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CYSLTR1 antibody

Principal name

CYSLTR1 antibody

Alternative names for CYSLTR1 antibody

Cysteinyl leukotriene receptor 1, CysLTR1, LTD4 receptor, HG55, HMTMF81, Cysteinyl leukotriene D4 receptor, G-protein coupled receptor HG55

SwissProt ID

Q2NNR5 (Cavpo), Q924T8 (Rat), Q95N02 (Pig), Q99JA4 (Mouse), Q9Y271 (Human)

Gene ID

10800 (CYSLTR1), 114099 (Cysltr1)

Ncbi ID

9606, NP_006630.1, NP_006630, NM_053641, NM_006639

Available reactivities

Hu (Human), Mky (Monkey), GP (Guinea Pig), Ms (Mouse), Rb (Rabbit), Bov (Bovine), Por (Porcine), Rt (Rat), Gorilla, Bear

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of CYSLTR1 antibody

LTC4, LTD4, and LTE4 are leukotrienes (cys-LTs) derived from arachidonic acid as a result of immune or inflammatory stimuli. The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also med cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their sigling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities. In mouse, CysLTR1 and CysLTR2 are subject to altertive splicing. CysLTR1 receptor expresses greater affinity for LTD4 and binds equally to LTC4 and LTE4 whereas CysLTR2 has greater and equal affinity towards LTC4 and LTD4 over LTE4. Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spil cord and adrel gland differs it from that of CysLTR1. Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestil, skin and immune disorders.

Western blot (WB) analysis of CysLTR1 antibody (Cat.-No.: AP01196PU-N) in extracts from COLO205 cells.

AP01196PU-N

Western blot (WB) analysis of CysLTR1 antibody (Cat.-No. AP01264PU-N) at 1/500 dilution.
Lane 1: Hela whole cell lysate.
Lane 2: HEK293T whole cell lysate.
Lane 3: Rat brain tissue lysate.

AP01264PU-N

Immunofluorescence analysis of COS7 cells, using CYSLTR1 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COLO205/HT-29/HeLa cells, using CYSLTR1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using CLTR1 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS7 cells, using CLTR1 Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of CYSLTR1 Antibody (Center) Cat.-No AP51175PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the CYSLTR1 antibody detected the CYSLTR1 protein (arrow).

AP51175PU-N

Western blot analysis of CYSLTR1 expression in A10 (A); HT29 (B) whole cell lysates.

Western blot analysis of mouse spleen lysate (Lanes 1 and 2: 49ug lysate) and Jurkat cells (Lanes 3 and 4: 54ug lysate) with anti-cysteinyl leukotriene receptor 1 antibody diluated at 1:2000. (Predicted and observed size: 39kDa)

TA310049

Western blot analysis of extracts from COS7 cells, using CLTR1 antibody.The lane on the right is treated with the synthesized peptide.

TA315107

Immunofluorescence analysis of HUVEC cells, using CLTR1 antibody.The picture on the right is treated with the synthesized peptide.

TA315107

Western blot analysis of acute monocytic leukemia THP-1 (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute T-cell leukemia Jurkat (lanes 3 and 6) human cell lysates: 1, 3. Anti-CysLTR1 (extracellular) antibody, (1:200). 4, 6. Anti-CysLTR1 (extracellular) antibody, preincubated with the control peptide antigen.

TA328673

Indirect flow cytometry analysis in live intact THP-1 (acute monocytic leukemia) cells: black line: Cells + goat-anti-rabbit-Cy5. green line: Cells + Anti-CysLTR1 (extracellular) antibody, (1:50) + goat-anti-rabbit-Cy5.

TA328673

Anti-CYSLTR1 / CYSLT1 antibody IHC of human nasal mucosa, allergic rhinitis, eosinophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340417

Anti-CYSLTR1 / CYSLT1 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340417

Anti-CYSLTR1 / CYSLT1 antibody IHC of human nasal mucosa, allergic rhinitis, eosinophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340418

Anti-CYSLTR1 / CYSLT1 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340418

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA310049	CYSLTR1 antibody
	Rabbit anti-CYSLTR1 polyclonal antibody
	Rabbit	IgG	Purified	Hu	WB	0.1 ml / €353.00
						OriGene Technologies, Inc.
TA315107	CYSLTR1 antibody
	Rabbit polyclonal anti-CLTR1 antibody
	Rabbit	IgG	Purified	Hu	E, ICC/IF, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image
TA328673	CYSLTR1 antibody
	Rabbit Polyclonal Anti-CysLTR1 (extracellular)
	Rabbit		Purified	Hu	WB	0.2 ml / €622.00
						OriGene Technologies, Inc.
+1 additional image
TA340417	CYSLTR1 (3rd extracell. dom.) antibody
	Rabbit Polyclonal Anti-CYSLTR1 Antibody (Extracellular Domain)
	Rabbit		Aff - Purified	GP, Hu, Ms, Rb	P	50 µg / €400.00
						OriGene Technologies, Inc.
TA340418	CYSLTR1 (C-term) antibody
	Rabbit Polyclonal Anti-CYSLTR1 Antibody (C-Terminus)
	Rabbit		Aff - Purified	Bear, Bov, Elephant, Gorilla, Hu, Mky, Ms, Por, Rb, Rt	P	50 µg / €400.00
						OriGene Technologies, Inc.
AP01196PU-N	CYSLTR1 antibody
	Rabbit		Aff - Purified	Hu	ICC/IF, P, WB	0.1 mg / €350.00
						Acris Antibodies GmbH
AP01264PU-N	CYSLTR1 antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, WB	0.1 mg / €350.00
						Acris Antibodies GmbH
AP51175PU-N	CYSLTR1 (Center) antibody
	Rabbit	Ig	Aff - Purified	Hu	WB	0.4 ml / €370.00
						Acris Antibodies GmbH
G074-1	CYSLTR1 antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, WB	50 µg / €230.00
						Assay Biotech
+1 additional image
G074-2	CYSLTR1 antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, WB	0.1 mg / €330.00
						Assay Biotech
+1 additional image
G224-1	CYSLTR1 antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, WB	50 µg / €230.00
						Assay Biotech
+1 additional image
G224-2	CYSLTR1 antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, WB	0.1 mg / €330.00
						Assay Biotech
+1 additional image

Human Lenti ORF Particles

Catalog No.
RC207763L2V	Lenti ORF particles, CYSLTR1 (mGFP-tagged) - Human cysteinyl leukotriene receptor 1 (CYSLTR1), 200 uL, >10^7 TU/mL
		200 µl / €1,082.00
		OriGene Technologies, Inc.

RC207763L1V	Lenti ORF particles, CYSLTR1 (Myc-DDK tagged) - Human cysteinyl leukotriene receptor 1 (CYSLTR1) , 200 uL, >10^7 TU/mL
		200 µl / €969.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR212803L2V	Lenti ORF particles, Cysltr1 (GFP-tagged ORF) - Rat cysteinyl leukotriene receptor 1 (Cysltr1), 200 uL, >10^7 TU/mL
		200 µl / €907.00
		OriGene Technologies, Inc.

RR212803L1V	Lenti ORF particles, Cysltr1 (Myc-DDK-tagged ORF) - Rat cysteinyl leukotriene receptor 1 (Cysltr1), 200 uL, >10^7 TU/mL
		200 µl / €804.00
		OriGene Technologies, Inc.

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