CYP27A1 antibody

Principal name

CYP27A1 antibody

Alternative names for CYP27A1 antibody

CYP27, Cytochrome P450 27, Cytochrome P-450C27/25, Sterol 26-hydroxylase, Sterol 27-hydroxylase, Vitamin D3-25-hydroxylase, 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase

SwissProt ID

P17177 (Rabit), P17178 (Rat), Q02318 (Human), Q9DBG1 (Mouse)

Gene ID

1593 (CYP27A1)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of CYP27A1 antibody

CYP27A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Li J  et al. De novo synthesis of steroids and oxysterols in adipocytes. J Biol Chem 289:747-64 (2014). WB .
2. Bergadà L  et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest 94:608-22 (2014).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CYP27A1 antibody

Immunohistochemistry (IHC) analysis of CYP27A1 antibody (Cat.-No AP06773PU-N) in paraffin-embedded Human lung carcinoma tissue. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CYP27A1 antibody

Western blot analysis of extracts from HeLa cells, using Cytochrome P450 27A1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CYP27A1 antibody

WB Suggested Anti-CYP27A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human brain Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CYP27A1 antibody

CYP27A1 MaxPab polyclonal antibody. Western Blot analysis of CYP27A1 expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CYP27A1 antibody

CYP27A1 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

CYP27A1 antibody

Western Blot analysis of CYP27A1 expression in transfected 293T cell line (H00001593-T02) by CYP27A1 MaxPab polyclonal antibody.

Lane 1: CYP27A1 transfected lysate(60.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CYP27A1 antibody

Western blot analysis of extracts from HeLa cells, using Cytochrome P450 27A1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

CYP27A1 antibody

Western blot analysis of extracts from HeLa cells, using Cytochrome P450 27A1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

CYP27A1 antibody

Human Brain; WB Suggested Anti-CYP27A1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; CYP27A1 antibody - middle region (ARP51773_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CYP27A1 antibody

Immunohistochemical analysis of Cytochrome P450 27A1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CYP27A1 antibody

Immunohistochemical analysis of Cytochrome P450 27A1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CYP27A1 antibody

Immunohistochemical analysis of Cytochrome P450 27A1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CYP27A1 antibody

CYP27A1 Rabbit Hu E, WB
50 µg / €400.00
  Novus Biologicals Inc.

CYP27A1 antibody

Immunohistochemistry-Paraffin: CYP27A1 Antibody [NB100-92422] - Paraffin-embedded human lung carcinoma tissue. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CYP27A1

CYP27A1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CYP27A1

CYP27A1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

CYP27A1

CYP27A1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

CYP27A1 (Human) IP-WB Antibody Pair

CYP27A1 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CYP27A1 293T Cell Transient Overexpression Lysate(Denatured)

CYP27A1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

CYP27A1 overexpression lysate

CYP27A1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

per page
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