Cytochrome c antibody

Principal name

Cytochrome c antibody

Alternative names for Cytochrome c antibody

CYCS, CYC

SwissProt ID

P00004 (Horse), P00011 (Canfa), P62894 (Bovin), P62897 (Mouse), P62898 (Rat), P99999 (Human)

Gene ID

54205 (CYCS), 13063 (Cycs), 100363502 (LOC100363502), 100847700 (LOC100847700), 25309

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit), Bov (Bovine), Gt (Goat), Mky (Monkey), Sh (Sheep), Eq (Equine), Chk (Chicken), Por (Porcine), Dros (Drosophila), Mam (Mammalia)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R), Functional assay (FN)

Background of Cytochrome c antibody

Human somatic cytochrome c is a 15 kDa, 104 amino acid (aa) polypeptide that participates in both oxidative phosphorylation and apoptosis . It exhibits 91% aa identity with mouse/rat cytochrome c . The molecule is initially synthesized in the cytoplasm as an extended, 12 kDa apoprotein. This molecule is subsequently transported across the outer mitochondrial membrane into the intermitochondrial space via a receptor-independent process. Here, it associates with an inner mitochondrial membrane enzyme called CCHL (cytochrome c heme lyase) which covalently attaches one heme molecule to the N-terminus, forming functional cytochrome c . This covalent attachment causes cytochrome c to undergo a conformational change to a globular molecule effectively trapping cytochrome c in the intermitochrondrial space.

Within the intermitochondrial space, cytochrome c is actively involved in the oxidative phosphorylation pathway. It transports electrons from the cytochrome c reductase complex to the cytochrome c oxidase complex . This transports excess electrons along the respiratory pathway and generates ATP for energy-dependent processes. Alternatively, and in response to apoptotic signals, cytochrome c can be released from mitochondria into the cytosol. Here, it activates an apoptotic program via one of many possible caspase-driven cascades . The events which trigger an apoptotic signal (such as growth factor withdrawal) are not well understood. The result of such a signal, however, has been suggested to be a translocation of cytosolic Bax and/or Bad to the outer mitochondrial membrane where they overcome a Bcl-2-mediated stabilization . Bax-mediated destabilization may take the form of channel creation or mitochondrial swelling, resulting in cytochrome c release . Whatever the mechanism, released cytochrome c participates in the formation of a cytosolic complex which is composed of 15 kDa cytochrome c, 130 kDa Apaf-1 (apoptotic protease activating factor-1), dATP, and 46 kDa Apaf-3/caspase-9 . Within this complex, Apaf-3/caspase-9 is activated, leading to the downstream activation of caspases-3,-7 and -9, followed by additional caspases that ultimately lead to cellular apoptosis .

General readings

Evans, M.J. and R.C. Scarpulla (1988) Proc. Natl. Acad. Sci. USA 85:9625.

Carlson, S.S. et al. (1977) Biochemistry 16:1437.

Virbasius, J.V. and R.C. Scarpulla (1988) J. Biol. Chem. 263:6791.

Cai, J. et al. (1998) Biochem. Biophys. Acta 1366:139.

Liu, X. et al. (1996) Cell 86:147.

Mayer, A. et al. (1995) J. Biol. Chem. 270:12390.

Stryer, L. (1981) in Biochemistry, W.H. Freeman & Co, p. 307.

Skulachev, V.P. (1998) FEBS Lett. 423:275.

Hatefi, Y. (1985) Annu. Rev. Immunol. 54:1015.

Tschopp, J. et al. (1998) Curr. Opin. Gen. Develop. 8:82.

Slee, E.A. et al. (1999) J. Cell Biol. 144:281.

Hakem, R. et al. (1998) Cell 94:339.

Gross, A. et al. (1998) EMBO J. 17:3878.

Garland, J.M. and C. Rudin (1998) Blood 92:1235.

Reed, J.C. et al. (1998) Biochem. Biophys. Acta 1366:127.

Jurgensmeier, J.M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4997.

Kluck, R.M. et al. (1997) Science 275:1132.

Reed, J.C. (1997) Cell 91:559.

Green, D.R. and J.C. Reed (1998) Science 281:1309.

Pan, G. et al. (1998) FEBS Lett. 426:151.

