Cyclooxygenase 2 antibody

Principal name

Cyclooxygenase 2 antibody

Alternative names for Cyclooxygenase 2 antibody

Cyclooxygenase-2, PTGS2, COX2, PHS II, PGH synthase 2, PGHS-2, PGHS2, Prostaglandin G/H synthase 2, Prostaglandin-endoperoxide synthase 2, Prostaglandin H2 synthase 2

SwissProt ID

O02768 (Rabit), O19183 (Horse), O62698 (Bovin), P27607 (Chick), P35354 (Human), P35355 (Rat), P70682 (Cavpo), P79208 (Sheep), Q05769 (Mouse)

Gene ID

5743 (PTGS2), 19225 (Ptgs2), 29527, 443460

Available reactivities

Bov (Bovine), Eq (Equine), Hu (Human), Sh (Sheep), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), ELISA (detection) (E(detection))

Background of Cyclooxygenase 2 antibody

Cyclooxygenases-1 and 2 (COX-1 and COX-2) also known as prostaglandin endoperoxide H synthases-1 and 2 are integral membrane proteins which catalyze the first step of prostaglandins, thromboxanes and procastacyclin production from arachidonic acid. Both COX-1 and COX-2 catalyze a cyclooxygenase (bis-oxygenase) reaction in which arachidonate plus two molecules of O2 are converted to PGG2 (prostaglandin G2) and a peroxidase reaction in which PGG2 undergoes a two-electron reduction to PGH2. COX-1 and COX-2 proteins are encoded by separate genes located on different chromosomes and both enzymes are very similar in structure. They are homodimeric, heme containing, glycosylated proteins with two catalytic sites but they differ substantially in their pattern of expression and biology 1, 2. COX-1 is expressed constitutively in most tissues and at high levels in selected cells and tissues, including endothelium, monocytes, platelets, renal collecting tubules and seminal vesicles. COX-1 is thought to regulate a number of housekeeping activities involved in renal, gastrointestinal and platelet function. Inducible COX-2 is undetectable in most mammalian tissues but its expression can be induced rapidly (2-6h) in fibroblasts, endothelial cells, monocytes and ovarian follicles in response to growth factors, tumor promoters, hormones, bacterial endotoxin and cytokines. COX-2 is also expressed constitutively in specialized cell types or tissues where it plays specific functions in individual biological processes. These include reproduction, immunity, renal physiology, neurotransmissiom, bone resorption and pancreatic secretion 1, 2. The COX-1 and COX-2 enzymes are important pharmacologically as targets of nonsteroidal anti-inflammatory drugs (NSAIDs) including aspirin, ibuprofen and the new COX-2 inhibitors. Inhibition of cyclooxygenases with NSAIDs acutely reduces inflammation, pain and fever. Some methods to determine COX activity include measuring uptake of oxygen using an oxygraph, measuring the conversion of radioactive arachidonic acid, or measuring prostaglandins formed from PGH2 (such as determining PGH2 using immunoassays 3). These methods can be complex, time consuming and are prone to interferences.

General readings

General / background references:Prescott SM & Fitzpatrick FA Cyclooxygenase-2 and carcinogenesis. Biochim Biophys Acta 1470:M69-78 (2000). PubMed PMID: 10722929
Marnett LJ Cyclooxygenase mechanisms. Curr Opin Chem Biol 4:545-52 (2000). PubMed PMID: 11006543
Marnett LJ & Kalgutkar AS Cyclooxygenase 2 inhibitors: discovery, selectivity and the future. Trends Pharmacol Sci 20:465-9 (1999). PubMed PMID: 10542447
Ballif BA et al. Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein. Proc Natl Acad Sci U S A 93:5544-9 (1996). PubMed PMID: 8643612

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclooxygenase 2 antibody

AM05211PU-N COXII antibody staining of Human bladder tumor section. Mouse IgG1 AS66 Purified Hu C, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Cyclooxygenase 2 antibody

Western blot analysis of extracts from A549 cells, using Cox2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclooxygenase 2 (C-term) antibody

AM11127PU COX2 antibody staining of Human Lung. Rabbit IgG SP21 Supernatant Hu, Ms, Rt P
0.5 ml / €410.00
  Acris Antibodies GmbH

Cyclooxygenase 2 (C-term) antibody

AM11127PU COX2 antibody staining of Human Lung. Rabbit IgG SP21 Supernatant Hu, Ms, Rt P
1 ml / €590.00
  Acris Antibodies GmbH

Cyclooxygenase 2 (C-term) antibody

AM11127PU COX2 antibody staining of Human Lung. Rabbit IgG SP21 Supernatant Hu, Ms, Rt P
0.1 ml / €290.00
  Acris Antibodies GmbH

Cyclooxygenase 2 antibody

AP15503PU COX2 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu, Ms, Rt IP, P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Cyclooxygenase 2 antibody

AP15503PU COX2 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu, Ms, Rt IP, P, WB
1 ml / €440.00
  Acris Antibodies GmbH

Cyclooxygenase 2 antibody

AP15503PU COX2 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu, Ms, Rt IP, P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

Cyclooxygenase 2 (C-term) antibody

TA302755 staining (0.5µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por E, WB
20 µg / €200.00
  Acris Antibodies GmbH

Cyclooxygenase 2 (C-term) antibody

TA302755 staining (0.5µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclooxygenase 2 antibody

anti-Cox2 is shown to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (lane 1) and IL-1ß induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1’. Other detection systems will yield similar results. Rabbit Serum Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclooxygenase 2 antibody

Western blot analysis of Cox2 expression in A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclooxygenase 2 antibody

ELISA: COX2 Antibody Rabbit IgG Purified Hu, Ms E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclooxygenase 2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTGS2 (RC202245, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTGS2. Mouse IgG2a OTI4C4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclooxygenase 2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTGS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805286) Mouse IgG2b OTI5F12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclooxygenase 2 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTGS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805287) Mouse IgG1 OTI1B4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclooxygenase 2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTGS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805307) Mouse IgG2a OTI10H5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclooxygenase 2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTGS2 (RC202245, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTGS2. Mouse IgG1 OTI6D10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclooxygenase 2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTGS2 (RC202245, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTGS2. Mouse IgG1 OTI2F10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclooxygenase 2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTGS2 (RC202245, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTGS2. Mouse IgG1 OTI9H10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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