Cytochrome b-245 heavy chain antibody

Principal name

Cytochrome b-245 heavy chain antibody

Alternative names for Cytochrome b-245 heavy chain antibody

gp91phox, CGD91-phox, gp91-phox, gp91-1, NADPH oxidase 2, NOX2, Cytochrome b558 subunit beta, p22 phagocyte B-cytochrome, Neutrophil cytochrome b 91 kDa polypeptide, Heme-binding membrane glycoprotein, Superoxide-generating NADPH oxidase heavy chain subunit

SwissProt ID

O46522 (Bovin), P04839 (Human), P52649 (Pig), Q61093 (Mouse)

Gene ID

1536 (CYBB)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bt (Bat), Do (Dolphin), Mky (Monkey), Prim (Primate), Por (Porcine), Sh (Sheep), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Cytochrome b-245 heavy chain antibody

The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91-kD glycoprotein (heavy chain) and a 22-kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. NADPH oxidase is a central well characterized component of the innate immune response against fungal and bacterial infections. Recent research also offers new insight into a novel role for this oxidase in the coordination of the adaptive immune response. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Structure: 570 amino acids with predicted molecular weight of 65 kD.
Distribution: Membrane, multi-pass membrane protein.
Function: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Interaction: Composed of a heavy chain (beta) and a light chain (alpha).

General readings

1. Dinauer MC, et al. 1990. J. Clin. Invest. 86:1729.
2. Shimo-Nakanishi Y, et al. 2004. Atherosclerosis 175:109.
3. Nakano Y, et al. 2008. J. Clin. Invest. 118:1176.
4. Kou B, et al. 2009. Microvasc Res. 77:125.
5. Yazdanpanah B, et al. 2009. Nature 460:1159.
6. Purushothaman D, et al. 2009. J. Exp. Med. 206:1515.
7. Yan CS, et al. 2009. J. Immunol. 182:3696. 

Application References: 
1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cytochrome b-245 heavy chain antibody

TA305618 (0.03 ug/ml) staining of Human Peripheral Blood Mononucleocytes lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody

Western blot analysis of extracts of MCF7 cell lines, using CYBB antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody

Extracts of human neutrophils were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-gp91phox antibody (clone 54.1). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 54.1 Purified Hu, Ms E, F, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody

IHC Information: Lung, Human: Formalin-Fixed, Paraffin-Embedded (FFPE); Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody

Host: Rabbit; Target Name: CYBB; Sample Tissue: 721_B Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody

Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome b-245 heavy chain antibody

Formalin-Fixed, Paraffin-Embedded Human Tonsil tissue stained with NOX2 antibody CAt.-No AM32453PU-N at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG1 48 Purified Hu P, WB
0.25 mg / €470.00
  Acris Antibodies GmbH

Cytochrome b-245 heavy chain antibody

Extracts of human neutrophils were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-gp91phox antibody (clone 54.1). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 54.1 Purified Hu, Ms E, F, ICC/IF, WB
25 µg / €240.00
  Acris Antibodies GmbH

Cytochrome b-245 heavy chain (151-163) antibody

Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bt, Can, Do, Elephant, Hu, Mky, Prim P
50 µg / €470.00
  Acris Antibodies GmbH

Cytochrome b-245 heavy chain antibody

Cytochrome b-245 heavy chain Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Cytochrome b-245 heavy chain antibody

Human Lung; . IHC Information: Lung, Human: Formalin-Fixed, Paraffin-Embedded (FFPE).; CYBB antibody - C-terminal region (ARP44276_P050) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Cytochrome b-245 heavy chain antibody

Cytochrome b-245 heavy chain Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Cytochrome b-245 heavy chain antibody

Western blot analysis of gp91 phox expression in HepG2 (A); Caco2 (B); MCF7 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Por, Rb, Rt, Sh WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Cytochrome b-245 heavy chain antibody

Western blot analysis of gp91 phox expression in HepG2 (A); Caco2 (B); MCF7 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Por, Rb, Rt, Sh WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Cytochrome b-245 heavy chain antibody

Western blot analysis of gp91 phox expression in HepG2 (A); Caco2 (B); MCF7 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Por, Rb, Rt, Sh WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Cytochrome b-245 heavy chain antibody

NOX2/gp91phox Antibody [CL5] Mouse IgG1 CL5 Purified Hu WB
0.1 ml / €400.00
  Immuquest

Cytochrome b-245 heavy chain antibody

NOX2/gp91phox Antibody [NL7] Mouse IgG1 NL7 Purified Hu WB
0.1 ml / €400.00
  Immuquest

Cytochrome b-245 heavy chain antibody

NOX2/gp91phox Antibody [54.1] Mouse IgG1 54.1 Purified Hu ICC/IF, P, WB
0.1 ml / €400.00
  Immuquest

Cytochrome b-245 heavy chain antibody

Cytochrome b-245 heavy chain Mouse IgG1 NL7 FITC Hu F
50 µg / €310.00
  Novus Biologicals Inc.

Cytochrome b-245 heavy chain antibody

Cytochrome b-245 heavy chain Mouse IgG1 NL7 Purified Hu C, E, F, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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