Kuida, K. et al. (1998) Cell 94:325.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cytochrome c antibody

  Mouse Monoclonal anti-Cytochrome c Antibody  
HeLa  cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 ug/ml of purified cytochrome c (clone 6H2.B4) in blocking buffer overnight at 4°C, and followed by DyLight™ 594 anti-mouse IgG (red) staining for 2 hours at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective. Mouse IgG1 6H2.B4 Purified Hu, Ms, Rt F, FN, ICC/IF, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Rabbit anti-CYCS Polyclonal Antibody  
Western blot analysis of extracts of mouse kidney cell line, using CYCS antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Rabbit polyclonal Cytochrome c antibody  
Western blot analysis of extracts from COS7 cells, using Cytochrome c antibody (#TA312385).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Rabbit polyclonal anti-Cytochrome c antibody  
Western blot analysis of cytochrome c in Jurkat (left lane) and 3T3 (right lane) cell lysates. Rabbit Purified Hu, Ms, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Rabbit polyclonal anti-Cytochrome c antibody  
Western blot analysis of cytochrome c expression in rat kidney tissue lysate. Rabbit Purified Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Mouse Anti-Cytochrome C Purified (100 ug)  
Intracellular staining of HeLa cells with Anti-Cytochrome C FITC. Appropriate isotype controls were used  (open histogram).  Total cells were used for analysis. Mouse 6H2 Purified Hu, Ms, Rt F
0.1 mg / €180.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Mouse Anti-Cytochrome C Purified (100 ug)  
Immunoblotting of cell lysates prepared from HeLa (lane 1) and Jurkat (lane 2) cell lines under reducing conditions using 5 ug/mL Anti-Cytochrome C Purified.  Bands were visualized using conjugated Anti-Mouse IgG HRP. Mouse 7H8 Purified Hu, Ms, Rt WB
0.1 mg / €180.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Rabbit Polyclonal Cytochrome c Antibody  
Western blot analysis of Cytochrome c expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Rabbit Polyclonal Cytochrome c Antibody  
Western blot analysis on COS7 cell lysate using Cytochrome c Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Mouse Monoclonal anti-Cytochrome c Antibody  
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-cytochrome C (clone 7H8.2C12) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2a 7H8.2C12 Purified Eq, Hu, Ms, Rt WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Mouse Monoclonal Cytochrome c Antibody (7H8.2C12)  
Western Blot: Cytochrome C Antibody (7H8.2C12) [TA336369] - Analysis using cytochrome C antibody. Human HeLa lysate probed with cytochrome C antibody at 0.1 ug/ml. Mouse IgG2b 7H8.2C12 Purified Can, Dros, Eq, Hu, Mam, Ms C, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Mouse Monoclonal Cytochrome c Antibody (6H2.B4)  
Immunoprecipitation: Cytochrome C Antibody (6H2.B4) [TA337073] - Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2.B4 antibody/1 x107 cell equivalents. Mouse IgG1 6H2.B4 Purified Hu, Ms, Rt C, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  Mouse Monoclonal anti-Cytochrome c Antibody  
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-cytochrome C (clone 7H8.2C12) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2a 7H8.2C12 Purified WB
50 µl / €248.00
  OriGene Technologies, Inc.

Cytochrome c antibody

  CYCS mouse monoclonal antibody, clone OTI2F2 (formerly 2F2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYCS (RC209724, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYCS. Mouse IgG1 OTI2F2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cytochrome c (Holo) antibody

Cytochrome c Mouse IgG1 2CYZ-702 Purified Hu, Ms, Rt IP, WB
0.1 mg / €460.00
  Acris Antibodies GmbH

Cytochrome c antibody

Cytochrome c Mouse IgG1 2G8 Purified Hu, Ms, Rb, Rt E, F
0.1 mg / €350.00
  Acris Antibodies GmbH

Cytochrome c Control Peptide

Cytochrome c Purified
50 µg / €190.00
  Acris Antibodies GmbH

Cytochrome c antibody

Western blot analysis of cytochrome c with mouse small intestine tissue lysate. Rabbit Purified Ms WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Cytochrome c antibody

Cytochrome c Sheep IgG Purified Can, Hu, Rb, Rt C, IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Cytochrome c antibody

Immunohistochemical analysis in paraffin-embedded human colon carcinoma tissue using Cytochrome c antibody (Cat.-No, AP06085PU-N). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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