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Cytohesin 2 antibody

Principal name

Cytohesin 2 antibody

Alternative names for Cytohesin 2 antibody

PSCD2, PSCD2L, ARNO, CYTH2, PH, SEC7 and coiled-coil domain-containing protein 2, ARF nucleotide-binding site opener, ARF exchange factor

SwissProt ID

P63034 (Mouse), P63035 (Rat), Q2KI41 (Bovin), Q99418 (Human)

Gene ID

9266 (CYTH2), 19158 (Cyth2), 116692

Ncbi ID

9606, NP_059431, NP_004219, NP_004219.3, NM_017457, NP_001106171.1, NM_011181, NM_001112701, NM_053911, NM_004228

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Bov (Bovine), Por (Porcine), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of Cytohesin 2 antibody

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins.
Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.
Like other small GTPases, Arf is found to be active when bound to GTP and inactive when bound to GDP. Arf’s activation is dependent upon guanine nucleotide exchange factors (GEFs) which increase the rate of exchange of bound GDP with GTP. All GEFs have a highly conserved Sec7 domain. GEF activity lies in the Sec7 domain and this activity has been shown to be inhibited by the fungal metabolite brefeldin-A (BFA). A small group of GEFs which are insensitive to brefeldin-A (BFA) include cytohesin-1 (B2-1), cytohesin-2 (ARNO), cytohesin-3 (ARNO3), and cytohesin-4. All cytohesins function in the cell periphery and contain a pleckstrin homology (PH) domain. The PH domain has been shown to interact with phosphatidylinositol 3,4,5-triphosphate and is believed to promote membrane targeting of the cytohesins. Recruitment of the cytohesins to the membranes can occur in response to tyrosine kinase receptor activation. This response appears to require the activation of phosphatidylinositol 3-kinase (PI 3-kinase).

General readings

Merkulova, M., et al. Biochim. Biophys. Acta 1797(8):1398-1409 (2010).
Torii, T., et al. J. Biol. Chem. 285(31):24270-24281 (2010).
White, D.T., et al. Mol. Biol. Cell 21(4):562-571 (2010).
Bouschet, T., et al. J. Cell. Sci. 120 (PT 15), 2489-2497 (2007).
Wu, C., et al. Proteomics 7(11):1775-1785 (2007).

Immunoperoxidase of monoclonal antibody to PSCD2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

H00009266-M02

Immunofluorescence of monoclonal antibody to PSCD2 on HeLa cell. [antibody concentration 10 ug/ml]

H00009266-M02

PSCD2 monoclonal antibody (M02), clone 6H5 Western Blot analysis of PSCD2 expression in PC-12 ( Cat # L012V1 ).

H00009266-M02

Western Blot analysis of CYTH2 expression in transfected 293T cell line by PSCD2 monoclonal antibody (M02), clone 6H5.Lane 1: CYTH2 transfected lysate(46.5 KDa).Lane 2: Non-transfected lysate.

H00009266-M02

Detection limit for recombinant GST tagged PSCD2 is approximately 0.03ng/ml as a capture antibody.

H00009266-M02

Western Blot analysis of CYTH2 expression in transfected 293T cell line (H00009266-T01) by CYTH2 MaxPab polyclonal antibody.Lane 1: PSCD2 transfected lysate(44 KDa).Lane 2: Non-transfected lysate.

H00009266-B01

CYTH2 polyclonal antibody ( Cat # PAB7405 ) ( 0.5 µg/ml ) staining of human brain ( cerebellum ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7405

Western Blot analysis of CYTH2 expression in transfected 293T cell line (H00009266-T02) by CYTH2 MaxPab polyclonal antibody.Lane 1: PSCD2 transfected lysate(46.50 KDa).Lane 2: Non-transfected lysate.

H00009266-D01P

CYTH2 monoclonal antibody (M01), clone 5E11. Western Blot analysis of CYTH2 expression in A-431(Cat # L015V1 ).

H00009266-M01

Immunoprecipitation of CYTH2 transfected lysate using anti-CYTH2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CYTH2 MaxPab mouse polyclonal antibody (B01) (H00009266-B01).

H00009266-D01

Western blot analysis of PSCD2 Antibody (N-term) Cat.-No AP53479PU-N in mouse kidney tissue lysates (35ug/lane). This demonstrates the PSCD2 antibody detected the PSCD2 protein (arrow).

