expressed nuclear protein that is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns that contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Two alternatively spliced transcript variants, which encode distinct isoforms, have been described. Transcript variant a (isoform a) lacks an internal fragment, which leads to a translation frame change, when compared to variant b (isoform b). The resulting isoform a protein lacks a C-terminal 88 aa, and has an unique 21 aa C-terminus, as compared to isoform b.

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclin T2 antibody

Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA307981 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin T2 (Isoform A+B) antibody

WB using Anti-Cyclin T2 (Rabbit) to detect two major bands (arrowheads) corresponding to human Cyclin T2a and T2b as indicated. The primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L]  for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red). Rabbit IgG Serum Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclin T2 antibody

CCNT2 monoclonal antibody (M03), clone 1H3. Western Blot analysis of CCNT2 expression in Jurkat(Cat # L017V1 ). Mouse IgG2a 1H3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cyclin T2 antibody

Western blot using CCNT2 polyclonal antibody ( Cat # PAB11292 ) is shown to detect two major bands ( arrowheads ) corresponding to human CCN T2a and T2b as indicated.
Approximately 33 µg of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking the membrane with 5% BLOTTO in PBS, the membrane was probed forovernight at 4°C with the primary antibody diluted to 1 : 500 in 5% BLOTTO in PBS.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature ( 800 nm channel, green ).
Molecular weight estimation was made by comparison to prestained MW markers indicated at the right ( 700 nm channel, red ).
IRDyeTM 800 fluorescenceimage was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is atrademark of LI-COR, Inc. Rabbit Bov, Hu, Ms, Rt E, IP, WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

CRISPR

Catalog No. Species Donor Vector  

CCNT2 - human gene knockout kit via CRISPR

CCNT2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CCNT2 - human gene knockout kit via CRISPR

CCNT2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CCNT2 - human gene knockout kit via CRISPR

CCNT2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CCNT2 - human gene knockout kit via CRISPR

CCNT2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cyclin T2

Cyclin T2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Cyclin T2

Cyclin T2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CCNT2 (mGFP-tagged) - Human cyclin T2 (CCNT2), transcript variant a, 200 uL, >10^7 TU/mL

Lenti ORF particles, CCNT2 (mGFP-tagged) - Human cyclin T2 (CCNT2), transcript variant a, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, CCNT2 (Myc-DDK tagged) - Human cyclin T2 (CCNT2), transcript variant a , 200 uL, >10^7 TU/mL

Lenti ORF particles, CCNT2 (Myc-DDK tagged) - Human cyclin T2 (CCNT2), transcript variant a , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

12 Item(s)

per page
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