Cytohesin 2 antibody

Principal name

Cytohesin 2 antibody

Alternative names for Cytohesin 2 antibody

PSCD2, PSCD2L, ARNO, CYTH2, PH, SEC7 and coiled-coil domain-containing protein 2, ARF nucleotide-binding site opener, ARF exchange factor

SwissProt ID

P63034 (Mouse), P63035 (Rat), Q2KI41 (Bovin), Q99418 (Human)

Gene ID

9266 (CYTH2), 19158 (Cyth2), 116692

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Bov (Bovine), Por (Porcine), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of Cytohesin 2 antibody

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins.
Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.
Like other small GTPases, Arf is found to be active when bound to GTP and inactive when bound to GDP. Arf’s activation is dependent upon guanine nucleotide exchange factors (GEFs) which increase the rate of exchange of bound GDP with GTP. All GEFs have a highly conserved Sec7 domain. GEF activity lies in the Sec7 domain and this activity has been shown to be inhibited by the fungal metabolite brefeldin-A (BFA). A small group of GEFs which are insensitive to brefeldin-A (BFA) include cytohesin-1 (B2-1), cytohesin-2 (ARNO), cytohesin-3 (ARNO3), and cytohesin-4. All cytohesins function in the cell periphery and contain a pleckstrin homology (PH) domain. The PH domain has been shown to interact with phosphatidylinositol 3,4,5-triphosphate and is believed to promote membrane targeting of the cytohesins. Recruitment of the cytohesins to the membranes can occur in response to tyrosine kinase receptor activation. This response appears to require the activation of phosphatidylinositol 3-kinase (PI 3-kinase).

General readings

Merkulova, M., et al. Biochim. Biophys. Acta 1797(8):1398-1409 (2010).
Torii, T., et al. J. Biol. Chem. 285(31):24270-24281 (2010).
White, D.T., et al. Mol. Biol. Cell 21(4):562-571 (2010).
Bouschet, T., et al. J. Cell. Sci. 120 (PT 15), 2489-2497 (2007).
Wu, C., et al. Proteomics 7(11):1775-1785 (2007).

  1. Quick-Filters:
  2. Highly Validated (2)

Items 1 to 20 of 23 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cytohesin 2 antibody

Immunohistochemistry: Cytohesin 2 Antibody - Most of the normal tissues displayed weak to moderate cytoplasmic positivity. Distinct cytoplasmic staining was seen in a fraction of cells in red pulp of spleen and distinct membranous positivity was seen in trophoblasts. Squamous epithelia, urothelia, kidney, myocytes and cells of the CNS were mainly negative. Malignant tissues were negative or showed weak cytoplasmic immunoreactivity. A single case of lymphoma exhibited strong staining. Melanomas and urothelial cancers were predominantly weakly to moderately stained.  Image and statement courtesy of the Human Protein Atlas (HPA). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytohesin 2 antibody

Immunoperoxidase of monoclonal antibody to PSCD2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG2a 6H5 Purified Hu, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cytohesin 2 (N-term) antibody

TA303303 (0.5µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytohesin 2 antibody

Host: Rabbit; Target Name: CYTH2; Sample Tissue: MDA-MB-435S Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Hu, Ms, Por, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cytohesin 2 (N-term) antibody

Western blot analysis of PSCD2 Antibody (N-term) Cat.-No AP53479PU-N in mouse kidney tissue lysates (35ug/lane). This demonstrates the PSCD2 antibody detected the PSCD2 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Cytohesin 2 antibody

Western Blot analysis of CYTH2 expression in transfected 293T cell line (H00009266-T01) by CYTH2 MaxPab polyclonal antibody.

Lane 1: PSCD2 transfected lysate(44 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Cytohesin 2 antibody

Immunoprecipitation of CYTH2 transfected lysate using anti-CYTH2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CYTH2 MaxPab mouse polyclonal antibody (B01) (H00009266-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Cytohesin 2 antibody

Western Blot analysis of CYTH2 expression in transfected 293T cell line (H00009266-T02) by CYTH2 MaxPab polyclonal antibody.

Lane 1: PSCD2 transfected lysate(46.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Cytohesin 2 antibody

CYTH2 monoclonal antibody (M01), clone 5E11. Western Blot analysis of CYTH2 expression in A-431(Cat # L015V1 ). Mouse IgG2b 5E11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cytohesin 2 antibody

CYTH2 polyclonal antibody ( Cat # PAB7405 ) ( 0.5 µg/ml ) staining of human brain ( cerebellum ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cytohesin 2 (transcript variant 1)

Cytohesin 2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Cytohesin 2 (1-399, His-tag)

Cytohesin 2 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Cytohesin 2 (1-399, His-tag)

Cytohesin 2 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Cytohesin 2

Cytohesin 2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Cytohesin 2

Cytohesin 2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Cytohesin 2

Cytohesin 2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Cytohesin 2

Cytohesin 2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

CYTH2 (Human) IP-WB Antibody Pair

CYTH2 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CYTH2 overexpression lysate

CYTH2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

PSCD2 293T Cell Transient Overexpression Lysate(Denatured)

PSCD2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

Items 1 to 20 of 23 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn