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Cytochrome b-245 heavy chain antibody

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Principal name

Cytochrome b-245 heavy chain antibody

Alternative names for Cytochrome b-245 heavy chain antibody

gp91phox, CGD91-phox, gp91-phox, gp91-1, NADPH oxidase 2, NOX2, Cytochrome b558 subunit beta, p22 phagocyte B-cytochrome, Neutrophil cytochrome b 91 kDa polypeptide, Heme-binding membrane glycoprotein, Superoxide-generating NADPH oxidase heavy chain subunit

SwissProt ID

O46522 (Bovin), P04839 (Human), P52649 (Pig), Q61093 (Mouse)

Gene ID

1536 (CYBB), 13058 (Cybb)

Ncbi ID

9606, NP_000388, NP_000388.2, NM_000397, NP_031833.3, NM_007807

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bt (Bat), Do (Dolphin), Mky (Monkey), Prim (Primate), Por (Porcine), Sh (Sheep), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of Cytochrome b-245 heavy chain antibody

The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91-kD glycoprotein (heavy chain) and a 22-kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. NADPH oxidase is a central well characterized component of the innate immune response against fungal and bacterial infections. Recent research also offers new insight into a novel role for this oxidase in the coordination of the adaptive immune response. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Structure: 570 amino acids with predicted molecular weight of 65 kD.
Distribution: Membrane, multi-pass membrane protein.
Function: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Interaction: Composed of a heavy chain (beta) and a light chain (alpha).

General readings

1. Dinauer MC, et al. 1990. J. Clin. Invest. 86:1729.
2. Shimo-Nakanishi Y, et al. 2004. Atherosclerosis 175:109.
3. Nakano Y, et al. 2008. J. Clin. Invest. 118:1176.
4. Kou B, et al. 2009. Microvasc Res. 77:125.
5. Yazdanpanah B, et al. 2009. Nature 460:1159.
6. Purushothaman D, et al. 2009. J. Exp. Med. 206:1515.
7. Yan CS, et al. 2009. J. Immunol. 182:3696. 

Application References: 
1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.

ARP44276_P050 - Cytochrome b-245 heavy chain
Human Lung; . IHC Information: Lung, Human: Formalin-Fixed, Paraffin-Embedded (FFPE).; CYBB antibody - C-terminal region (ARP44276_P050) in Human Lung cells using Immunohistochemistry
AP23109PU-N - Cytochrome b-245 heavy chain
Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM32453PU-N - Cytochrome b-245 heavy chain
Formalin-Fixed, Paraffin-Embedded Human Tonsil tissue stained with NOX2 antibody CAt.-No AM32453PU-N at 10 µg/ml after heat-induced antigen retrieval.
AM33064PU-S - Cytochrome b-245 heavy chain
Extracts of human neutrophils were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-gp91phox antibody (clone 54.1). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
CPA1306-100ul - Cytochrome b-245 heavy chain
Western blot analysis of gp91 phox expression in HepG2 (A); Caco2 (B); MCF7 (C) whole cell lysates.
TA305618 - Cytochrome b-245 heavy chain
TA305618 (0.03 ug/ml) staining of Human Peripheral Blood Mononucleocytes lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA305618 - Cytochrome b-245 heavy chain
TA305618 (2 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
TA327116 - Cytochrome b-245 heavy chain
Western blot analysis of extracts of MCF7 cell lines, using CYBB antibody.
TA335229 - Cytochrome b-245 heavy chain
IHC Information: Lung, Human: Formalin-Fixed, Paraffin-Embedded (FFPE);
TA337434 - Cytochrome b-245 heavy chain
Host: Rabbit; Target Name: CYBB; Sample Tissue: 721_B Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
TA349081 - Cytochrome b-245 heavy chain
Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 ug/mL.
TA349081 - Cytochrome b-245 heavy chain
Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 ug/mL.
TA349081 - Cytochrome b-245 heavy chain
Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 ug/mL.

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cytochrome b-245 heavy chain antibody
  Goat Anti-CYBB / GP91-PHOX Antibody  
TA305618 (0.03 ug/ml) staining of Human Peripheral Blood Mononucleocytes lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.
+1 additional image

Cytochrome b-245 heavy chain antibody
  Rabbit anti-CYBB Polyclonal Antibody  
Western blot analysis of extracts of MCF7 cell lines, using CYBB antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody
  Mouse Monoclonal anti-Cytochrome b-245 heavy chain Antibody  
Extracts of human neutrophils were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-gp91phox antibody (clone 54.1). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 54.1 Purified Hu, Ms E, F, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody
  Rabbit Polyclonal Anti-CYBB Antibody  
IHC Information: Lung, Human: Formalin-Fixed, Paraffin-Embedded (FFPE); Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody
  Rabbit Polyclonal Anti-CYBB Antibody  
Host: Rabbit; Target Name: CYBB; Sample Tissue: 721_B Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody
  Rabbit Polyclonal Anti-NOX2 Antibody  
Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.
+2 additional images

Cytochrome b-245 heavy chain antibody
  Rabbit Polyclonal Anti-CYBB Antibody  
Cytochrome b-245 heavy chain Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody
Formalin-Fixed, Paraffin-Embedded Human Tonsil tissue stained with NOX2 antibody CAt.-No AM32453PU-N at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG1 48 Purified Hu P, WB
0.25 mg / €470.00
  Acris Antibodies GmbH

Cytochrome b-245 heavy chain (151-163) antibody
Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Elephant, Hu P
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CYBB - human gene knockout kit via CRISPR
CYBB - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CYBB - human gene knockout kit via CRISPR
CYBB - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CYBB - human gene knockout kit via CRISPR
CYBB - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CYBB - human gene knockout kit via CRISPR
CYBB - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cybb - mouse gene knockout kit via CRISPR
Cybb - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cybb - mouse gene knockout kit via CRISPR
Cybb - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cybb - mouse gene knockout kit via CRISPR
Cybb - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cybb - mouse gene knockout kit via CRISPR
Cybb - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

CYBB overexpression lysate
CYBB overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cybb (GFP-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb), 200 uL, >10^7 TU/mL
Lenti ORF particles, Cybb (GFP-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb), 200 uL, >10^7 TU/mL  
200 µl / €1,220.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Cybb (Myc-DDK-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb), 200 uL, >10^7 TU/mL
Lenti ORF particles, Cybb (Myc-DDK-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb), 200 uL, >10^7 TU/mL  
200 µl / €1,090.00
  OriGene Technologies, Inc.
 

20 Item(s)

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Target
  1. Cytochrome b-245 heavy chain (9)
Property
  1. 151-163 (1)
Reactivity
  1. Human (Hu) (9)
  2. Mouse (Ms) (6)
  3. Rat (Rt) (4)
  4. Canine (Can) (3)
  5. Bovine (Bov) (2)
  6. Equine (Eq) (2)
  7. Rabbit (Rb) (2)
  8. Elephant (1)
  9. Guinea Pig (GP) (1)
  10. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (6)
  3. Enzyme Immunoassay (E) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
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