Cystatin 8 antibody

Principal name

Cystatin 8 antibody

Alternative names for Cystatin 8 antibody

CST8, CRES, Cystatin-related epididymal spermatogenic protein

SwissProt ID

O60676 (Human), O88969 (Rat), P32766 (Mouse)

Gene ID

10047 (CST8)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of Cystatin 8 antibody

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteise inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Altertive splicing identified in mouse is suggested in human based on EST evidence but the full-length ture of putative variants has not been determined. [provided by RefSeq, Jul 2008]

General readings

1. Chapuis J, Hot D, Hansmannel F, Kerdraon O, Ferreira S, Hubans C, Maurage CA, Huot L, Bensemain F, Laumet G, Ayral AM, Fievet N, Hauw JJ, DeKosky ST, Lemoine Y, Iwatsubo T, Wavrant-Devrièze F, Dartigues JF, Tzourio C, Buée L, Pasquier F, Berr C, Mann D, Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer's disease. Molecular psychiatry 2009 Nov 14 (11): 1004-16.
PMID: 19204726

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cystatin 8 (23-34) antibody

HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human CST8 with C-terminal MYC tag probed with AP21268PU-N  (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in Lane C. Mock-transfected HEK294 probed with AP21271PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Cystatin 8 (100-112) antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 (RC210130) with C-terminal MYC tag probed with TA311332 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in Lane C. Mock-transfected HEK293 probed with TA311332 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin 8 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue using CST8 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cystatin 8 antibody

Predicted band size: 16 kDa . Positive control: Testis tissue, HEK-293 and DU145 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Testis tissue lysate Lane2: HEK-293 cell lysate Lane3: DU145 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cystatin 8 antibody

Western blot analysis of extracts of various cell lines, using CST8 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin 8 antibody

WB Suggested Anti-CST8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cystatin 8 antibody

Cystatin 8 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Cystatin 8 antibody

Western Blot analysis of CST8 expression in transfected 293T cell line (H00010047-T01) by CST8 MaxPab polyclonal antibody.

Lane 1: CST8 transfected lysate(9.9 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Cystatin 8 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CST8 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

Cystatin 8 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CST8 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

Cystatin 8 antibody

Human THP-1; WB Suggested Anti-CST8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: THP-1 cell lysate; CST8 antibody - middle region (ARP52079_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Cystatin 8 (N-term) antibody

Cystatin 8 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Cystatin 8 antibody

Cystatin 8 Rabbit Purified Hu C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Cystatin 8 antibody

Western Blot: Cystatin-8 Antibody [NBP1-57663] - This Anti-CST8 antibody was used in Western Blot of THP-1       tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Cystatin 8 antibody

Immunohistochemistry-Paraffin: Cystatin-8 Antibody [NBP1-85456] Immunohistochemical staining of human epididymis shows strong cytoplasmic and membranous positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cystatin 8

Cystatin 8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Cystatin 8

Cystatin 8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Cystatin 8

Cystatin 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Cystatin 8

Cystatin 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CST8 293T Cell Transient Overexpression Lysate(Denatured)

CST8 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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