Cytohesin 2 antibody

Principal name

Cytohesin 2 antibody

Alternative names for Cytohesin 2 antibody

PSCD2, PSCD2L, ARNO, CYTH2, PH, SEC7 and coiled-coil domain-containing protein 2, ARF nucleotide-binding site opener, ARF exchange factor

SwissProt ID

P63034 (Mouse), P63035 (Rat), Q2KI41 (Bovin), Q99418 (Human)

Gene ID

9266 (CYTH2)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Bov (Bovine), Por (Porcine), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of Cytohesin 2 antibody

ARFs (ADP ribosylation factors) are GTP-binding proteins (20 kDa), which catalyze ADP-ribosylation of the alpha-subunit of the adenylyl cyclase-stimulatory G protein. ARFs have been shown to regulate various aspects of vesicular trafficking pathways in mammalian cells, including endocytosis, phagocytosis, secretion, and endoplasmic reticulum protein transport and budding of transport vesicles from Golgi in both anterograde and retrograde directions. Mammalian ARFs are grouped into three classes (class I: ARFs 1, 2, and 3, class II: ARFs 4 and 5, and class III: ARF 6), based on size, gene structure, and sequence identity. ARFs are active when GTP, but not GDP or ATP, is bound. Hydrolysis of bound GTP to GDP with assistance of GTPase-activating protein, results in inactive ARF-GDP. Conversion of ARF-GDP to ARFGTP is promoted by GEP (guanine-exchange protein). The ARF-GEP family of guanine nucleotide-exchange proteins (also referred to as cytohesins) includes cytohesin 1, ARNO (ARF nucleotide binding site opener, also called cytohesin 2 or Sec7p), and GRP1 (general receptor for phosphoinositides-1, also known as cytohesin 3 or ARNO3). ARF-GEP family members are characterized by an N-terminal coiled-coil domain of 40 amino acids, a PtdIns(3,4,5)P3-binding C-terminal PH (pleckstrin homology) domain, and a central Sec7 domain. Sec7 is a conserved catalytic domain of approximately 200 amino acids, which stimulates the exchange of GDP to GTP on members of the ARF family of GTPases. The PH domain, by interacting with phospholipids, is believed to be responsible for association of cytoplasm with membranes. ARNO (47 kDa) appears as a dimer of approximately 90 kDa in gel filteration, but has an apparent molecular weight of approximately 40 kDa in SDS-PAGE. It is 83% homologous to cytohesin 1. However, while cytohesin 1 expression appears to be limited to hematopoietic cells, ARNO is more ubiquitously expressed. The catalytic activity of ARNO has been localized to the region of the Sec7 domain, and it appears to be positively regulated by interaction of the PH domain with inositol phospholipids. ARNO is localized to the plasma membrane in most mammlian cells, where it functions as an exchange factor for the plasma membrane-located ARF6 rather than ARF1. In HeLa cells, ARNO is mostly cytosolic, and its recruitment from cytosol to endosomes in the epithelial cells of the kidney, is pH-dependent.

General readings

General / background references:Maranda B et al. Intra-endosomal pH-sensitive recruitment of the Arf-nucleotide exchange factor ARNO and Arf6 from cytoplasm to proximal tubule endosomes. J Biol Chem 276:18540-50 (2001). PubMed PMID: 11278939
Monier S et al. Overexpression of the ARF1 exchange factor ARNO inhibits the early secretory pathway and causes the disassembly of the Golgi complex. J Cell Sci 111 ( Pt 22):3427-36 (1998). PubMed PMID: 9788883
Venkateswarlu K et al. Insulin-dependent translocation of ARNO to the plasma membrane of adipocytes requires phosphatidylinositol 3-kinase. Curr Biol 8:463-6 (1998). PubMed PMID: 9550703
Frank S et al. ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6. J Biol Chem 273:23-7 (1998). PubMed PMID: 9417041

