Cyclophilin F antibody

Principal name

Cyclophilin F antibody

Alternative names for Cyclophilin F antibody

PPIase F, Rotamase F, PPIF, CYP3, mitochondrial Peptidyl-prolyl cis-trans isomerase

SwissProt ID

P29117 (Rat), P30404 (Bovin), P30405 (Human), Q99KR7 (Mouse)

Gene ID

10105 (PPIF), 5110105 (IPR000890), 282819 (Ppif)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Sh (Sheep), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of Cyclophilin F antibody

PPIF is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010).
He, Y., et al. Invest. Ophthalmol. Vis. Sci. 49(11):4912-4922 (2008).
Lamesch, P., et al. Genomics 89(3):307-315 (2007).
Lin, D.T., et al. J. Biol. Chem. 277(34):31134-31141 (2002).
Bowles, K.R., et al. Genomics 67(2):109-127 (2000).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cyclophilin F antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809070)(1:150) Mouse IgG1 OTI1G8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclophilin F antibody

Western blot analysis of extracts from HeLa cells, 293 cells and HepG2 cells, using PPIF antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cyclophilin F antibody

WB Suggested Anti-PPIF Antibody Titration: 5.0 ug/ml; Positive Control: 721_B cell lysate.PPIF is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ye, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cyclophilin F antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPIF (RC223397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPIF.(1:2000) Mouse IgG1 OTI1A1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclophilin F antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPIF (RC223397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPIF.(1:2000) Mouse IgG1 OTI4D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cyclophilin F antibody

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF Cat.-No. AM09068PU-S (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG2b AT1F5 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Cyclophilin F antibody

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF Cat.-No. AM09068PU-S (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG2b AT1F5 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Cyclophilin F (Center) antibody

Western blot analysis of PPIF Antibody (Center) Cat.-No  AP53411PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the PPIF antibody detected the PPIF protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Cyclophilin F antibody

Cyclophilin F Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Cyclophilin F antibody

Cell lysates of 293T and HeLa ( 20 µg ) were resolved by SDS-PAGE, transferred to NC membrane and probed with PPIF monoclonal antibody, clone AT1F5 ( Cat # MAB3214 ; 1 : 500 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT1F5 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Cyclophilin F antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PPIF Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

Cyclophilin F antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PPIF Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

Cyclophilin F antibody

Human 721_B; WB Suggested Anti-PPIF Antibody Titration: 5.0ug/ml. Positive Control: 721_B cell lysate. PPIF is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; PPIF antibody - middle region (ARP46141_T100) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rb, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

Cyclophilin F antibody

Immunofluorescent analysis of Cyclophilin F staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rb, Rt ICC/IF, IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Cyclophilin F antibody

Immunofluorescent analysis of Cyclophilin F staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rb, Rt ICC/IF, IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Cyclophilin F antibody

Immunofluorescent analysis of Cyclophilin F staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rb, Rt ICC/IF, IP, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Cyclophilin F antibody

Cyclophilin F Mouse AT1F5 Purified Hu E, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Cyclophilin F antibody

Cyclophilin F Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Cyclophilin F antibody

Cyclophilin F Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cyclophilin F

Cyclophilin F Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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