Cytohesin 1 antibody

Principal name

Cytohesin 1 antibody

Alternative names for Cytohesin 1 antibody

CYTH1, D17S811E, PSCD1, SEC7 homolog B2-1, PH, SEC7 and coiled-coil domain-containing protein 1

SwissProt ID

P97694 (Rat), Q15438 (Human), Q9QX11 (Mouse)

Gene ID

9267 (CYTH1)

Available reactivities

Hu (Human), Ms (Mouse), Ze (Zebrafish), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Whole mount Immunohistochemistry (IHC-wm)

Background of Cytohesin 1 antibody

ARFs (ADP ribosylation factors) are GTP-binding proteins (20 kDa), which catalyze ADP-ribosylation of the alpha-subunit of the adenylyl cyclase-stimulatory G protein. ARFs are active when GTP, but not GDP or ATP, is bound. Hydrolysis of bound GTP to GDP with assistance of GTPase-activating protein results in inactive ARF-GDP. Conversion of ARF-GDP to ARFGTP is promoted by GEP (guanine-exchange protein). Inhibition of GEP activity by brefeldin A (BFA), a fungal metabolite that reversibly causes apparent disintegration of Golgi in cells, has been reported. ARFs have been shown to regulate various aspects of vesicular trafficking pathways in mammalian cells, including endocytosis, phagocytosis, secretion, and endoplasmic reticulum protein transport and budding of transport vesicles from Golgi in both anterograde and retrograde classes (class I: ARFs 1, 2 and 3, class II: ARFs 4 and 5, and class III: ARF 6), based on size, gene structure and sequence identity. The ARF-GEP family, referred to as 'cytohesins', include cytohesin 1, ARNO (ARF nucleotide binding site opener, also called cytohesin 2), and GRP1 (general receptor for phosphoinositides-1, also known as cytohesin 3 or ARNO3). The members of this family are characterized by an N-terminal coiled-coil domain of 40 amino acids, a Ptdlns (3,4,5)P3-binding C-terminal PH (pleckstrin homology) domain, and a central Sec7 domain. Sec7 is a conserved catalytic domain of approximately 200 amino acids, which stimulates the exchange of GDP to GTP on members of the ARF family of GTPases. The PH domain, by interacting with phospholipids, is believed to be responsible for association of cytoplasm with membranes. Cytohesin 1, the protein product of the B2-1 gene, is a 47 kDa protein. It is abundant in cells of the immune system and is expressed at high levels in natural killer cells and peripheral T lymphocytes, and also expressed at very low levels in purified monocytes and several cultured cell lines. Cytohesin 1 contains two structural motifs: a large central Sec7 domain (200 amino acids) and a carboxy-terminal PH domain (100 amino acids). Overexpression of a cytohesin 1 Sec7 domain fusion protein in Jurkat cells markedly increases cell binding to the extracellular domain of ICAM-1, and direct interaction of the Sec7 domain with the cytoplasmic domain of beta1 integrin was demonstrated in vitro. Cytohesin 1 acts as a GEP that accelerates GTP(yS) and GDP binding to purified native ARF3, and induces ARF binding to Golgi membranes via a mechanism insensitive to BFA. Via its PH domain, cytohesin 1 can bind the lipid product of PI3-Kinase activation, a binding that is visualized through the recruitment of cytohesin-1 to the plasma membrane. Also, granulocytic differentiation of HL-60 cells induced by cAMP results in a marked increase of the levels of endogenous cytohesin 1.

General readings

General / background references:Ogasawara M et al. Similarities in function and gene structure of cytohesin-4 and cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation factors. J Biol Chem 275:3221-30 (2000). PubMed PMID: 10652308
Venkateswarlu K et al. EGF-and NGF-stimulated translocation of cytohesin-1 to the plasma membrane of PC12 cells requires PI 3-kinase activation and a functional cytohesin-1 PH domain. J Cell Sci 112 ( Pt 12):1957-65 (1999). PubMed PMID: 10341214
Pacheco-Rodriguez G et al. Guanine nucleotide exchange on ADP-ribosylation factors catalyzed by cytohesin-1 and its Sec7 domain. J Biol Chem 273:26543-8 (1998). PubMed PMID: 9756891
Meacci E et al. Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor. Proc Natl Acad Sci U S A 94:1745-8 (1997). PubMed PMID: 9050849

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cytohesin 1 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1D6 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Cytohesin 1 antibody

Western Blot analysis of CYTH1 expression in transfected 293T cell line (H00009267-T01) by CYTH1 MaxPab polyclonal antibody.

Lane 1: PSCD1 transfected lysate(43.78 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu, Ms E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Cytohesin 1 antibody

Western Blot analysis of CYTH1 expression in transfected 293T cell line (H00009267-T02) by CYTH1 MaxPab polyclonal antibody.

Lane 1: PSCD1 transfected lysate(46.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Cytohesin 1 antibody

Immunoperoxidase of monoclonal antibody to PSCD1 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml] Mouse IgG1 1D6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cytohesin 1 antibody

Cytohesin 1 Mouse IgG1 CYT1-82 Purified Hu E, ICC/IF, WB
50 µl / €340.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cytohesin 1 (transcript variant 1)

Cytohesin 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Cytohesin 1 (1-398, His-tag)

Cytohesin 1 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Cytohesin 1 (1-398, His-tag)

Cytohesin 1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Cytohesin 1

Cytohesin 1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Cytohesin 1

Cytohesin 1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CYTH1 overexpression lysate

CYTH1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

CYTH1 overexpression lysate

CYTH1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

CYTH1 overexpression lysate

CYTH1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

PSCD1 293T Cell Transient Overexpression Lysate(Denatured)

PSCD1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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