Cystatin-C antibody

Principal name

Cystatin-C antibody

Alternative names for Cystatin-C antibody

Cystatin C, Cystatin-3, CST3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin

SwissProt ID

O97862 (Rabit), P01034 (Human), P01035 (Bovin), P14841 (Rat), P21460 (Mouse)

Gene ID

1471 (CST3), 13010 (Cst3), 25307

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Dros (Drosophila), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Luminex (LMNX)

Background of Cystatin-C antibody

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. (provided by RefSeq)Tissue specificity: Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.

General readings

1. Danjo A, Yamaza T, Kido MA, Shimohira D, Tsukuba T, Kagiya T, Yamashita Y, Nishijima K, Masuko S, Goto M, Tanaka T. Cystatin C stimulates the differentiation of mouse osteoblastic cells and bone formation. Biochem Biophys Res Commun. 2007, Aug 17;360(1):199-204. PubMed PMID: 17592728.
2. Nedelkov D, Nelson RW . Delineating protein-protein interactions via biomolecular interaction analysis-mass spectrometry. J Mol Recognit. 2003, Jan-Feb;16(1):9-14. PubMed PMID: 12557233. 
3. Nedelkov D, Nelson RW . Design and use of multi-affinity surfaces in biomolecular interaction analysis-mass spectrometry (BIA/MS): a step toward the design of SPR/MS arrays. J Mol Recognit. 2003, Jan-Feb;16(1):15-9. PubMed PMID: 12557234.
4. Konduri SD, Yanamandra N, Siddique K, Joseph A, Dinh DH, Olivero WC, Gujrati M, Kouraklis G, Swaroop A, Kyritsis AP, Rao JS . Modulation of cystatin C expression impairs the invasive and tumorigenic potential of human glioblastoma cells. Oncogene 2002, Dec 12;21(57):8705-12. PubMed PMID: 12483523.
5. Page M.K. et al. Clinical value of cystatin C determination. Clinica Chim. Acta 297, 67-72 (2000). PubMed PMID: 10841909.
6. Pirttila TJ, Pitkanen A. Cystatin C expression is increased in the hippocampus following photothrombotic stroke in rat. Neurosci Lett. 2006 Mar 6;395(2):108-13. pub 2005 Nov 23. PubMed PMID: 16309830. 
7. Bokenkamp A, Ciarimboli G, Dieterich C. Cystatin C in a rat model of end-stage renal failure. Ren Fail. 2001 May-Jul;23(3-4):431-8. PubMed PMID: 11499558.
8. Nagai A, Ryu JK, Kobayash S, Kim SU. Cystatin C induces neuronal cell death in vivo. Ann N Y Acad Sci. 2002 Nov;977:315-21. PubMed PMID: 12480767.
9. Xu L, Sheng J, Tang Z, Wu X, Yu Y, Guo H, Shen Y, Zhou C, Paraoan L, Zhou J. Cystatin C prevents degeneration of rat nigral dopaminergic neurons: in vitro and in vivo studies. Neurobiol Dis. 2005 Feb;18(1):152-65. PubMed PMID: 15649706.
10. Price Ch.P., Finney H. Developments in the assessment of glomerular filtration rate. Clinica Chimica Acta 297, 55-66 (2000). PubMed PMID: 10841908. 
11. Ono S. et al.: . Increased cystatin C immunoreactivity in the skin in amyotrophic lateral sclerosis. Acta Neurol Scand. 102, 47-52 (2000). PubMed PMID: 10893063.
12. Ono S. et al. Increased cystatin C immunoreactivity in the skin in amyotrophic lateral sclerosis. Nicklin M.J.H. and Barrett A.J.: . Inhibition of cysteine proteinases and dipeptidyl peptidase 1 by eggwhite cystatin. Biochem J. 223, 245-253 (1984). PubMed PMID: 6388564.
13. Nicklin M.J.H. and Barrett A.J. Inhibition of cysteine proteinases and dipeptidyl peptidase 1 by eggwhite cystatin. Biochem J. 223, 245-253 (1984). PubMed PMID: 6388564.
14. Lindahl P., Abrahamson M., Bjork L. Interaction of recombinant human cystatin c with the cysteine proteinase papain and actinidin. Biochem. J. 281, 49-55 (1996). PubMed PMID: 1731767.
15. Roald AB, Aukland K, Tenstad O. Tubular absorption of filtered cystatin-C in the rat kidney. Exp Physiol. 2004 Nov;89(6):701-7. Epub 2004 Aug 24. PubMed PMID: 15328307.

  1. Quick-Filters:
  2. Highly Validated (1)
  3. Overnight Delivery (1)

Items 1 to 20 of 99 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cystatin-C antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504442) Mouse IgG1 OTI2C8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cystatin-C antibody

TA302790 staining (2µg/ml) of mouse kidney lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

TA305611 (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

Western blot analysis of extracts from COS-7 cells, using CST3 Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

Predicted band size: 16 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/2400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

Predicted band size: 16 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 80 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

Cystatin-C Mouse IgG1 6F12-C7-D8 Purified
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

Cystatin-C Mouse IgG1 7F6-A5-F3 Purified
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CST3 (RC210730, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CST3. Mouse IgG1 OTI2C11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cystatin-C antibody

Immunofluorescent staining of HeLa cells using anti-CST3 mouse monoclonal antibody (TA504494). Mouse IgG1 OTI1A6 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Cystatin-C antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CST3 (RC210730, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CST3. Mouse IgG1 OTI7G8 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cystatin-C antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CST3 (RC210730, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CST3. Mouse IgG1 OTI7B8 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cystatin-C antibody

ELISA: Cystatin C Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

CST3 Luminex Elisa with 2C8 Capture (TA600251) and Anti-Tag 1B12 Detection (TA700050) Antibodies. Substrate used: Recombinant Human CST3 (TP310730) Mouse IgG1 OTI2C8 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

CST3 Luminex Elisa with 2C11 Capture (TA600252) and Anti-Tag 1B12 Detection (TA700050) Antibodies. Substrate used: Recombinant Human CST3 (TP310730) Mouse IgG1 OTI2C11 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

CST3 Luminex Elisa with 1A6 Capture (TA600253) and Anti-Tag 1B12 Detection (TA700050) Antibodies. Substrate used: Recombinant Human CST3 (TP310730) Mouse IgG1 OTI1A6 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

CST3 Luminex Elisa with 7G8 Capture (TA600254) and Anti-Tag 1B12 Detection (TA700050) Antibodies. Substrate used: Recombinant Human CST3 (TP310730) Mouse IgG1 OTI7G8 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

CST3 Luminex Elisa with 7B8 Capture (TA600255) and Anti-Tag 1B12 Detection (TA700050) Antibodies. Substrate used: Recombinant Human CST3 (TP310730) Mouse IgG1 OTI7B8 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cystatin-C antibody

CST3 Luminex Elisa with Anti-Tag 1B12 Capture (TA600050) and 2C8 Detection (TA700251) Antibodies. Substrate used: Recombinant Human CST3 (TP310730) Mouse IgG1 OTI2C8 Purified Hu LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Cystatin-C antibody

CST3 Luminex Elisa with Anti-Tag 1B12 Capture (TA600050) and 2C11 Detection (TA700252) Antibodies. Substrate used: Recombinant Human CST3 (TP310730) Mouse IgG1 OTI2C11 Purified Hu LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Items 1 to 20 of 99 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn