Cyclophilin-40 antibody

Principal name

Cyclophilin-40 antibody

Alternative names for Cyclophilin-40 antibody

PPIase D, Rotamase D, Cyclophilin-related protein, PPID, CYP40, CYPD, Cyclophilin D, CYP-40, 40 kDa peptidyl-prolyl cis-trans isomerase

SwissProt ID

P26882 (Bovin), Q08752 (Human), Q6DGG0 (Rat), Q9CR16 (Mouse)

Gene ID

5481 (PPID), 67738 (Ppid)

Available reactivities

Can (Canine), Hst (Hamster), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Functional assay (FN)

Background of Cyclophilin-40 antibody

Cyclophilin 40 is a widely expressed member of the cyclophilin type PPIase family, PPIase D subfamily. It contains 1 PPIase cyclophilin type domain and 3 TPR repeats. It catalyzes the cis trans isomerization of proline imidic peptide bonds in oligopeptides. and has a role in accelerating the folding of proteins. It is less sensitive to inhibition by cyclosporin A than CYP18.
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP).
Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.
CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues.

General readings

This product has been used in:Zou J et al. Repression of heat shock transcription factor HSF1 activation by HSP90 (HSP90 complex) that forms a stress-sensitive complex with HSF1. Cell 94:471-80 (1998). PubMed PMID: 9727490
Hoffmann K & Handschumacher RE Cyclophilin-40: evidence for a dimeric complex with hsp90. Biochem J 307 (Pt 1):5-8 (1995). PubMed PMID: 7717993
Owens-Grillo JK et al. The cyclosporin A-binding immunophilin CyP-40 and the FK506-binding immunophilin hsp56 bind to a common site on hsp90 and exist in independent cytosolic heterocomplexes with the untransformed glucocorticoid receptor. J Biol Chem 270:20479-84 (1995). PubMed PMID: 7657624
Milad M et al. Interaction of the progesterone receptor with binding proteins for FK506 and cyclosporin A. Mol Endocrinol 9:838-47 (1995). PubMed PMID: 7476967
General / background references:Hutchison KA et al. The 23-kDa acidic protein in reticulocyte lysate is the weakly bound component of the hsp foldosome that is required for assembly of the glucocorticoid receptor into a functional heterocomplex with hsp90. J Biol Chem 270:18841-7 (1995). PubMed PMID: 7642537
Kieffer LJ et al. Cyclophilin-40, a protein with homology to the P59 component of the steroid receptor complex. Cloning of the cDNA and further characterization. J Biol Chem 268:12303-10 (1993). PubMed PMID: 8509368
Kieffer LJ et al. Isolation and characterization of a 40-kDa cyclophilin-related protein. J Biol Chem 267:5503-7 (1992). PubMed PMID: 1544925

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclophilin-40 antibody

TA302580 (0.1µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclophilin-40 antibody

WB Suggested Anti-PPID Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: NCI-H226 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cyclophilin-40 antibody

Host:Rabbit; Target Name:PPID; Sample Tissue:Human Jurkat; Antibody Dilution: 1.0 ug/mlPPID is strongly supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cyclophilin-40 (N-term) antibody

Western blot analysis of PPID Antibody (N-term) (AP18156PU-N) in NIH-3T3 cell line lysates (35ug/lane). PPID (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Cyclophilin-40 antibody

Cyclophilin-40 Mouse Purified Hu, Ms, Rt E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Cyclophilin-40 antibody

Cyclophilin-40 Mouse Purified Hu, Rt E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Cyclophilin-40 antibody

Immunoprecipitation of PPID transfected lysate using anti-PPID MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PPID MaxPab mouse polyclonal antibody (B01) (H00005481-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Cyclophilin-40 antibody

Western Blot analysis of PPID expression in transfected 293T cell line (H00005481-T02) by PPID MaxPab polyclonal antibody.

Lane 1: PPID transfected lysate(40.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Cyclophilin-40 antibody

Cyclophilin-40 Mouse IgG2a 4C7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cyclophilin-40 antibody

293 overexpressing PPID and probed with PPID polyclonal antibody ( Cat # PAB7312 ) ( mock transfection in first lane), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Cyclophilin-40 antibody

Human NCI-H226; WB Suggested Anti-PPID Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: NCI-H226 cell lysate; PPID antibody - N-terminal region (ARP56624_P050) in Human NCI-H226 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Cyclophilin-40 antibody

Human 293T; Host:Rabbit. Target Name:PPID. Sample Tissue:Human 293T. Antibody Dilution: 1.0ug/ml.; PPID antibody - middle region (ARP56625_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Cyclophilin-40 antibody

Cyclophilin-40 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Cyclophilin-40 antibody

Western Blot: PPID Antibody [NB100-61665] - (0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Cyclophilin-40 antibody

Cyclophilin-40 Rabbit IgG Serum Hu, Rt C, EMSA, F, ICC/IF, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Cyclophilin-40 antibody

Western Blot: Cyclophilin D Antibody [NB120-3567] - Figure 1 shows a Western blot of CyPD on rat heart extract using NB120-3567.    Figure 2 shows a Western blot of CyPD on rat brain extract using NB120-3567. Rabbit IgG Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Cyclophilin-40 antibody

Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody [NBP1-85363] - Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cyclophilin-40

Cyclophilin-40 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Cyclophilin-40 (1-370, His-tag)

Cyclophilin-40 Human Purified by SDS-PAGE E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

Cyclophilin-40 (1-370, His-tag)

Cyclophilin-40 Human Purified by SDS-PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

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