Cytochrome c antibody

Principal name

Cytochrome c antibody

Alternative names for Cytochrome c antibody

CYCS, CYC

SwissProt ID

P00004 (Horse), P00011 (Canfa), P62894 (Bovin), P62897 (Mouse), P62898 (Rat), P99999 (Human)

Gene ID

54205 (CYCS), 13063 (Cycs), 100363502 (LOC100363502), 100847700 (LOC100847700)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Dros (Drosophila), Hst (Hamster), Pigeon, Por (Porcine), Gt (Goat), Mky (Monkey), Sh (Sheep), Bov (Bovine), Eq (Equine), Can (Canine), Rb (Rabbit), Chk (Chicken), Mam (Mammalia), Prim (Primate)

Available hosts

Mouse, Rabbit, Chicken, Sheep

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Frozen Sections (C), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R), Functional assay (FN)

Background of Cytochrome c antibody

Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells. Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria. Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Moreover, elevated cytochrome c levels were observed in serum from patients with hematological malignancies. In the course of cancer chemotherapy, the serum-cytochrome c level grew rapidly and it decreased gradually as the patient was cleared from malignant cells. Thus, serum-cytochrome c monitoring might serve as a clinical marker indicating the onset of apoptosis and cell turn-over in vivo.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cytochrome c antibody

HeLa  cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 ug/ml of purified cytochrome c (clone 6H2.B4) in blocking buffer overnight at 4°C, and followed by DyLight™ 594 anti-mouse IgG (red) staining for 2 hours at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective. Mouse IgG1 6H2.B4 Purified Hu, Ms, Rt F, FN, ICC/IF, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Cytochrome c Mouse IgG1 6H2.B4 Biotin Hu, Ms, Rt F, ICC/IF, IP
0.1 mg / €320.00
  Acris Antibodies GmbH

Cytochrome c antibody

Balb/c splenocytes intracellularly stained with FITC conjugated Cytochrome c Antibody Clone 6H2.B4. Mouse IgG1 6H2.B4 FITC Hu, Ms, Rt F
0.1 mg / €360.00
  Acris Antibodies GmbH

Cytochrome c antibody

HeLa  cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 ug/ml of purified cytochrome c (clone 6H2.B4) in blocking buffer overnight at 4°C, and followed by DyLight™ 594 anti-mouse IgG (red) staining for 2 hours at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective. Mouse IgG1 6H2.B4 Purified Hu, Ms, Rt F, FN, ICC/IF, IP
25 µg / €190.00
  Acris Antibodies GmbH

Cytochrome c antibody

Western blot analysis of extracts of mouse kidney cell line, using CYCS antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Western blot analysis of extracts from COS7 cells, using Cytochrome c antibody (#TA312385).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Western blot analysis of cytochrome c in Jurkat (left lane) and 3T3 (right lane) cell lysates. Rabbit Purified Hu, Ms, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Western blot analysis of cytochrome c expression in rat kidney tissue lysate. Rabbit Purified Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Intracellular staining of HeLa cells with Anti-Cytochrome C FITC. Appropriate isotype controls were used  (open histogram).  Total cells were used for analysis. Mouse 6H2 Purified Hu, Ms, Rt F
0.1 mg / €180.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Immunoblotting of cell lysates prepared from HeLa (lane 1) and Jurkat (lane 2) cell lines under reducing conditions using 5 ug/mL Anti-Cytochrome C Purified.  Bands were visualized using conjugated Anti-Mouse IgG HRP. Mouse 7H8 Purified Hu, Ms, Rt WB
0.1 mg / €180.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Western blot analysis of Cytochrome c expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Western blot analysis on COS7 cell lysate using Cytochrome c Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-cytochrome C (clone 7H8.2C12) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2a 7H8.2C12 Purified Eq, Hu, Ms, Rt WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Western Blot: Cytochrome C Antibody (7H8.2C12) [TA336369] - Analysis using cytochrome C antibody. Human HeLa lysate probed with cytochrome C antibody at 0.1 ug/ml. Mouse IgG2b 7H8.2C12 Purified Can, Dros, Eq, Hu, Mam, Ms C, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

Immunoprecipitation: Cytochrome C Antibody (6H2.B4) [TA337073] - Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2.B4 antibody/1 x107 cell equivalents. Mouse IgG1 6H2.B4 Purified Hu, Ms, Rt C, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYCS (RC209724, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYCS. Mouse IgG1 OTI2F2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cytochrome c (Holo) antibody

Cytochrome c Mouse IgG1 2CYZ-702 Purified Hu, Ms, Rt IP, WB
0.1 mg / €460.00
  Acris Antibodies GmbH

Cytochrome c antibody

Cytochrome c Mouse IgG1 2G8 Purified Hu, Ms, Rb, Rt E, F
0.1 mg / €350.00
  Acris Antibodies GmbH

Cytochrome c antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-cytochrome C (clone 7H8.2C12) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2a 7H8.2C12 Purified Eq, Hu, Ms, Rt WB
50 µl / €210.00
  Acris Antibodies GmbH

Cytochrome c Control Peptide

Cytochrome c Purified
50 µg / €190.00
  Acris Antibodies GmbH

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