Cytochrome b-245 heavy chain antibody
Principal name
Cytochrome b-245 heavy chain antibody
Alternative names for Cytochrome b-245 heavy chain antibody
gp91phox, CGD91-phox, gp91-phox, gp91-1, NADPH oxidase 2, NOX2, Cytochrome b558 subunit beta, p22 phagocyte B-cytochrome, Neutrophil cytochrome b 91 kDa polypeptide, Heme-binding membrane glycoprotein, Superoxide-generating NADPH oxidase heavy chain subunit
Ncbi ID
9606, NP_000388, NP_000388.2, NM_000397, NP_031833.3, NM_007807
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)
Background of Cytochrome b-245 heavy chain antibody
The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91-kD glycoprotein (heavy chain) and a 22-kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. NADPH oxidase is a central well characterized component of the innate immune response against fungal and bacterial infections. Recent research also offers new insight into a novel role for this oxidase in the coordination of the adaptive immune response. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Structure: 570 amino acids with predicted molecular weight of 65 kD.
Distribution: Membrane, multi-pass membrane protein.
Function: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Interaction: Composed of a heavy chain (beta) and a light chain (alpha).
General readings
1. Dinauer MC, et al. 1990. J. Clin. Invest. 86:1729.
2. Shimo-Nakanishi Y, et al. 2004. Atherosclerosis 175:109.
3. Nakano Y, et al. 2008. J. Clin. Invest. 118:1176.
4. Kou B, et al. 2009. Microvasc Res. 77:125.
5. Yazdanpanah B, et al. 2009. Nature 460:1159.
6. Purushothaman D, et al. 2009. J. Exp. Med. 206:1515.
7. Yan CS, et al. 2009. J. Immunol. 182:3696.
Application References:
1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.






Primary Antibodies
Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
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TA305618 | Cytochrome b-245 heavy chain antibody |
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Goat Anti-CYBB / GP91-PHOX Antibody | |||||||
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Goat | Aff - Purified | Can, Hu | E, P, WB |
0.1 mg /
€360.00
|
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OriGene Technologies, Inc. | |||||||
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TA327116 | Cytochrome b-245 heavy chain antibody |
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Rabbit anti-CYBB Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
OriGene Technologies, Inc. | |||||||
TA328086 | Cytochrome b-245 heavy chain antibody |
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Mouse Monoclonal anti-Cytochrome b-245 heavy chain Antibody | |||||||
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Mouse | IgG1 | 54.1 | Purified | Hu, Ms | E, F, ICC/IF, WB |
0.1 mg /
€335.00
|
OriGene Technologies, Inc. | |||||||
TA335229 | Cytochrome b-245 heavy chain antibody |
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Rabbit Polyclonal Anti-CYBB Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, Hu, Ms, Rb, Rt | P, WB |
50 µg /
€360.00
|
|
OriGene Technologies, Inc. | |||||||
TA337434 | Cytochrome b-245 heavy chain antibody |
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Rabbit Polyclonal Anti-CYBB Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
€360.00
|
|
OriGene Technologies, Inc. | |||||||
TA349081 | Cytochrome b-245 heavy chain antibody |
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Rabbit Polyclonal Anti-NOX2 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€382.00
|
|
OriGene Technologies, Inc. | |||||||
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TA351456 | Cytochrome b-245 heavy chain antibody |
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Rabbit Polyclonal Anti-CYBB Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
OriGene Technologies, Inc. | |||||||
AM32453PU-N | Cytochrome b-245 heavy chain antibody |
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Mouse | IgG1 | 48 | Purified | Hu | P, WB |
0.25 mg /
€380.00
|
Acris Antibodies GmbH | |||||||
AP23109PU-N | Cytochrome b-245 heavy chain (151-163) antibody |
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Goat | Aff - Purified | Elephant, Hu | P |
50 µg /
€380.00
|
||
Acris Antibodies GmbH |
CRISPR
Catalog No. | Species | Donor Vector | |
---|---|---|---|
KN207544 | CYBB - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN207544BN | CYBB - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN207544LP | CYBB - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN207544RB | CYBB - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN304075 | Cybb - mouse gene knockout kit via CRISPR |
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Mouse | GFP-Puro |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN304075BN | Cybb - mouse gene knockout kit via CRISPR |
||
![]() |
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN304075LP | Cybb - mouse gene knockout kit via CRISPR |
||
![]() |
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN304075RB | Cybb - mouse gene knockout kit via CRISPR |
||
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Mouse | RFP-BSD |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
Catalog No. | ||
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MR208975L2V | Lenti ORF particles, Cybb (GFP-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb), 200 uL, >10^7 TU/mL |
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200 µl /
€1,257.00
|
|
OriGene Technologies, Inc. | ||
MR208975L1V | Lenti ORF particles, Cybb (Myc-DDK-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb), 200 uL, >10^7 TU/mL |
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200 µl /
€1,123.00
|
|
OriGene Technologies, Inc. | ||