AP53479PU-N

Immunohistochemistry: Cytohesin 2 Antibody - Most of the normal tissues displayed weak to moderate cytoplasmic positivity. Distinct cytoplasmic staining was seen in a fraction of cells in red pulp of spleen and distinct membranous positivity was seen in trophoblasts. Squamous epithelia, urothelia, kidney, myocytes and cells of the CNS were mainly negative. Malignant tissues were negative or showed weak cytoplasmic immunoreactivity. A single case of lymphoma exhibited strong staining. Melanomas and urothelial cancers were predominantly weakly to moderately stained. Image and statement courtesy of the Human Protein Atlas (HPA).

TA590845

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CYTH2 Rabbit Polyclonal antibody. (TA590845)

TA590845

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CYTH2 Rabbit Polyclonal antibody. (TA590845)

TA590845

Western Blot: Cytohesin 2 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 47, 46 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

TA590845

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYTH2 (RC207179, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYTH2.

TA590845

ELISA: Cytohesin 2 Antibody

TA590845

TP317980

TA303303 (0.5µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303303

Host: Rabbit; Target Name: CYTH2; Sample Tissue: MDA-MB-435S Whole Cell lysates; Antibody Dilution: 1.0 ug/ml

TA335544

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA590845	Cytohesin 2 antibody
	Rabbit Polyclonal Cytohesin 2 Antibody
$Immunohistochemistry: Cytohesin 2 Antibody - Most of the normal tissues displayed weak to moderate cytoplasmic positivity. Distinct cytoplasmic staining was seen in a fraction of cells in red pulp of spleen and distinct membranous positivity was seen in trophoblasts. Squamous epithelia, urothelia, kidney, myocytes and cells of the CNS were mainly negative. Malignant tissues were negative or showed weak cytoplasmic immunoreactivity. A single case of lymphoma exhibited strong staining. Melanomas and urothelial cancers were predominantly weakly to moderately stained. Image and statement courtesy of the Human Protein Atlas (HPA).$	Rabbit	IgG	Purified	Hu	E, P, WB	0.1 mg / €325.00
						OriGene Technologies, Inc.
+5 additional images	Highly validated antibody!
TA303303	Cytohesin 2 (N-term) antibody
	Goat Polyclonal Antibody against PSCD2
	Goat		Aff - Purified	Can, Hu, Ms, Rt	WB	0.1 mg / €325.00
						OriGene Technologies, Inc.
TA335544	Cytohesin 2 antibody
	Rabbit Polyclonal Anti-CYTH2 Antibody
	Rabbit	IgG	Purified	Bov, Hu, Ms, Por, Rt, Sh, Ze	WB	50 µg / €325.00
						OriGene Technologies, Inc.
AP53479PU-N	Cytohesin 2 (N-term) antibody
	Rabbit	Ig	Aff - Purified	Hu, Ms	WB	0.4 ml / €370.00
						Acris Antibodies GmbH

CRISPR

Catalog No.	Species	Donor Vector
KN207179	CYTH2 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,200.00
			OriGene Technologies, Inc.
KN207179BN	CYTH2 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN207179LP	CYTH2 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN207179RB	CYTH2 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN304204	Cyth2 - mouse gene knockout kit via CRISPR
	Mouse	GFP-Puro	1 kit / €1,200.00
			OriGene Technologies, Inc.
KN304204BN	Cyth2 - mouse gene knockout kit via CRISPR
	Mouse	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN304204LP	Cyth2 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN304204RB	Cyth2 - mouse gene knockout kit via CRISPR
	Mouse	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP317980	Cytohesin 2 (transcript variant 1)
	Recombinant protein of human cytohesin 2 (CYTH2), transcript variant 1
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00
					OriGene Technologies, Inc.

AR50576PU-N	Cytohesin 2 (1-399, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.5 mg / €730.00
					Acris Antibodies GmbH
AR50576PU-S	Cytohesin 2 (1-399, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.1 mg / €300.00
					Acris Antibodies GmbH

Lysates

Catalog No.
LY413761	CYTH2 overexpression lysate
		0.1 mg / €280.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC217980L2V	Lenti ORF particles, CYTH2 (mGFP-tagged) - Human cytohesin 2 (CYTH2), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €1,050.00
		OriGene Technologies, Inc.

RC207179L2V	Lenti ORF particles, CYTH2 (mGFP-tagged)-Human cytohesin 2 (CYTH2), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €1,050.00
		OriGene Technologies, Inc.

RC217980L1V	Lenti ORF particles, CYTH2 (Myc-DDK tagged) - Human cytohesin 2 (CYTH2), transcript variant 1 , 200 uL, >10^7 TU/mL
		200 µl / €940.00
		OriGene Technologies, Inc.

RC207179L1V	Lenti ORF particles, CYTH2 (Myc-DDK-tagged)-Human cytohesin 2 (CYTH2), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €940.00
		OriGene Technologies, Inc.

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