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cytohesin 2 antibody

Immunohistochemistry: Cytohesin 2 Antibody - Most of the normal tissues displayed weak to moderate cytoplasmic positivity. Distinct cytoplasmic staining was seen in a fraction of cells in red pulp of spleen and distinct membranous positivity was seen in trophoblasts. Squamous epithelia, urothelia, kidney, myocytes and cells of the CNS were mainly negative. Malignant tissues were negative or showed weak cytoplasmic immunoreactivity. A single case of lymphoma exhibited strong staining. Melanomas and urothelial cancers were predominantly weakly to moderately stained.  Image and statement courtesy of the Human Protein Atlas (HPA). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytohesin 2 antibody

Immunoperoxidase of monoclonal antibody to PSCD2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG2a 6H5 Purified Hu, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cytohesin 2 (N-term) antibody

TA303303 (0.5µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cytohesin 2 antibody

Host: Rabbit; Target Name: CYTH2; Sample Tissue: MDA-MB-435S Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Hu, Ms, Por, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Cytohesin 2 (N-term) antibody

Western blot analysis of PSCD2 Antibody (N-term) Cat.-No AP53479PU-N in mouse kidney tissue lysates (35ug/lane). This demonstrates the PSCD2 antibody detected the PSCD2 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Cytohesin 2 antibody

Western Blot analysis of CYTH2 expression in transfected 293T cell line (H00009266-T01) by CYTH2 MaxPab polyclonal antibody.

Lane 1: PSCD2 transfected lysate(44 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Cytohesin 2 antibody

Immunoprecipitation of CYTH2 transfected lysate using anti-CYTH2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CYTH2 MaxPab mouse polyclonal antibody (B01) (H00009266-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Cytohesin 2 antibody

Western Blot analysis of CYTH2 expression in transfected 293T cell line (H00009266-T02) by CYTH2 MaxPab polyclonal antibody.

Lane 1: PSCD2 transfected lysate(46.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Cytohesin 2 antibody

CYTH2 monoclonal antibody (M01), clone 5E11. Western Blot analysis of CYTH2 expression in A-431(Cat # L015V1 ). Mouse IgG2b 5E11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Cytohesin 2 antibody

CYTH2 polyclonal antibody ( Cat # PAB7405 ) ( 0.5 µg/ml ) staining of human brain ( cerebellum ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Cytohesin 2 antibody

Immunohistochemistry: Cytohesin 2 Antibody [20590002] - Most of the normal tissues displayed weak to moderate cytoplasmic positivity. Distinct cytoplasmic staining was seen in a fraction of cells in red pulp of spleen and distinct membranous positivity was seen in trophoblasts. Squamous epithelia, urothelia, kidney, myocytes and cells of the CNS were mainly negative. Malignant tissues were negative or showed weak cytoplasmic immunoreactivity. A single case of lymphoma exhibited strong staining. Melanomas and urothelial cancers were predominantly weakly to moderately stained.  Image and statement courtesy of the Human Protein Atlas (HPA). Rabbit Hu E, P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Cytohesin 2 antibody

NB100-77340 (0.5µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Cytohesin 2 antibody

Rat brain extract was separated on SDS-PAGE and probed with Monoclonal PA to ARNO (Cytohesin-2) CL: CYT2-21. The antibody was developed with Biotin APA Mouse Fab ad/HuBov, Hor as a second Ab, Alkaline Phosphatase conjugate as a tertiary reagent and a NBT/BCIP substrate.  Antibody concentration 5 ìg/mL. Mouse IgG1 CYT2-21 Purified Hu, Rt E, ICC/IF, WB
50 µl / €340.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Cytohesin 2 (transcript variant 1)

Cytohesin 2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Cytohesin 2 (1-399, His-tag)

Cytohesin 2 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Cytohesin 2 (1-399, His-tag)

Cytohesin 2 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Cytohesin 2

Cytohesin 2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Cytohesin 2

Cytohesin 2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Cytohesin 2

Cytohesin 2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Cytohesin 2

Cytohesin 2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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